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Abstract

The production of biogas by anaerobic digestion (AD) of organic/biological wastes has a firm place in sustainable energy production. A simple and cost-effective anaerobic jar at a laboratory scale is a prerequisite to study the microbial community involved in biomass conversion and releasing of methane gas. In this study, a simulation was carried out using a laboratory-modified anaerobic-jar-converted digester (AD1) with that of a commercial/pilot-scale anaerobic digester (AD2). Taxonomic profiling of biogas-producing communities by means of high-throughput methyl coenzyme-M reductase α-subunit (mcrA) gene amplicon sequencing provided high-resolution insights into bacterial and archaeal structures of AD assemblages and their linkages to fed substrates and process parameters. Commonly, the bacterial phyla , , and appeared to dominate biogas communities in varying abundances depending on the apparent process conditions. Key micro-organisms identified from AD were and . Specific biogas production was found to be significantly correlating to . It can be implied from this study that the metagenomic sequencing data was able to dissect the microbial community structure in the digesters. The data gathered indicates that the anaerobic-jar system could throw light on the population dynamics of the methanogens at laboratory scale and its effectiveness at large-scale production of bio-methane. The genome sequence information of non-cultivable biogas community members, metagenome sequencing including assembly and binning strategies will be highly valuable in determining the efficacy of an anaerobic digester.

  • This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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2019-07-01
2024-11-08
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References

