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Abstract

Dengue is an important vector borne disease with a great public health concern worldwide. Northeast India has experienced dengue almost every year for a decade. As studies on dengue vectors from this region are limited, we undertook an investigation to detect natural infection of the dengue virus (DENV) in potential dengue vectors of this region. Adult mosquitoes which were collected were subjected to RT-PCR for detection of infecting dengue serotype. Minimum infection rate was also determined for each positive pool. Out of the total 6229 adult mosquitoes collected, (63.3 %) was abundant in comparison to (36.7 %). These specimens (515 mosquito pools) were subjected to RT-PCR for detection of DENV-1, 2, 3 and 4. RT-PCR revealed the existence of DENV in both male as well as female mosquito pools suggesting natural transovarial transmission of DENV in this region. A total of 54 pools tested were positive for DENV-1, 2, 3 serotypes. This study revealed the occurence of DENV in both the potential dengue vectors from this region along with evidence of transovarial transmission which helps in persistence of the virus in nature.

Funding
This study was supported by the:
  • Indian Council of Medical Research (Award Ref. No. VIR/9/2017/ECD-1 dated 21.08.17)
    • Principle Award Recipient: N. Pradeep Kumar
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2020-03-26
2024-04-19
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