Inactivation of SARS-CoV-2 on surfaces and in solution with Virusend (TX-10), a novel disinfectant Open Access

Abstract

Until an effective vaccine against SARS-CoV-2 is available on a widespread scale, the control of the COVID-19 pandemic is reliant upon effective pandemic control measures. The ability of SARS-CoV-2 to remain viable on surfaces and in aerosols, means indirect contact transmission can occur and there is an opportunity to reduce transmission using effective disinfectants in public and communal spaces. Virusend (TX-10), a novel disinfectant, has been developed as a highly effective disinfectant against a range of microbial agents. Here we investigate the ability of Virusend to inactivate SARS-CoV-2. Using surface and solution inactivation assays, we show that Virusend is able to reduce SARS-CoV-2 viral titre by 4 log p.f.u. ml within 1 min of contact. Ensuring disinfectants are highly effective against SARS-CoV-2 is important in eliminating environmental sources of the virus to control the COVID-19 pandemic.

Funding
This study was supported by the:
  • Ministry of Defence
    • Principle Award Recipient: EdwardI. Patterson
  • Ministry of Defence
    • Principle Award Recipient: GrantL. Hughes
  • Liverpool School of Tropical Medicine (Award Director's Catalyst Fund)
    • Principle Award Recipient: EdwardI. Patterson
  • National Institute for Health Research (Award NIHR2000907)
    • Principle Award Recipient: GrantL. Hughes
  • UKRI (Award 20197)
    • Principle Award Recipient: GrantL. Hughes
  • National Institutes of Health (Award R21AI138074)
    • Principle Award Recipient: GrantL. Hughes
  • Royal Society Wolfson Fellowship (Award RSWF\R1\180013)
    • Principle Award Recipient: GrantL. Hughes
  • Biotechnology and Biological Sciences Research Council (Award V011278/1)
    • Principle Award Recipient: GrantL. Hughes
  • Biotechnology and Biological Sciences Research Council (Award BB/T001240/1)
    • Principle Award Recipient: GrantL. Hughes
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2021-04-26
2024-03-28
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References

