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Volume 1, Issue 5

Microbiome digital signature of MCR genes – an approach to study the diversity of methanogenic population in laboratory-developed and pilot-scale anaerobic digesters Open Access

Abstract

The production of biogas by anaerobic digestion (AD) of organic/biological wastes has a firm place in sustainable energy production. A simple and cost-effective anaerobic jar at a laboratory scale is a prerequisite to study the microbial community involved in biomass conversion and releasing of methane gas. In this study, a simulation was carried out using a laboratory-modified anaerobic-jar-converted digester (AD1) with that of a commercial/pilot-scale anaerobic digester (AD2). Taxonomic profiling of biogas-producing communities by means of high-throughput methyl coenzyme-M reductase α-subunit (mcrA) gene amplicon sequencing provided high-resolution insights into bacterial and archaeal structures of AD assemblages and their linkages to fed substrates and process parameters. Commonly, the bacterial phyla , , and appeared to dominate biogas communities in varying abundances depending on the apparent process conditions. Key micro-organisms identified from AD were and . Specific biogas production was found to be significantly correlating to . It can be implied from this study that the metagenomic sequencing data was able to dissect the microbial community structure in the digesters. The data gathered indicates that the anaerobic-jar system could throw light on the population dynamics of the methanogens at laboratory scale and its effectiveness at large-scale production of bio-methane. The genome sequence information of non-cultivable biogas community members, metagenome sequencing including assembly and binning strategies will be highly valuable in determining the efficacy of an anaerobic digester.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Anaerobic digester (AD) , Biogas , Cow manure , Methanocorpusculum and Methanosarcinaceae and methyl coenzyme-M reductase α-subunit (mcrA)
© 2019 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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2019-07-01
2024-04-23
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Microbiome digital signature of MCR genes – an in silico approach to study the diversity of methanogenic population in laboratory-developed and pilot-scale anaerobic digesters
Access Microbiology 1, e000044 (2019); https://doi.org/10.1099/acmi.0.000044
/content/journal/acmi/10.1099/acmi.0.000044
/content/journal/acmi/10.1099/acmi.0.000044
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