1887

Abstract

Clinical pathogens, especially Gram-negative bacteria developing resistance to third-generation cephalosporins, are making clinical outcomes more complicated and serious. This study was undertaken to evaluate the distribution of CTX-M-type extended-spectrum β-lactamases (ESBLs) in Tamil Nadu, India. For this study, clinical samples were collected from five different hospitals located in Tamil Nadu and the ESBL-producing Gram-negative isolates were characterized. MIC was performed using cefotaxime and ceftazidime. The -producing genes were screened using multiplex PCR for the genes, CTX-M group-1, -2, -8, -9, -26. The conjugation studies were performed using AB1157 as a recipient for the isolates harbouring plasmid-borne resistance following broth-mating experiment. In total, 1500 samples were collected and 599 Gram-negative bacteria were isolated that included (=233), (=182), (=79), spp. (=30), (=28), spp. (=21), (=12), . (=6), spp. (=4), (=3) and spp. (=1). MIC results showed that 358 isolates were resistant to cefotaxime and ceftazidime. Further, ESBL gene-amplification results showed that 19 isolates had CTX-M group-1 gene including (=16) (=2) and (=1) whereas one isolate had CTX-M group-9, which was plasmid-borne. Through conjugation studies, 12/20 isolates were found to be involved in the transformation of its plasmid-borne resistance gene. Our study highlighted the importance of horizontal gene transfer in the dissemination of plasmid-borne resistance genes among the clinical isolates.

Loading

Article metrics loading...

/content/journal/acmi/10.1099/acmi.0.000142
2020-06-11
2020-12-01
Loading full text...

Full text loading...

/deliver/fulltext/acmi/10.1099/acmi.0.000142/acmi000142.html?itemId=/content/journal/acmi/10.1099/acmi.0.000142&mimeType=html&fmt=ahah

