@article{mbs:/content/journal/acmi/10.1099/acmi.0.000179, author = "Decano, Arun Gonzales and Tran, Nghia and Al-Foori, Hawriya and Al-Awadi, Buthaina and Campbell, Leigh and Ellison, Kevin and Mirabueno, Louisse Paolo and Nelson, Maddy and Power, Shane and Smith, Genevieve and Smyth, Cian and Vance, Zoe and Woods, Caitriona and Rahm, Alexander and Downing, Tim", title = "Plasmids shape the diverse accessory resistomes of Escherichia coli ST131", journal= "Access Microbiology", year = "2021", volume = "3", number = "1", pages = "", doi = "https://doi.org/10.1099/acmi.0.000179", url = "https://www.microbiologyresearch.org/content/journal/acmi/10.1099/acmi.0.000179", publisher = "Microbiology Society", issn = "2516-8290", type = "Journal Article", keywords = "fimbrial", keywords = "ST131", keywords = "Genome", keywords = "Escherichia coli", keywords = "plasmid", keywords = "evolution", keywords = "infection", eid = "000179", abstract = "The human gut microbiome includes beneficial, commensal and pathogenic bacteria that possess antimicrobial resistance (AMR) genes and exchange these predominantly through conjugative plasmids. Escherichia coli is a significant component of the gastrointestinal microbiome and is typically non-pathogenic in this niche. In contrast, extra-intestinal pathogenic E. coli (ExPEC) including ST131 may occupy other environments like the urinary tract or bloodstream where they express genes enabling AMR and host cell adhesion like type 1 fimbriae. The extent to which commensal E. coli and uropathogenic ExPEC ST131 share AMR genes remains understudied at a genomic level, and we examined this here using a preterm infant resistome. We found that individual ST131 had small differences in AMR gene content relative to a larger shared resistome. Comparisons with a range of plasmids common in ST131 showed that AMR gene composition was driven by conjugation, recombination and mobile genetic elements. Plasmid pEK499 had extended regions in most ST131 Clade C isolates, and it had evidence of a co-evolutionary signal based on protein-level interactions with chromosomal gene products, as did pEK204 that had a type IV fimbrial pil operon. ST131 possessed extensive diversity of selective type 1, type IV, P and F17-like fimbriae genes that was highest in subclade C2. The structure and composition of AMR genes, plasmids and fimbriae vary widely in ST131 Clade C and this may mediate pathogenicity and infection outcomes.", }