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Volume 162, Issue 10

An lung model to study bronchioles infected with biofilms Free

Abstract

A key aim in microbiology is to determine the genetic and phenotypic bases of bacterial virulence, persistence and antimicrobial resistance in chronic biofilm infections. This requires tractable, high-throughput models that reflect the physical and chemical environment encountered in specific infection contexts. Such models will increase the predictive power of microbiological experiments and provide platforms for enhanced testing of novel antibacterial or antivirulence therapies. We present an optimized model of cystic fibrosis lung infection: culture of pig bronchiolar tissue in artificial cystic fibrosis mucus. We focus on the formation of biofilms by . We show highly repeatable and specific formation of biofilms that resemble clinical biofilms by a commonly studied laboratory strain and ten cystic fibrosis isolates of this key opportunistic pathogen.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 3Rs , biofilm , chronic infection , cystic fibrosis , Pseudomonas aeruginosa and respiratory infection
© 2016 The Authors
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2016-10-01
2024-04-24
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.000352
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An ex vivo lung model to study bronchioles infected with Pseudomonas aeruginosa biofilms
Microbiology 162, 1755 (2016); https://doi.org/10.1099/mic.0.000352
/content/journal/micro/10.1099/mic.0.000352
/content/journal/micro/10.1099/mic.0.000352
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