References

  1. Paterson DL, Bonomo RA. Extended-Spectrum beta-lactamases: a clinical update. Clin Microbiol Rev 2005; 18: 657 686 [CrossRef] [PubMed]
    [Google Scholar]
  2. Rawat D, Nair D. Extended-Spectrum ß-lactamases in gram negative bacteria. J Glob Infect Dis 2010; 2: 263 [CrossRef]
    [Google Scholar]
  3. Cantón R, Coque TM. The CTX-M beta-lactamase pandemic. Curr Opin Microbiol 2006; 9: 466 475 [CrossRef] [PubMed]
    [Google Scholar]
  4. Paterson DL. Resistance in gram-negative bacteria: Enterobacteriaceae. Am J Med 2006; 119: S20 S28 [CrossRef] [PubMed]
    [Google Scholar]
  5. Livermore DM, Canton R, Gniadkowski M, Nordmann P, Rossolini GM et al. Ctx-M: changing the face of ESBLs in Europe. J Antimicrob Chemother 2007; 59: 165 174 [CrossRef] [PubMed]
    [Google Scholar]
  6. Jain A, Roy I, Gupta MK, Kumar M, Agarwal SK. Prevalence of extended-spectrum beta-lactamase-producing gram-negative bacteria in septicaemic neonates in a tertiary care hospital. J Med Microbiol 2003; 52: 421 425 [CrossRef] [PubMed]
    [Google Scholar]
  7. Upadhyay S, Hussain A, Mishra S, Maurya AP, Bhattacharjee A et al. Genetic environment of plasmid mediated CTX-M-15 extended spectrum beta-lactamases from clinical and food borne bacteria in north-eastern India. PLoS One 2015; 10: e0138056 [CrossRef] [PubMed]
    [Google Scholar]
  8. Salvatore DJ, Resman-Targoff BH. Treatment Options for Urinary Tract Infections Caused by Extended-Spectrum Β-Lactamase-Producing Escherichia coli and Klebsiella pneumonia . J Hosp Med 2015; 7:
    [Google Scholar]
  9. Livermore DM. Current epidemiology and growing resistance of gram-negative pathogens. Korean J Intern Med 2012; 27: 128 142 [CrossRef] [PubMed]
    [Google Scholar]
  10. Pitout JDD, Laupland KB. Extended-Spectrum beta-lactamase-producing Enterobacteriaceae: an emerging public-health concern. Lancet Infect Dis 2008; 8: 159 166 [CrossRef] [PubMed]
    [Google Scholar]
  11. Karim A, Poirel L, Nagarajan S, Nordmann P. Plasmid-Mediated extended-spectrum beta-lactamase (CTX-M-3 like) from India and gene association with insertion sequence ISEcp1. FEMS Microbiol Lett 2001; 201: 237 241 [CrossRef] [PubMed]
    [Google Scholar]
  12. Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH et al. Manual of Clinical Microbiology (6th edn) 3 Trends in microbiology; 1995 p 449
    [Google Scholar]
  13. Clinical and Laboratory Standards Institute Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard—CLSI document M44-A01 Wayne, PA: Clinical and Laboratory Standards Institute; 2009
    [Google Scholar]
  14. Mahrach Y, Mourabit N, Arakrak A, Bakkali M, Laglaoui A. Phenotypic and molecular study of carbapenemase-producing Enterobacteriaceae in a regional hospital in northern Morocco. J Clin Med Sci. 2019; 3: 113
    [Google Scholar]
  15. Ramesh N, Prasanth M, Patel A et al. Screening of CTX-M type extended spectrum beta-lactamases producing clinical isolates from Tamil Nadu, India. Research Journal of Pharmaceutical, Biological and Chemical Sciences 2015; 6: 658
    [Google Scholar]
  16. Vaidya VK. Horizontal transfer of antimicrobial resistance by extended-spectrum β lactamase-producing Enterobacteriaceae . J Lab Physicians 2011; 3: 037 042 [CrossRef] [PubMed]
    [Google Scholar]
  17. Manohar P, Shanthini T, Ayyanar R, Bozdogan B, Wilson A et al. The distribution of carbapenem- and colistin-resistance in gram-negative bacteria from the Tamil Nadu region in India. J Med Microbiol 2017a; 66: 874 883 [CrossRef] [PubMed]
    [Google Scholar]
  18. Manohar P, Shanthini T, Marathe N, Jadhav S, Slathia S et al. Genetic characteristics of plasmid-mediated extended-spectrum ß-lactamases (CTX-M) and its coexistencewith carbapenemases (NDM-1) in clinical gram negative bacteria. Indian Journla of Biotechnology 2017; 16: 189 194
    [Google Scholar]
  19. Nachimuthu R, Subramani R, Maray S, Gothandam KM, Sivamangala K et al. Characterization of carbapenem-resistant gram-negative bacteria from Tamil Nadu. J Chemother 2016; 28: 371 374 [CrossRef] [PubMed]
    [Google Scholar]
  20. Sarker JN, Ahmed SM, Sultana H, Anwar S, Tarafder S et al. Antibiogram of extended-spectrum β-lactamase (ESBL) producing Escherichia coli. Open Journal of Medical Microbiology 2019; 9: 41
    [Google Scholar]
  21. Pavez M, Troncoso C, Osses I, Salazar R, Illesca V et al. High prevalence of CTX-M-1 group in ESBL-producing Enterobacteriaceae infection in intensive care units in southern Chile. Braz J Infect Dis 2019; 23: 102 110 [CrossRef] [PubMed]
    [Google Scholar]
  22. Bevan ER, Jones AM, Hawkey PM. Global epidemiology of CTX-M β-lactamases: temporal and geographical shifts in genotype. J Antimicrob Chemother 2017; 72: 2145 2155 [CrossRef] [PubMed]
    [Google Scholar]
  23. Metri BC, Jyothi P, Peerapur BV. The prevalence of ESBL among Enterobacteriaceae in a tertiary care hospital of North Karnataka, India. Journal of Clinical and Diagnostic Research 2011; 5: 470 475
    [Google Scholar]
  24. Muraleetharan M, Viswanathan T. Genotyping and molecular characterization of extended-spectrum beta-lactamases-producing uropathogenic Escherichia coli in and around Coimbatore district, Tamil Nadu, India. Urological Science 2019; 30: 244
    [Google Scholar]
  25. Kiratisin P, Apisarnthanarak A, Laesripa C, Saifon P. Molecular characterization and epidemiology of extended-spectrum-beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates causing health care-associated infection in Thailand, where the CTX-M family is endemic. Antimicrob Agents Chemother 2008; 52: 2818 2824 [CrossRef] [PubMed]
    [Google Scholar]
  26. Shahid M, Malik A, Bhargava R. Increasing secondary bacterial infections with Enterobacteriaceae harboring bla(CTX-M-15) and bla(CMY-6) in patients with bronchogenic carcinoma: an emerging point of concern. Asian Pac J Trop Med 2011; 4: 5 12 [CrossRef] [PubMed]
    [Google Scholar]
  27. S S, Reddy A SK, S S, C A, V S, Senthamarai S et al. Resistance pattern of Pseudomonas aeruginosa in a tertiary care hospital of Kanchipuram, Tamilnadu, India. J Clin Diagn Res 2014; 8: DC30 [CrossRef] [PubMed]
    [Google Scholar]
  28. Haque SF, Ali S-Z, TP M, Khan AU, Mohammed TP et al. Prevalence of plasmid mediated blaTEM-1 and blaCTX-M-15 type extended spectrum beta–lactamases in patients with sepsis. Asian Pac J Trop Med 2012; 5: 98 102 [CrossRef]
    [Google Scholar]
  29. Olesen B, Hansen DS, Nilsson F et al. Prevalence and characteristics of the epidemic multi-resistant Escherichia coli ST131 clonal group among extended-spectrum β-lactamase (ESBL)-producing E. coli in Copenhagen. J Clin Microbiol 2013 JCM-00346.
    [Google Scholar]
  30. Shi H, Sun F, Chen J, Ou Q, Feng W et al. Epidemiology of CTX-M-type extended-spectrum beta-lactamase (ESBL)-producing nosocomial -Escherichia coli infection in China. Ann Clin Microbiol Antimicrob 2015; 14: 4 [CrossRef] [PubMed]
    [Google Scholar]
  31. Nagarajan P, Kennedy A, Antony A et al. Genetic determination and characterization of extended spectrum β-lactamase producing Escherichia coli and Klebsiella pneumoniae in a tertiary care Hospital, India. Indian Journal of Biotechnology 2019; 18:
    [Google Scholar]
  32. Silva-Sanchez J, Garza-Ramos JU, Reyna-Flores F, Sánchez-Perez A, Rojas-Moreno T et al. Extended-Spectrum β-lactamase-producing Enterobacteriaceae causing nosocomial infections in Mexico. A retrospective and multicenter study. Arch Med Res 2011; 42: 156 162 [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/acmi/10.1099/acmi.0.000142
Loading
/content/journal/acmi/10.1099/acmi.0.000142
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error