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Abstract

Dengue is an important vector borne disease with a great public health concern worldwide. Northeast India has experienced dengue almost every year for a decade. As studies on dengue vectors from this region are limited, we undertook an investigation to detect natural infection of the dengue virus (DENV) in potential dengue vectors of this region. Adult mosquitoes which were collected were subjected to RT-PCR for detection of infecting dengue serotype. Minimum infection rate was also determined for each positive pool. Out of the total 6229 adult mosquitoes collected, (63.3 %) was abundant in comparison to (36.7 %). These specimens (515 mosquito pools) were subjected to RT-PCR for detection of DENV-1, 2, 3 and 4. RT-PCR revealed the existence of DENV in both male as well as female mosquito pools suggesting natural transovarial transmission of DENV in this region. A total of 54 pools tested were positive for DENV-1, 2, 3 serotypes. This study revealed the occurence of DENV in both the potential dengue vectors from this region along with evidence of transovarial transmission which helps in persistence of the virus in nature.

Funding
This study was supported by the:
  • N. Pradeep Kumar , Indian Council of Medical Research , (Award Ref. No. VIR/9/2017/ECD-1 dated 21.08.17)
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2020-03-26
2020-03-31
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References

  1. Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW et al. The global distribution and burden of dengue. Nature 2013; 496: 504 507 [CrossRef] [PubMed]
    [Google Scholar]
  2. Chetry S, Khan SA, Dutta P, Apum B, Medhi PS et al. Dengue virus serotypes and genotypic characterization from Northeast India. J Med Virol 2019; 91: 918 927 [CrossRef] [PubMed]
    [Google Scholar]
  3. Lee HL, Rohani A. Transovarial transmission of dengue virus in Aedes aegypti and Aedes albopictus in relation to dengue outbreak in an urban area in Malaysia Dengue Bulletin; 2015
    [Google Scholar]
  4. Chow VT, Chan YC, Yong R, Lee KM, Lim LK et al. Monitoring of dengue viruses in field-caught Aedes aegypti and Aedes albopictus mosquitoes by a type-specific polymerase chain reaction and cycle sequencing. Am J Trop Med Hyg 1998; 58: 578 586 [CrossRef] [PubMed]
    [Google Scholar]
  5. Gupta N, Srivastava S, Jain A, Chaturvedi UC. Dengue in India. Indian J Med Res 2012; 136: 373 390 [PubMed]
    [Google Scholar]
  6. Joshi V, Singhi M, Chaudhary RC. Transovarial transmission of dengue 3 virus by Aedes aegypti. Trans R Soc Trop Med Hyg 1996; 90: 643 644 [CrossRef] [PubMed]
    [Google Scholar]
  7. Rodhain F, Rosen L. Mosquito Vectors and Dengue Virus Vector Relationships New York: CAB International; 1997
    [Google Scholar]
  8. Mourya DT, Gokhale BA, Basu A, Barde PV, Sapkal GN et al. Horizontal and vertical transmission of dengue virus type 2 in highly and lowly susceptible strains of Aedes aegypti mosquitoes. Acta Virol 2001; 45: 67 71 [PubMed]
    [Google Scholar]
  9. Dutta P, Khan SA, Khan AM, Sharma CK, Mahanta J. An updated checklist of species of Aedes and Verrallina of northeastern India. J Am Mosq Control Assoc 2010; 26: 135 140 [CrossRef] [PubMed]
    [Google Scholar]
  10. Dutta P, Khan SA, Chetry S, Dev V, Sarmah CK et al. First evidence of dengue virus infection in wild caught mosquitoes during an outbreak in Assam, Northeast India. J Vector Borne Dis 2015; 52: 293 298 [PubMed]
    [Google Scholar]
  11. Dutta P, Khan SA, Chetry S, Abdul M, Khan AM. Incrimination of Aedes aegypti for dengue virus serotype-1 in Assam, Northeast India. J Vector Borne Dis 2018; 55: 330 333 [CrossRef] [PubMed]
    [Google Scholar]
  12. Barraud PJ. The Fauna of British India, Including Ceylon and Burma. Diptera. Volume V. Family Culicidae. Tribes Megarhinini and Culicini London: Taylor and Francis; 1934
    [Google Scholar]
  13. Chomczynski P. A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques 1993; 15: 532-534 532-536 [PubMed]
    [Google Scholar]
  14. Molecular Research Center, Inc. Cincinnati, OH TRI Reagent - RNA, DNA, protein isolation reagent. Manufacturer's protocol (1995). https://search.cosmobio.co.jp/cosmo_search_p/search_gate2/docs/MOR_/TR118.20100414.pdf
  15. Lanciotti RS, Calisher CH, Gubler DJ, Chang GJ, Vorndam AV. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J Clin Microbiol 1992; 30: 545 551 [CrossRef] [PubMed]
    [Google Scholar]
  16. Harris E, Roberts TG, Smith L, Selle J, Kramer LD et al. Typing of dengue viruses in clinical specimens and mosquitoes by single-tube multiplex reverse transcriptase PCR. J Clin Microbiol 1998; 36: 2634 2639 [CrossRef] [PubMed]
    [Google Scholar]
  17. Thongrungkiat S, Maneekan P, Wasinpiyamongkol L, Prummongkol S. Prospective field study of transovarial dengue-virus transmission by two different forms of Aedes aegypti in an urban area of Bangkok, Thailand. J Vector Ecol 2011; 36: 147 152 [CrossRef] [PubMed]
    [Google Scholar]
  18. Gu W, Lampman R, Novak RJ. Assessment of arbovirus vector infection rates using variable size pooling. Med Vet Entomol 2004; 18: 200 204 [CrossRef] [PubMed]
    [Google Scholar]
  19. Mavale M, Parashar D, Sudeep A, Gokhale M, Ghodke Y et al. Venereal transmission of Chikungunya virus by Aedes aegypti mosquitoes (Diptera: Culicidae). Am J Trop Med Hyg 2010; 83: 1242 1244 [CrossRef] [PubMed]
    [Google Scholar]
  20. Cecílio AB, Campanelli ES, Souza KPR, Figueiredo LB, Resende MC. Natural vertical transmission by Stegomyia albopicta as dengue vector in Brazil. Braz J Biol 2009; 69: 123 127 [CrossRef] [PubMed]
    [Google Scholar]
  21. Ganeshkumar P, Murhekar MV, Poornima V, Saravanakumar V, Sukumaran K et al. Dengue infection in India: a systematic review and meta-analysis. PLoS Negl Trop Dis 2018; 12: e0006618 [CrossRef] [PubMed]
    [Google Scholar]
  22. Chakravarti A, Arora R, Luxemburger C. Fifty years of dengue in India. Trans R Soc Trop Med Hyg 2012; 106: 273 282 [CrossRef] [PubMed]
    [Google Scholar]
  23. Halstead SB. Dengue. Lancet 2007; 370: 1644 1652 [CrossRef] [PubMed]
    [Google Scholar]
  24. Victor TJ. Detection of dengue viral infections in Aedes mosquitoes: an essential tool for epidemiological surveillance. Indian J Med Res 2009; 129: 634 636 [PubMed]
    [Google Scholar]
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