1887

Abstract

Recent findings demonstrate the origin of the plasmid-mediated colistin resistance gene from aeromonads. The present study aimed to screen for plasmid-mediated colistin resistance among 30 clinical multidrug-resistant (MDR) spp. PCR was used to screen for the presence of , , and , which revealed in a colistin-susceptible isolate (FC951). All other isolates were negative for . Sequencing of FC951 revealed that the () identified was different from previously reported variants and had 95.62 and 95.28 % nucleotide similarity with and . Hybrid assembly using IonTorrent and MinION reads revealed structural genetic information for with an insertion of IS within the gene. Due to this, was non-expressive, which makes FC951 susceptible to colistin. Further, sequence and protein structural analysis confirmed the new variant. To the best of our knowledge, this is the first report on a novel variant from India. The significant role of -like genes in different species remains unknown and requires additional investigation to obtains insights into the mechanism of colistin resistance.

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2020-02-17
2020-06-02
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