  1. Wu F, Zhao S, Yu B, Chen Y-M, Wang W et al. A new coronavirus associated with human respiratory disease in China. Nature 2020; 579:265–269 [View Article][PubMed]
    [Google Scholar]
  2. Sharpe HR, Gilbride C, Allen E, Belij-Rammerstorfer S, Bissett C et al. The early landscape of coronavirus disease 2019 vaccine development in the UK and rest of the world. Immunology 2020; 160:223–232 [View Article][PubMed]
    [Google Scholar]
  3. Yamey G, Schäferhoff M, Hatchett R, Pate M, Zhao F et al. Ensuring global access to COVID-19 vaccines. Lancet 2020; 395:1405–1406 [View Article][PubMed]
    [Google Scholar]
  4. Thanh Le T, Andreadakis Z, Kumar A, Gómez Román R, Tollefsen S et al. The COVID-19 vaccine development landscape. Nat Rev Drug Discov 2020; 19:305–306 [View Article][PubMed]
    [Google Scholar]
  5. Cheng VC-C, Wong S-C, Chuang VW-M, So SY-C, Chen JH-K et al. The role of community-wide wearing of face mask for control of coronavirus disease 2019 (COVID-19) epidemic due to SARS-CoV-2. J Infect 2020; 81:107–114 [View Article][PubMed]
    [Google Scholar]
  6. Xiao S, Li Y, Wong T-W, Hui DSC. Role of fomites in SARS transmission during the largest hospital outbreak in Hong Kong. PLoS One 2017; 12:e0181558 [View Article][PubMed]
    [Google Scholar]
  7. van Doremalen N, Bushmaker T, Morris DH, Holbrook MG, Gamble A et al. Aerosol and surface stability of SARS-CoV-2 as compared with SARS-CoV-1. N Engl J Med 2020; 382:1564–1567 [View Article][PubMed]
    [Google Scholar]
  8. Patterson EI, Prince T, Anderson ER, Casas-Sanchez A, Smith SL et al. Methods of inactivation of SARS-CoV-2 for downstream biological assays. J Infect Dis 2020; 222:1462–1467 [View Article][PubMed]
    [Google Scholar]
  9. Patterson EI, Warmbrod KL, Bouyer DH, Forrester NL. Evaluation of the inactivation of Venezuelan equine encephalitis virus by several common methods. J Virol Methods 2018; 254:31–34 [View Article][PubMed]
    [Google Scholar]
  10. Chan K-H, Sridhar S, Zhang RR, Chu H, Fung AY-F et al. Factors affecting stability and infectivity of SARS-CoV-2. J Hosp Infect 2020; 106:226–231 [View Article][PubMed]
    [Google Scholar]
  11. Hellewell J, Abbott S, Gimma A, Bosse NI, Jarvis CI et al. Feasibility of controlling COVID-19 outbreaks by isolation of cases and contacts. Lancet Glob Health 2020; 8:e488-e96e488–e496 [View Article][PubMed]
    [Google Scholar]
  12. Ogbunugafor CB, Miller-Dickson MD, Meszaros VA, Gomez LM, Murillo AL et al. Variation in microparasite free-living survival and indirect transmission can modulate the intensity of emerging outbreaks. Sci Rep 2020; 10:1–14 [View Article]
    [Google Scholar]
  13. Xiling G, Yin C, Ling W, Xiaosong W, Jingjing F et al. In vitro inactivation of SARS-CoV-2 by commonly used disinfection products and methods. Sci Rep 2021; 11:2418 PMID [View Article][PubMed]
    [Google Scholar]
  14. WHO Infection Prevention and Control of Epidemic- and Pandemic-prone Acute Respiratory Infections in Health Care Geneva: WHO Guidelines Approved by the Guidelines Review Committee; 2014
    [Google Scholar]
  15. Song H, Li J, Shi S, Yan L, Zhuang H et al. Thermal stability and inactivation of hepatitis C virus grown in cell culture. Virol J 2010; 7:40 [View Article][PubMed]
    [Google Scholar]
  16. Razzini K, Castrica M, Menchetti L, Maggi L, Negroni L et al. SARS-CoV-2 RNA detection in the air and on surfaces in the COVID-19 ward of a hospital in Milan, Italy. Sci Total Environ 2020; 742:140540 [View Article][PubMed]
    [Google Scholar]
  17. Coley SE, Lavi E, Sawicki SG, Fu L, Schelle B et al. Recombinant mouse hepatitis virus strain A59 from cloned, full-length cDNA replicates to high titers in vitro and is fully pathogenic in vivo. J Virol 2005; 79:3097–3106 [View Article][PubMed]
    [Google Scholar]
  18. Richards GP. Critical review of norovirus surrogates in food safety research: rationale for considering volunteer studies. Food Environ Virol 2012; 4:6–13 [View Article][PubMed]
    [Google Scholar]
  19. The Academy of Medical Sciences Preparing for a challenging winter 2020/21; 2020 https://acmedsci.ac.uk/file-download/51353957
  20. Dear K, Grayson L, Nixon R. Potential methanol toxicity and the importance of using a standardised alcohol-based hand rub formulation in the era of COVID-19. Antimicrob Resist Infect Control 2020; 9:129 [View Article][PubMed]
    [Google Scholar]
  21. Klemeš JJ, Fan YV, Jiang P. The energy and environmental footprints of COVID-19 fighting measures - PPE, disinfection, supply chains. Energy 2020; 211:118701 [View Article][PubMed]
    [Google Scholar]
  22. Dicken RD, Gallagher T, Perks S. Overcoming the regulatory hurdles for the production of hand sanitizer for public health protection: the UK and US academic perspective. ACS Chem. Health Saf. 2020; 27:209–213 [View Article]
    [Google Scholar]
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