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Volume 2, Issue 8

Whatman FTA cards versus plasma specimens for the quantitation of HIV-1 RNA using two real-time PCR assays Open Access

Abstract

Several studies have compared the use of dried blot spot (DBS) as an alternative to plasma specimens, mainly using Whatman 903 cards as filter paper. The aim of this study was to evaluate the use of Whatman FTA card (FTA card) specimens for HIV-1 viral load testing compared to plasma specimens using two real-time PCR assays manufactured by Roche and Abbott.

A cross-sectional study was conducted between April 2017 and September 2017 on HIV-1 patients admitted to Yalgado Ouédraogo Teaching Hospital. Paired FTA cards and plasma specimens were collected and analysed using the Abbott Real-Time HIV-1 assay (Abbott) and COBAS AmpliPrep/COBAS TaqMan v2.0 (Roche).

In total, 107 patients were included. No statistical differences (>0.05) were observed between the mean viral loads obtained from the FTA cards and those of the plasma specimens using the Roche and Abbott assays. In total, 29 samples with Roche and 15 samples with Abbott assay showed discrepant results. At viral loads of ≤1000 copies ml, the sensitivity and specificity of the FTA cards were 78.6 and 100% with Roche, and 92.3 and 95.9% with Abbott, respectively. Both the Roche and Abbott assays showed good correlation and agreement between the FTA cards and plasma values.

Our study demonstrates the feasibility of using FTA card filter paper for HIV-1 viral load testing. However, further studies will be required for the validation of the use of FTA card filter paper in HIV-1 treatment monitoring.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Burkina Faso , dried blood spots , HIV-1 , real-time PCR , viral load and Whatman FTA cards
© 2020 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2020-06-17
2024-04-19
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References

