1887

Abstract

DNA adenine methyltransferase () has been well documented for its role in regulation of replication, mismatch repair and transposition. Recent studies have also suggested a role for in protection against antibiotic stress, although this is not yet fully defined. We therefore evaluated the role of in the development of antibiotic resistance and triclosan-associated cross-resistance.

A significant impact on growth rate was seen in the knockout compared to the parental strain. Known triclosan resistance-associated mutations in were seen regardless of status, with an additional mutation in seen in the knockout. The expression of multiple antibiotic resistance-associated genes was significantly different between the parent and knockout post-resistance induction. Reversion rate assays showed that resistance mechanisms were stable.

knockout had a significant effect on growth, but its role in the development of antibiotic resistance is likely confined to those antibiotics using -containing efflux pumps.

Funding
This study was supported by the:
  • Leeds Beckett University
    • Principle Award Recipient: Not Applicable
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2020-11-18
2024-03-29
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