A case of canine cutaneous pythiosis in Thailand Open Access

Abstract

causes pythiosis in humans and animals in tropical and subtropical climates. The clinical manifestations in humans are mostly systemic, vascular or ocular forms, in contrast to animals, which are cutaneous, subcutaneous and gastrointestinal forms. The highest incidence of human cases is reported in Thailand, however, no canine pythiosis has been documented yet.

A female, mixed-breed, stray dog showed severe extensive ulcerative haemorrhagic dermatitis at the perineum involving the anus and tail. On cytology, there were sparse branching septate fungal hyphae. The tissue samples were subjected to polymerase chain reaction and gene sequencing for fungal identification.

The results of the internal transcribed spacer 1 and 2 (ITS1 and ITS2) gene had 99 % homology to (accession no. FJ17396) and the gene (accession no. GQ451572). The phylogenetic tree of both genes was classified in clade A This is the first fully documented diagnosis of canine cutaneous pythiosis in Thailand.

Loading

Article metrics loading...

/content/journal/acmi/10.1099/acmi.0.000109
2020-02-14
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/acmi/2/4/acmi000109.html?itemId=/content/journal/acmi/10.1099/acmi.0.000109&mimeType=html&fmt=ahah

References

  1. Chaffin MK, James S, McMullan WC. Cutaneous pythiosis in the horse. Vet Clin North Am Equine Pract 1995; 11:91–103 [View Article]
    [Google Scholar]
  2. Gaastra W, Lipman LJA, De Cock AWAM, Exel TK, Pegge RBG et al. Pythium insidiosum: an overview. Vet Microbiol 2010; 146:1–16 [View Article]
    [Google Scholar]
  3. Maia LA, Olinda RG, Araújo TF, Firmino PR, Nakazato L et al. Cutaneous pythiosis in a donkey (Equus asinus) in Brazil. J VET Diagn Invest 2016; 28:436–439 [View Article]
    [Google Scholar]
  4. Mercer J, White A, Kennis B. Successful management of cutaneous pythiosis in a dog with subsequent cutaneous vasculitis. Vet Rec Case Rep 2014; 2:e000143 [View Article]
    [Google Scholar]
  5. Neto RT, de M.G. Bosco S, Amorim RL, Brandão CVS, Fabris VE et al. Cutaneous pythiosis in a dog from Brazil. Vet Dermatol 2010; 21:202–204 [View Article]
    [Google Scholar]
  6. Oldenhoff W, Grooters A, Pinkerton ME, Knorr J, Trepanier L. Cutaneous pythiosis in two dogs from Wisconsin, USA. Vet Dermatol 2014; 25:52–e21 [View Article]
    [Google Scholar]
  7. Pesavento PA, Barr B, Riggs SM, Eigenheer AL, Pamma R et al. Cutaneous pythiosis in a nestling white-faced IBIS. Vet Pathol 2008; 45:538–541 [View Article]
    [Google Scholar]
  8. Schurko A, Mendoza L, de Cock AWAM, Klassen GR. Evidence for geographic clusters: Molecular genetic differences among strains of Pythium insidiosum from Asia, Australia and the Americas are explored. Mycologia 2003; 95:200–208 [View Article]
    [Google Scholar]
  9. Rivierre C, Laprie C, Guiard-Marigny O, Bergeaud P, Berthelemy M et al. Pythiosis in Africa. Emerg Infect Dis 2005; 11:479–481 [View Article]
    [Google Scholar]
  10. Dykstra MJ, Sharp NJ, Olivry T, Hillier A, Murphy KM et al. A description of cutaneous-subcutaneous pythiosis in fifteen dogs. Med Mycol 1999; 37:427–433 [View Article]
    [Google Scholar]
  11. De Cock AW, Mendoza L, Padhye AA, Ajello L, Kaufman L. Pythium insidiosum sp. nov., the etiologic agent of pythiosis. J Clin Microbiol 1987; 25:344–349 [View Article]
    [Google Scholar]
  12. Kepler D, Cole R, Lee-Fowler T, Koehler J, Shrader S et al. Pulmonary pythiosis in a canine patient. Vet Radiol Ultrasound 2019; 60:E20–E23 [View Article]
    [Google Scholar]
  13. Permpalung N, Worasilchai N, Chindamporn A. Human pythiosis: emergence of Fungal-Like organism. Mycopathologia 2019 [View Article]
    [Google Scholar]
  14. Kammarnjesadakul P, Palaga T, Sritunyalucksana K, Mendoza L, Krajaejun T et al. Phylogenetic analysis of Pythium insidiosum Thai strains using cytochrome oxidase II (COX II) DNA coding sequences and internal transcribed spacer regions (ITS). Med Mycol 2011; 49:289–295 [View Article]
    [Google Scholar]
  15. Vilela R, Montalva C, Luz C, Humber RA, Mendoza L. Pythium insidiosum isolated from infected mosquito larvae in central Brazil. Acta Trop 2018; 185:344–348 [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/acmi/10.1099/acmi.0.000109
Loading
/content/journal/acmi/10.1099/acmi.0.000109
Loading

Data & Media loading...

Most cited Most Cited RSS feed