  1. World Health Organization Antiretroviral therapy for HIV infection in adults and adolescents: recommendations for a public health approach - 2010 revision, 2010 rev. World Health Organization. https://apps.who.int/iris/handle/10665/44379. (accessed August 26, 2019).
  2. O'Connor J, Vjecha MJ, Phillips AN, Angus B, Cooper D et al. Effect of immediate initiation of antiretroviral therapy on risk of severe bacterial infections in HIV-positive people with CD4 cell counts of more than 500 cells per μL: secondary outcome results from a randomised controlled trial. Lancet HIV 2017; 4:e105–e112 [View Article][PubMed]
     [Google Scholar]
  3. Hamers RL, Wallis CL, Kityo C, Siwale M, Mandaliya K et al. Hiv-1 drug resistance in antiretroviral-naive individuals in sub-Saharan Africa after rollout of antiretroviral therapy: a multicentre observational study. Lancet Infect Dis 2011; 11:750–759 [View Article][PubMed]
     [Google Scholar]
  4. Barnabas RV, Revill P, Tan N, Phillips A. Cost-Effectiveness of routine viral load monitoring in low- and middle-income countries: a systematic review. J Int AIDS Soc 2017; 20 Suppl 7:e25006 [View Article][PubMed]
     [Google Scholar]
  5. Ginocchio CC, Wang XP, Kaplan MH, Mulligan G, Witt D et al. Effects of specimen collection, processing, and storage conditions on stability of human immunodeficiency virus type 1 RNA levels in plasma. J Clin Microbiol 1997; 35:2886–2893 [View Article][PubMed]
     [Google Scholar]
  6. Bonner K, Siemieniuk RA, Boozary A, Roberts T, Fajardo E et al. Expanding access to HIV viral load testing: a systematic review of RNA stability in EDTA tubes and PPT beyond current time and temperature thresholds. PLoS One 2014; 9:e113813 [View Article][PubMed]
     [Google Scholar]
  7. Hardie D, Korsman S, Ameer S, Vojnov L, Hsiao N-Y. Reliability of plasma HIV viral load testing beyond 24 hours: insights gained from a study in a routine diagnostic laboratory. PLoS One 2019; 14:e0219381 [View Article][PubMed]
     [Google Scholar]
  8. World Health Organization Consolidated guidelines on the use of antiretroviral drugs for treating and preventing HIV infection n.d.
  9. World Health Organization Consolidated guidelines on the use of antiretroviral drugs for treating and preventing HIV infection n.d.
  10. Singh D, Dhummakupt A, Siems L, Persaud D. Alternative sample types for HIV-1 antiretroviral drug resistance testing. J Infect Dis 2017; 216:S834–S837 [View Article][PubMed]
     [Google Scholar]
  11. Zhang G, DeVos J, Medina-Moreno S, Wagar N, Diallo K et al. Utilization of dried blood spot specimens can expedite nationwide surveillance of HIV drug resistance in resource-limited settings. PLoS One 2018; 13:e0203296 [View Article][PubMed]
     [Google Scholar]
  12. Viljoen J, Gampini S, Danaviah S, Valéa D, Pillay S et al. Dried blood spot HIV-1 RNA quantification using open real-time systems in South Africa and Burkina Faso. J Acquir Immune Defic Syndr 2010; 55:290–298 [View Article][PubMed]
     [Google Scholar]
  13. Reigadas S, Schrive MH, Aurillac-Lavignolle V, Fleury HJ. Quantitation of HIV-1 RNA in dried blood and plasma spots. J Virol Methods 2009; 161:177–180 [View Article][PubMed]
     [Google Scholar]
  14. Arredondo M, Garrido C, Parkin N, Zahonero N, Bertagnolio S et al. Comparison of HIV-1 RNA measurements obtained by using plasma and dried blood spots in the automated Abbott real-time viral load assay. J Clin Microbiol 2012; 50:569–572 [View Article][PubMed]
     [Google Scholar]
  15. Zeh C, Ndiege K, Inzaule S, Achieng R, Williamson J et al. Evaluation of the performance of Abbott m2000 and Roche Cobas AmpliPrep/Cobas TaqMan assays for HIV-1 viral load determination using dried blood spots and dried plasma spots in Kenya. PLoS One 2017; 12:e0179316 [View Article][PubMed]
     [Google Scholar]
  16. Rutstein SE, Hosseinipour MC, Kamwendo D, Soko A, Mkandawire M et al. Dried blood spots for viral load monitoring in Malawi: feasible and effective. PLoS One 2015; 10:e0124748 [View Article][PubMed]
     [Google Scholar]
  17. Smit PW, Sollis KA, Fiscus S, Ford N, Vitoria M et al. Systematic review of the use of dried blood spots for monitoring HIV viral load and for early infant diagnosis. PLoS One 2014; 9:e86461 [View Article][PubMed]
     [Google Scholar]
  18. Ghai RR, Thurber MI, El Bakry A, Chapman CA, Goldberg TL. Multi-method assessment of patients with febrile illness reveals over-diagnosis of malaria in rural Uganda. Malar J 2016; 15:460 [View Article][PubMed]
     [Google Scholar]
  19. Vidya M, Saravanan S, Rifkin S, Solomon SS, Waldrop G et al. Dried blood spots versus plasma for the quantitation of HIV-1 RNA using a real-time PCR, m2000rt assay. J Virol Methods 2012; 181:177–181 [View Article][PubMed]
     [Google Scholar]
  20. Mbida AD, Sosso S, Flori P, Saoudin H, Lawrence P et al. Measure of viral load by using the Abbott real-time HIV-1 assay on dried blood and plasma spot specimens collected in 2 rural dispensaries in Cameroon. J Acquir Immune Defic Syndr 2009; 52:9–16 [View Article][PubMed]
     [Google Scholar]
  21. Ouma KN, Basavaraju SV, Okonji JA, Williamson J, Thomas TK et al. Evaluation of quantification of HIV-1 RNA viral load in plasma and dried blood spots by use of the semiautomated COBAS AMPLICOR assay and the fully automated COBAS Ampliprep/TaqMan assay, version 2.0, in Kisumu, Kenya. J Clin Microbiol 2013; 51:1208–1218 [View Article][PubMed]
     [Google Scholar]
  22. Waters L, Kambugu A, Tibenderana H, Meya D, John L et al. Evaluation of filter paper transfer of whole-blood and plasma samples for quantifying HIV RNA in subjects on antiretroviral therapy in Uganda. J Acquir Immune Defic Syndr 2007; 46:590–593 [View Article][PubMed]
     [Google Scholar]
  23. Monleau M, Montavon C, Laurent C, Segondy M, Montes B et al. Evaluation of different RNA extraction methods and storage conditions of dried plasma or blood spots for human immunodeficiency virus type 1 RNA quantification and PCR amplification for drug resistance testing. J Clin Microbiol 2009; 47:1107–1118 [View Article][PubMed]
     [Google Scholar]
  24. Marconi A, Balestrieri M, Comastri G, Pulvirenti FR, Gennari W et al. Evaluation of the Abbott real-time HIV-1 quantitative assay with dried blood spot specimens. Clin Microbiol Infect 2009; 15:93–97 [View Article][PubMed]
     [Google Scholar]
  25. Leelawiwat W, Young NL, Chaowanachan T, Ou CY, Culnane M et al. Dried blood spots for the diagnosis and quantitation of HIV-1: stability studies and evaluation of sensitivity and specificity for the diagnosis of infant HIV-1 infection in Thailand. J Virol Methods 2009; 155:109–117 [View Article][PubMed]
     [Google Scholar]
  26. Nyagupe C, Shewade HD, Ade S, Timire C, Tweya H et al. HIV Viral Load Estimation Using Hematocrit Corrected Dried Blood Spot Results on a BioMerieux NucliSENS® Platform. Diagnostics 2019; 9:86 [View Article][PubMed]
     [Google Scholar]
  27. Guichet E, Serrano L, Laurent C, Eymard-Duvernay S, Kuaban C et al. Comparison of different nucleic acid preparation methods to improve specific HIV-1 RNA isolation for viral load testing on dried blood spots. J Virol Methods 2018; 251:75–79 [View Article][PubMed]
     [Google Scholar]
  28. Seu L, Mwape I, Guffey MB. Single genome amplification of proviral HIV-1 DNA from dried blood spot specimens collected during early infant screening programs in Lusaka, Zambia. J Virol Methods 2014; 203:97–101 [View Article][PubMed]
     [Google Scholar]
  29. Andreotti M, Pirillo M, Guidotti G, Ceffa S, Paturzo G et al. Correlation between HIV-1 viral load quantification in plasma, dried blood spots, and dried plasma spots using the Roche COBAS TaqMan assay. J Clin Virol 2010; 47:4–7 [View Article][PubMed]
     [Google Scholar]
  30. Taieb F, Tram TH, Ho HT, Pham VA, Nguyen L et al. Evaluation of two techniques for viral load monitoring using dried blood spot in routine practice in Vietnam (French national agency for AIDS and hepatitis research 12338). Open Forum Infect Dis 2016; 3:ofw142 [View Article][PubMed]
     [Google Scholar]
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Whatman FTA cards versus plasma specimens for the quantitation of HIV-1 RNA using two real-time PCR assays
Access Microbiology 2, e000138 (2020); https://doi.org/10.1099/acmi.0.000138
/content/journal/acmi/10.1099/acmi.0.000138
/content/journal/acmi/10.1099/acmi.0.000138
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