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Abstract

The diagnosis of tuberculous meningitis (TBM) is a major global health concern due to its protean nature. There is a need to identify better biomarkers for the rapid and definitive diagnosis of TBM. Lipids have been poorly explored as diagnostic markers in TBM.

Non-polar lipids (NPL) and mycobacterial sonicate extract (MTSE) antigens were assessed for diagnosis of .

A total of 110 cerebrospinal fluid samples were categorized as confirmed, suspected and non-TBM cases according to clinical presentation and laboratory investigations, which were further analysed by NPL and MTSE ELISA.

The sensitivity and specificity of the NPL ELISA were 39.6 and 96 %, respectively, whereas the MTSE ELISA was 17 % sensitive and 92 % specific. The combination of the NPL and MTSE ELISA test was superior to these tests alone, with sensitivity and specificity of 43 and 88 %, respectively.

This combination may be useful as an adjunct in the laboratory diagnosis of TBM. However, future studies in different settings among different populations, such as those with human immunodeficiency virus co-infection, are desirable to explore the full potential of biomarkers.

Funding
This study was supported by the:
  • UGC-DAE Consortium for Scientific Research, University Grants Commission (Award No. 20-06/2010(i)EU-IV)
    • Principle Award Recipient: Kavitha Kumar
  • Council of Scientific and Industrial Research, India (Award No. 09/490(0083)/2012)
    • Principle Award Recipient: Prashant Giribhattanavar
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2020-05-11
2024-03-29
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