Sputum and endotracheal aspirates (ETs) are not among the vendor-approved specimens for the Cepheid Xpert SARS-CoV-2 assay. However, they are the common lower respiratory tract specimens submitted for laboratory diagnosis. Testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in lower respiratory tract samples is required for the discharge of patients from coronavirus disease (COVID) units at some institutions. We developed a protocol used for testing unliquified viscous sputum or tracheal aspirate with the Cepheid Xpert SARS-CoV-2 assay.


Article metrics loading...

Loading full text...

Full text loading...



  1. Wölfel R, Corman VM, Guggemos W, Seilmaier M, Zange S et al. Virological assessment of hospitalized patients with COVID-2019. Nature 2020; 581:465–469 [View Article][PubMed]
    [Google Scholar]
  2. Lan L, Xu D, Ye G, Xia C, Wang S et al. Positive RT-PCR test results in patients recovered from COVID-19. JAMA 2020; 323:1502 [View Article][PubMed]
    [Google Scholar]
  3. Cepheid Cepheid Xpert Xpress SARS-CoV-2 Instructions for Use. April 2020(Rev C). 3260
  4. FDA Statistical guidance on reporting results from studies evaluating diagnostic tests; 2007

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error