  1. Mata-Alvarez J, Dosta J, Romero-Güiza MS, Fonoll X, Peces M et al. A critical review on anaerobic co-digestion achievements between 2010 and 2013. Renew Sustain Energy Rev 2014; 36:412–427 [View Article]
    [Google Scholar]
  2. Abendroth C, Vilanova C, Günther T, Luschnig O, Porcar M. Eubacteria and archaea communities in seven mesophile anaerobic digester plants in Germany. Biotechnol Biofuels 2015; 8:87 [View Article]
    [Google Scholar]
  3. Bialek K, Cysneiros D, O'Flaherty V. Hydrolysis, acidification and methanogenesis during low-temperature anaerobic digestion of dilute dairy wastewater in an inverted fluidised bioreactor. Appl Microbiol Biotechnol 2014; 98:87378750 [View Article]
    [Google Scholar]
  4. Kern T, Theiss J, Röske K, Rother M. Assessment of hydrogen metabolism in commercial anaerobic digesters. Appl Microbiol Biotechnol 2016; 100:46994710 [View Article]
    [Google Scholar]
  5. Guo J, Peng Y, Ni BJ, Han X, Fan L et al. Dissecting microbial community structure and methane-producing pathways of a full-scale anaerobic reactor digesting activated sludge from wastewater treatment by metagenomic sequencing. Microb Cell Fact 2015; 14:1–11 [View Article]
    [Google Scholar]
  6. Clauss M, Hummel J. Physiological adaptations of ruminants and their potential relevance for production systems. Rev Bras Zootec 2017
    [Google Scholar]
  7. Clauss M, Hume ID, Hummel J. Evolutionary adaptations of ruminants and their potential relevance for modern production systems. Animal 2010; 4:979–992 [View Article]
    [Google Scholar]
  8. Wedlock DN, Janssen PH, Leahy SC, Shu D, Buddle BM. Progress in the development of vaccines against rumen methanogens. Animal 2013; 7:244–252 [View Article]
    [Google Scholar]
  9. Thorpe A. Enteric fermentation and ruminant eructation: the role (and control?) of methane in the climate change debate. Clim Change 2009; 93:407–431 [View Article]
    [Google Scholar]
  10. Leahy SC, Kelly WJ, Ronimus RS, Wedlock N, Altermann E et al. Genome sequencing of rumen bacteria and archaea and its application to methane mitigation strategies. Animal 2013; 7:235–243 [View Article]
    [Google Scholar]
  11. Luton PE, Wayne JM, Sharp RJ, Riley PW. The mcrA gene as an alternative to 16S rRNA in the phylogenetic analysis of methanogen populations in landfill. Microbiology 2002; 148:3521–3530 [View Article]
    [Google Scholar]
  12. Bishir M. Raji HM: quantitative and qualitative analysis of biogas produced from three organic wastes. Int J Renew Energy Res 2016; 6:299–305
    [Google Scholar]
  13. Akintokun AK, Abibu WA, Oyatogun MO. Applied Environmental Research Microbial Dynamics and Biogas Production during Single and Co-digestion of Cow Dung and Rice Husk [Internet]. Appl Environ Res 2017b; 39:67–76
    [Google Scholar]
  14. Ezekoye VA, Offor PO. Effect of retention time on biogas production from poultry droppings and cassava peels. Nig J Biotech 2011; 22:53–59
    [Google Scholar]
  15. Anderson I, Ulrich LE, Lupa B, Susanti D, Porat I et al. Genomic characterization of methanomicrobiales reveals three classes of methanogens. PLoS One 2009; 4:e5797 [View Article]
    [Google Scholar]
  16. Tejerizo GT, Kim YS, Maus I, Wibberg D, Winkler A et al. Genome sequence of Methanobacterium congolense strain Buetzberg, a hydrogenotrophic, methanogenic archaeon, isolated from a mesophilic industrial-scale biogas plant utilizing bio-waste. J Biotechnol 2017; 247:15 [View Article]
    [Google Scholar]
  17. Maus I, Stantscheff R, Wibberg D, Stolze Y, Winkler A et al. Complete genome sequence of the methanogenic neotype strain Methanobacterium formicicum MFT. J Biotechnol 2014
    [Google Scholar]
  18. Manzoor S, Schnürer A, Bongcam-Rudloff E, Müller B. Complete genome sequence of Methanoculleus bourgensis strain MAB1, the syntrophic partner of mesophilic acetate-oxidising bacteria (SAOB). Stand Genomic Sci 2016; 11:1–9 [View Article]
    [Google Scholar]
  19. Ferry JG, Smith PH, Wolfe RS. Methanospirillum, a new genus of methanogenic bacteria, and characterization of Methanospirillum hungatii sp.nov. Int J Syst Bacteriol 1974; 24:465–469 [View Article]
    [Google Scholar]
  20. Martin AD. Understanding anaerobic digestion [Internet]. http://www.esauk.org/events/Alastair_D_Martin.pdf
  21. Akintokun A, Abibu W, Oyatoogun M. Isolation and characterization of microorganisms with hydrolytic profile during anaerobic digestion and biogas production of cow dung and rice husk. J Nat Sci Res 2017a; 7:2225–2921
    [Google Scholar]
  22. Hamawand I, Baillie C. Anaerobic digestion and biogas potential: simulation of lab and industrial-scale processes. Energies 2015; 8:454–474 [View Article]
    [Google Scholar]
  23. Zinder SH. Physiological ecology of methanogens BT - methanogenesis: ecology, physiology, biochemistry and genetics; in : methanogenesis: Ecology, physiology, biochemistry and genetics; 1993
  24. Krakat N, Westphal A, Schmidt S, Scherer P. Anaerobic digestion of renewable biomass: thermophilic temperature governs methanogen population dynamics. Appl Environ Microbiol 2010; 76:1842–1850 [View Article]
    [Google Scholar]
  25. Gowdaman V, Srikanth M. Carbon – science and technology. Carbon-Sci Tech 2015; 2:8–16
    [Google Scholar]
  26. Lee B, Park JG, Shin WB, Tian DJ, Jun HB. Microbial communities change in an anaerobic digestion after application of microbial electrolysis cells. Bioresour Technol 2017; 234:273–280 [View Article]
    [Google Scholar]
  27. Angelidaki I, Alves M, Bolzonella D, Borzacconi L, Campos JL et al. Defining the biomethane potential (BMP) of solid organic wastes and energy crops: a proposed protocol for batch assays. Water Sci Technol 2009; 59:927–934 [View Article]
    [Google Scholar]
  28. Ziganshin AM, Ziganshina EE, Kleinsteuber S, Nikolausz M. Comparative analysis of methanogenic communities in different laboratory-scale anaerobic Digesters. Archaea 2016; 2016:1–12 [View Article]
    [Google Scholar]
  29. Caetano-Anollés D. Caetano-Anollés G: ribosomal accretion, apriorism and the phylogenetic method: a response to Petrov and Williams. Front Genet 2015; 6:1–6
    [Google Scholar]
  30. Westerholm M, Müller B, Isaksson S, Schnürer A. Trace element and temperature effects on microbial communities and links to biogas digester performance at high ammonia levels. Biotechnol Biofuels 2015; 8:1–19 [View Article]
    [Google Scholar]
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