%0 Journal Article %A Ragupathi, Naveen Kumar Devanga %A Sethuvel, Dhiviya Prabaa Muthuirulandi %A Anandan, Shalini %A Murugan, Dhivya %A Asokan, Kalaiarasi %A Neethi Mohan, Ramya Gajaraj %A Vasudevan, Karthick %A D, Thirumal Kumar %A C, George Priya Doss %A Veeraraghavan, Balaji %T First hybrid complete genome of Aeromonas veronii reveals chromosome-mediated novel structural variant mcr-3.30 from a human clinical sample %D 2020 %J Access Microbiology, %V 2 %N 4 %@ 2516-8290 %C e000103 %R https://doi.org/10.1099/acmi.0.000103 %K mcr-3 %K blaOXA-12 %K Aeromonas %K blaCEPH-A3 %K ISAs18 %K colistin %I Microbiology Society, %X Recent findings demonstrate the origin of the plasmid-mediated colistin resistance gene mcr-3 from aeromonads. The present study aimed to screen for plasmid-mediated colistin resistance among 30 clinical multidrug-resistant (MDR) Aeromonas spp. PCR was used to screen for the presence of mcr-1, mcr-2, mcr-3 and mcr-4, which revealed mcr-3 in a colistin-susceptible isolate (FC951). All other isolates were negative for mcr. Sequencing of FC951 revealed that the mcr-3 (mcr-3.30) identified was different from previously reported variants and had 95.62 and 95.28 % nucleotide similarity with mcr-3.3 and mcr-3.10. Hybrid assembly using IonTorrent and MinION reads revealed structural genetic information for mcr-3.30 with an insertion of ISAs18 within the gene. Due to this, mcr-3.30 was non-expressive, which makes FC951 susceptible to colistin. Further, in silico sequence and protein structural analysis confirmed the new variant. To the best of our knowledge, this is the first report on a novel mcr-3 variant from India. The significant role of mcr-like genes in different Aeromonas species remains unknown and requires additional investigation to obtains insights into the mechanism of colistin resistance. %U https://www.microbiologyresearch.org/content/journal/acmi/10.1099/acmi.0.000103