Improvement of bacterial β-glucanase thermostability by glycosylation

References

1. Borriss R., Olsen O., Thomsen K. K., von Wettstein D. 1989; Hybrid Bacillusendo-(1,3-1,4)-β-glucanases : construction of recombinant genes and molecular properties of the gene products. Carlsberg Research Communications 54:41–54
   [Google Scholar]
2. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry 72:248–254
   [Google Scholar]
3. Curry C., Gilkes N., O’Neill G., Miller R. C., Skipper N. 1988; Expression and secretion of a Cellumonas fimiexoglucanase in Saccharomyces cerevisiae . Applied and Environmental Microbiology 54:476–484
   [Google Scholar]
4. Flores-Carreon A., Hixson S. H., Gomez A., Shao M.-C., Krudy G. 1990; The processing of iV-linked glycans in yeast. Journal of Biological Chemistry 265:754–759
   [Google Scholar]
5. Golub E. I. 1988; One minute’ transformation of competent E. coliby plasmid DNA. Nucleic Acids Research 16:1641
   [Google Scholar]
6. Gu J., Matsuda T., Nakamura R., Ishiguro H., Ohkubo I., Sasaki M., Takahashi N. 1989; Chemical deglycosylation of hen ovomucoid : protective effect of carbohydrate moiety on tryptic hydrolysis and heat dénaturation. Journal of Biochemistry 106:66–70
   [Google Scholar]
7. Hattori M., Sakaki Y. 1986; Dideoxy sequencing method using denatured plasmid templates. Analytical Chemistry 152:232–238
   [Google Scholar]
8. Innis M. A. 1989; Glycosylation of heterologous proteins in Saccharomyces cerevisiae . Yeast Genetic Engineering233–246 Barr P. J., Brake A. J., Valenzuela P. New York: Putten worths Publishers;
   [Google Scholar]
9. Ito H., Fukuda H. Y., Murata K., Kimura A. 1983; Transformation of intact yeast cells treated with alkali cations. Journal of Bacteriology 153:163–168
   [Google Scholar]
10. Jackson E. A., Ballance G. M., Thomsen K. K. 1986; Construction of a yeast vector directing the synthesis and release of barley (1→3,l→4)-β-glucanase. Carlsberg Research Communications 51:445–458
    [Google Scholar]
11. Kamman M., Laufs J., Schell J., Gronenborn B. 1989; Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR). Nucleic Acids Research 17:5404
    [Google Scholar]
12. Maley F., Trimble R. B., Tarentino A. L., Plummer T. H. 1989; Characterization of glycoproteins and their associated oligosaccharides through the use of endoglycosidases. Analytical Biochemistry 180:195–204
    [Google Scholar]
13. McCleary B. V. 1988; Soluble, dye-labelled polysaccharides for the assay of endohydrolases. Methods in Enzymology 160:74–86
    [Google Scholar]
14. Mellor J., Dobson M. J., Roberts N. A., Tuite M. F., Emtage J. S., White S., Lowe P. A., Patel T., Kingsman A. J., Kingsman S. M. 1983; Efficient synthesis of enzymatically active calf chymosin in Saccharomyces cerevisiae . Gene 24:1–14
    [Google Scholar]
15. Melnick L. M., Turner B. G., Puma P., Price-Tillotson B., Salvato K. A., Dumais D. R., Moir D. T., Broeze R. J., Averinos G. C. 1990; Characterization of a nonglycosylated single chain urinary plasminogen activator secreted from yeast. Journal of Biological Chemistry 265:801–807
    [Google Scholar]
16. Miller G. L. 1959; Use of dinitrosalicylic acid reagent for determination of reducing sugars. Analytical Chemistry 31:426–428
    [Google Scholar]
17. Nosoh Y., Sekiguchi T. 1990; Protein engineering for thermosta-bility. Trends in Biotechnology 8:16–20
    [Google Scholar]
18. Olden K., Bernard B. A., Humphries M. J., Yeo T.-K., Yeo K.-T., White S. L., Newton S. A., Bauer H. C., Parent J. B. 1985; Function of glycoprotein glycans. Trends in Biochemical Sciences 10:78–82
    [Google Scholar]
19. Olsen O., Thomsen K. K. 1989; Processing and secretion of barley (1-3,1 -4)-β-glucanase in yeast. Carlsberg Research Communications 54:29–39
    [Google Scholar]
20. Parekh R. B., Tse A. G. D., Dwek R. A., Williams A. F., Rademacher T. W. 1987; Tissue-specific N-glycosylation, site-specific oligosaccharide patterns and lentil lectin recognition of rat Thy-1. EM BO Journal 6:1233–1244
    [Google Scholar]
21. Petersen J. G. L., Kielland-Brandt M. C., Holmberg S., Nilsson-Tillgren T. 1983; Mutational analysis of isoleucine-valine biosynthesis in Saccharomyces cerevisiae . Mapping of ilv2 and ilvS. Carlsberg Research Communications 48:21–34
    [Google Scholar]
22. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
23. White C. A., Kennedy J. F. 1989; Oligosaccharides. Carbohydrate Analysis: a Practical Approach37–54 Chaplin M. F., Kennedy J. F. Oxford: IRL Press;
    [Google Scholar]
24. Yuuki T., Tezuka H., Yabuuchi S. 1989; Purification and some properties of two enzymes from a β-glucanase hyperproducing strain, Bacillus subtilisHL-25. Agricultural and Biological Chemistry 53:2341–2346
    [Google Scholar]
25. Zhang H., Schroll R., Browse J., Sommerville C. 1988; Double stranded sequencing as a choice for DNA sequencing. Nucleic Acids Research 16:1220
    [Google Scholar]