Oral flora meningoencephalitis diagnosis by next-generation DNA sequencing Open Access

Abstract

. Standard culture methods may fail to detect the causative agents of bacterial infection for various reasons including specimen collection after antibiotic administration, or when standard techniques or environmental conditions are not appropriate for growth of the microorganisms. Conventional 16S rRNA gene sequencing is sometimes a useful alternative technique for identification of bacteria, but is confounded by polymicrobial infection. We present a case of a patient who developed a serious neurological infection for which causative oral flora organisms were observed by microscopy, failed to culture but were identified by next-generation DNA sequencing.

A male in his forties developed sinus pain and congestion, followed by facial and eye pain, and several weeks later acute-onset confusion and neck stiffness. Cerebrospinal fluid examination revealed pleocytosis and several bacterial morphologies, which were subsequently identified by next-generation sequencing as oral flora constituents , , , species and .

Oral flora can cause meningoencephalitis and brain abscess formation if translocation occurs by injury or surgical procedures. Next-generation sequencing is often not available at healthcare facilities, or when available may not have been validated for a wide spectrum of specimen sources, but is available at reference laboratories and should be considered when routine methods fail to provide a diagnosis for serious infections.

Loading

Article metrics loading...

/content/journal/acmi/10.1099/acmi.0.000056
2019-11-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/acmi/1/9/acmi000056.html?itemId=/content/journal/acmi/10.1099/acmi.0.000056&mimeType=html&fmt=ahah

References

  1. Thigpen MC, Whitney CG, Messonnier NE, Zell ER, Lynfield R et al. Bacterial meningitis in the United States, 1998-2007. N Engl J Med 2011; 364:2016–2025 [View Article]
    [Google Scholar]
  2. Castelblanco RL, Lee M, Hasbun R. Epidemiology of bacterial meningitis in the USA from 1997 to 2010: a population-based observational study. Lancet Infect Dis 2014; 14:813–819 [View Article]
    [Google Scholar]
  3. New DW. Meningitis caused by Porphyromonas endodontalis detected by PCR amplification and sequencing of 16S rRNA genes direct from cerebrospinal fluid and cerebral tissue JMM Case Reports; 2015
  4. Keller PM, Rampini SK, Bloemberg GV. Detection of a mixed infection in a culture-negative brain abscess by broad-spectrum bacterial 16S rRNA gene PCR. J Clin Microbiol 2010; 48:2250–2252 [View Article]
    [Google Scholar]
  5. Ko J-H, Baek JY, Kang C-I, Lee WJ, Lee JY et al. Bacteremic meningitis caused by Parvimonas micra in an immunocompetent host. Anaerobe 2015; 34:161–163 [View Article]
    [Google Scholar]
  6. Veldhoen ES, Wolfs TF, van Vught AJ. Two cases of fatal meningitis due to Fusobacterium necrophorum. Pediatr Neurol 2007; 36:261–263 [View Article]
    [Google Scholar]
  7. Roca B, Romero A, Simón E. Acute meningitis caused by Streptococcus constellatus. J Infect 1998; 37:88 [View Article]
    [Google Scholar]
  8. García-Lechuz JM, Hernangómez S, San Juan R, Bouza E. Feculent meningitis: polymicrobial meningitis in colorectal surgery. Diagn Microbiol Infect Dis 2000; 38:169–170 [View Article]
    [Google Scholar]
  9. Chun C, Sacks B, Jao J. Polymicrobial feculent meningitis. Clin Infect Dis 1995; 21:693–694 [View Article]
    [Google Scholar]
  10. Salipante SJ, Hoogestraat DR, Abbott AN, SenGupta DJ, Cummings LA et al. Coinfection of Fusobacterium nucleatum and Actinomyces israelii in mastoiditis diagnosed by next-generation DNA sequencing. J Clin Microbiol 2014; 52:1789–1792 [View Article]
    [Google Scholar]
  11. Salipante SJ, Sengupta DJ, Rosenthal C, Costa G, Spangler J et al. Rapid 16S rRNA next-generation sequencing of polymicrobial clinical samples for diagnosis of complex bacterial infections. PLoS One 2013; 8:e65226 [View Article]
    [Google Scholar]
  12. Zaura E, Keijser BJF, Huse SM, Crielaard W. Defining the healthy "core microbiome" of oral microbial communities. BMC Microbiol 2009; 9:259 [View Article]
    [Google Scholar]
  13. Chen T, Tu WH, Izard J, Baranova OV, Lakshmanan A et al. The human oral microbiome database: a web accessible resource for investigating oral microbe taxonomic and genomic information. Database 20102010, baq013
    [Google Scholar]
  14. Dewhirst FE, Chen T, Izard J, Paster BJ, Tanner ACR et al. The human oral microbiome. J Bacteriol 2010; 192:5002–5017 [View Article]
    [Google Scholar]
  15. Santos AL, Siqueira JF, Rôças IN, Jesus EC, Rosado AS et al. Comparing the bacterial diversity of acute and chronic dental root canal infections. PLoS One 2011; 6:e28088 [View Article]
    [Google Scholar]
  16. Machado de Oliveira JC, Siqueira JF, Alves GB, Hirata R, Andrade AF. Detection of Porphyromonas endodontalis in infected root canals by 16S rRNA gene-directed polymerase chain reaction. J Endod 2000; 26:729–732 [View Article]
    [Google Scholar]
  17. Signat B, Roques C, Poulet P, Duffaut D. Fusobacterium nucleatum in periodontal health and disease. Curr Issues Mol Biol 2011; 13:25–36
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/acmi/10.1099/acmi.0.000056
Loading
/content/journal/acmi/10.1099/acmi.0.000056
Loading

Data & Media loading...

Most cited Most Cited RSS feed