1887

Abstract

Wastewater has become a potential habitat for multi-drug-resistant bacteria. The present study aims to screen for the presence of carbapenem-resistant bacteria in sewage water samples collected from hospital and non-hospital sources. From a total of 19 sewage water samples collected, 100 carbapenem-resistant non-lactose-fermenting Gram-negative bacteria (CR-NF-GNB) were isolated using MacConkey agar cultured with 8 mg l of meropenem. On screening for beta-lactamase resistance genes (bla NDM, bla OXA-48-like, bla IMP, bla VIM and bla KPC), one isolate, Ochrobactrum intermedium , was found to carry the plasmid-borne bla OXA-48-like gene. To the best of our knowledge, we provide the first report of the rare and emerging opportunistic pathogen Ochrobactrum intermedium encoding the OXA-181 gene in its plasmid.

Loading

Article metrics loading...

/content/journal/acmi/10.1099/acmi.0.000024
2019-05-15
2019-08-23
Loading full text...

Full text loading...

/deliver/fulltext/acmi/1/3/acmi000024.html?itemId=/content/journal/acmi/10.1099/acmi.0.000024&mimeType=html&fmt=ahah

References

  1. Hrenovic J, Ganjto M, Goic-Barisic I. Carbapenem-resistant bacteria in a secondary wastewater treatment plant. Water SA 2017;43:186–191 [CrossRef]
    [Google Scholar]
  2. Hrenovic J, Ivankovic T, Ivekovic D, Repec S, Stipanicev D et al. The fate of carbapenem-resistant bacteria in a wastewater treatment plant. Water Res 2017;126:232–239 [CrossRef]
    [Google Scholar]
  3. Pendleton JN, Gorman SP, Gilmore BF. Clinical relevance of the ESKAPE pathogens. Expert Rev Anti Infect Ther 2013;11:297–308 [CrossRef]
    [Google Scholar]
  4. Manohar P, Babu S, Bozdogan B, Ramesh N. Identification of blaDIM-1 metallo-β-lactamase gene in Pseudomonas aeruginosa isolated from Tamil Nadu, India. J Glob Antimicrob Resist 2018;13:7–8 [CrossRef]
    [Google Scholar]
  5. Nachimuthu R, Subramani R, Maray S, Gothandam KM, Sivamangala K et al. Characterization of carbapenem-resistant Gram-negative bacteria from Tamil Nadu. J Chemother 2016;28:371–374 [CrossRef]
    [Google Scholar]
  6. Staradumskyte D, Paulauskas A. Non-fermentative gram-negative bacteria in drinking water. J Water Resour Prot 2014;06:114–119 [CrossRef]
    [Google Scholar]
  7. Bharucha T, Sharma D, Sharma H, Kandil H, Collier S. Ochromobactrum intermedium: an emerging opportunistic pathogen—case of recurrent bacteraemia associated with infective endocarditis in a haemodialysis patient. New Microbes New Infect 2017;15:14–15 [CrossRef]
    [Google Scholar]
  8. Jacobs DJ, Grube TJ, Flynn HW Jr, Greven CM, Pathengay A et al. Intravitreal moxifloxacin in the management of Ochrobactrum intermedium endophthalmitis due to metallic intraocular foreign body. Clin Ophthalmol 2013;7:1727–1730 [CrossRef]
    [Google Scholar]
  9. Dharne MS, Misra SP, Misra V, Dwivedi M, Patole MS et al. Isolation of urease-positive Ochrobactrum intermedium in the stomach of a non-ulcer dyspeptic patient from North India. J Microbiol Immunol Infect 2008;41:183–186
    [Google Scholar]
  10. Berglund B. Environmental dissemination of antibiotic resistance genes and correlation to anthropogenic contamination with antibiotics. Infect Ecol Epidemiol 2015;5:28564 [CrossRef]
    [Google Scholar]
  11. Hawkey PM. The growing burden of antimicrobial resistance. J Antimicrob Chemother 2008;62:i1–i9 [CrossRef]
    [Google Scholar]
  12. Kümmerer K. Resistance in the environment. J Antimicrob Chemother 2004;54:311–320 [CrossRef]
    [Google Scholar]
  13. Clinical, Institute LS Performance Standards for Antimicrobial Susceptibility Testing of Anaerobic Bacteria, Informational Supplement. Clinical and Laboratory Standards Institute (CLSI); 2009
    [Google Scholar]
  14. Manohar P, Shanthini T, Ayyanar R, Bozdogan B, Wilson A et al. The distribution of carbapenem- and colistin-resistance in Gram-negative bacteria from the Tamil Nadu region in India. J Med Microbiol 2017;66:874–883 [CrossRef]
    [Google Scholar]
  15. Lin L, Ling BD, Li XZ. Distribution of the multidrug efflux pump genes, adeABC, adeDE and adeIJK, and class 1 integron genes in multiple-antimicrobial-resistant clinical isolates of Acinetobacter baumanniiAcinetobacter calcoaceticus complex. Int J Antimicrob Agents 2009;33:27–32 [CrossRef]
    [Google Scholar]
  16. Moges F, Endris M, Belyhun Y, Worku W. Isolation and characterization of multiple drug resistance bacterial pathogens from waste water in hospital and non-hospital environments, Northwest Ethiopia. BMC Res Notes 2014;7:215 [CrossRef]
    [Google Scholar]
  17. Akiba M, Sekizuka T, Yamashita A, Kuroda M, Fujii Y et al. Distribution and relationships of antimicrobial resistance determinants among extended-spectrum-cephalosporin-resistant or carbapenem-resistant Escherichia coli isolates from rivers and sewage treatment plants in India. Antimicrob Agents Chemother 2016;60:2972–2980 [CrossRef]
    [Google Scholar]
  18. Beg AZ, Khan AU. Genome analyses of blaNDM-4 carrying ST 315 Escherichia coli isolate from sewage water of one of the Indian hospitals. Gut Pathog 2018;10:17 [CrossRef]
    [Google Scholar]
  19. Skariyachan S, Lokesh P, Rao R, Kumar AU, Vasist KS et al. A pilot study on water pollution and characterization of multidrug-resistant superbugs from Byramangala tank, Ramanagara district, Karnataka, India. Environ Monit Assess 2013;185:5483–5495 [CrossRef]
    [Google Scholar]
  20. Yao Y, Lazaro-Perona F, Falgenhauer L, Valverde A, Imirzalioglu C et al. Insights into a novel blaKPC-2-encoding IncP-6 plasmid reveal carbapenem-resistance circulation in several Enterobacteriaceae species from wastewater and a hospital source in Spain. Front Microbiol 2017;8:1143 [CrossRef]
    [Google Scholar]
  21. Apisarnthanarak A, Kiratisin P, Mundy LM. Evaluation of Ochrobactrum intermedium bacteremia in a patient with bladder cancer. Diagn Microbiol Infect Dis 2005;53:153–155 [CrossRef]
    [Google Scholar]
  22. Vaidya SA, Citron DM, Fine MB, Murakami G, Goldstein EJC. Pelvic abscess due to Ochrobactrum intermedium [corrected] in an immunocompetent host: case report and review of the literature. J Clin Microbiol 2006;44:1184–1186 [CrossRef]
    [Google Scholar]
  23. Zurfluh K, Bagutti C, Brodmann P, Alt M, Schulze J et al. Wastewater is a reservoir for clinically relevant carbapenemase- and 16S rRNA methylase-producing Enterobacteriaceae. Int J Antimicrob Agents 2017;50:436–440 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/acmi/10.1099/acmi.0.000024
Loading
/content/journal/acmi/10.1099/acmi.0.000024
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error