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Volume 148, Issue 8

A zinc metalloprotease inhibitor, Inh, from the insect pathogen Free

Abstract

The entomopathogen secretes many proteins during the late stages of insect larvae infection and during laboratory culture. The authors have previously characterized and purified a 55 kDa zinc metalloprotease, PrtA, from culture supernatants of . PrtA is secreted via a classical type I secretory pathway and is encoded within the operon . The 405 bp gene encodes a 148 kDa pre-protein that is translocated to the periplasm by the classical signal-peptide-dependent pathway, yielding the mature 119 kDa inhibitor Inh. Inh is a specific inhibitor of the protease PrtA. This study describes the purification of Inh and the initial characterization of its protease inhibition properties.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): APR, alkaline protease , APRin, alkaline protease inhibitor , entomopathogen , Inh, inhibitor protein , protease inhibitor and protein purification
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2002-08-01
2024-04-24
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A zinc metalloprotease inhibitor, Inh, from the insect pathogen Photorhabdus luminescens
Microbiology 148, 2427 (2002); https://doi.org/10.1099/00221287-148-8-2427
/content/journal/micro/10.1099/00221287-148-8-2427
/content/journal/micro/10.1099/00221287-148-8-2427
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