Table 2

CRESS-DNA viral genomes identified in dragonflies DfasCV, dragonfly-associated circular virus; DfasM, dragonfly-associated microphage; DfCirV, dragonfly circularisvirus; DfCyclV, dragonfly cyclicusvirus; DfOrv, dragonfly orbiculatusvirus.

Dragonfly species Detection method* Genome ID† GenBank accession no. Top match (Rep pairwise aa identity, %) Genus‡
(Say 1840) RE digestion (RV) DfCyV-3 (FL2-5E-2010) JX185424 Human faeces cyclovirus PK5034 (48 %) Cyclovirus
(Nielsen 1935) PCR DfCyV-4 (BG-NZ46-2007) JX185425 Bat faeces cycloviruses (90 %) Cyclovirus
(Fabricius 1798) RE digestion (RV) DfCirV (TO-DF3E-2010) JX185415 Marine circo-like virus CB-A (36.9 %) Novel
(Fabricius 1798) PCR DfCyclV (FL1-NZ37-2010) JX185418 Bat faeces TM6c circovirus (43.8 %) Novel
(Selys in Sagra 1857) RE digestion (I) DfasCV-1 (FL1-2X-2010) JX185429 SsHADV-1 like virus from badger faeces (51 %) Proposed Gemycircularvirus
(Say 1840) RE digestion (I) DfasCV-2 (FL2-5X-2010) JX185430 SsHADV-1 like virus from cassava (72 %) Proposed Gemycircularvirus
(Fabricius 1798) RE digestion (HI) DfasCV-3 (TO-DFS3B2-2010) JX185428 Mycovirus SsHADV-1 (44 %) Proposed Gemycircularvirus
(Hagen 1861) RE digestion (I) DfasM-1 (FL1-NZ54-2010) JX185431 phage 4 (29.7 %) Novel

*Refers to the method used to discover a given virus, including: RCA followed by RE digestion (specific enzyme is listed within parentheses) or degenerate circovirus PCR.

The origin of the sample (i.e. TO, Kingdom of Tonga; FL, Florida, USA, sites 1–3; BG, Bulgaria; PR, Puerto Rico), laboratory identification name and year of collection is given within parentheses. Genomes that share >95 % nt genome-wide identity have the same name and are in bold.

Several of the viruses detected during this study cannot be classified within known CRESS-DNA viral genera.