1887

Abstract

The continuing emergence of the multidrug resistance phenotype in Gram-negative bacteria makes the development of rapid susceptibility tests mandatory. To achieve this goal, proprietary specific media for bacterial growth can be used but may have some adverse effects. In this study, we dissected the role of media on porin, efflux pump and β-lactamase expression. Depending on the medium used, we observed a change in piperacillin-tazobactam susceptibility for some isolates, such as increases in MIC values. No significant alteration in efflux activity or in β-lactamase production was detected after changing the incubation medium. The ratio of piperacillinase:nitrocefinase showed no specific alteration, indicating that the various media did not affect significantly the relative enzymic affinity for the substrates. In contrast, osmotic variation was able to modulate both porin expression and OmpC : OmpF balance, thus modulating the antibiotic uptake. This study suggests that porin expression may be impacted by a susceptibility testing medium, which may modify the antibiotic diffusion into the bacteria, thus affecting MIC results.

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2015-11-01
2024-03-28
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References

  1. Acar J. F., Moulin G. 2012; Antimicrobial resistance: a complex issue. Rev Sci Tech 31:23–31[PubMed]
    [Google Scholar]
  2. Babini G. S., Yuan M., Hall L. M., Livermore D. M. 2003; Variable susceptibility to piperacillin/tazobactam amongst Klebsiella spp. with extended-spectrum β-lactamases. J Antimicrob Chemother 51:605–612 [View Article][PubMed]
    [Google Scholar]
  3. Bhattacharya S. 2013; Early diagnosis of resistant pathogens: how can it improve antimicrobial treatment?. Virulence 4:172–184 [View Article][PubMed]
    [Google Scholar]
  4. Bornet C., Saint N., Fetnaci L., Dupont M., Davin-Régli A., Bollet C., Pagès J. M. 2004; Omp35, a new Enterobacter aerogenes porin involved in selective susceptibility to cephalosporins. Antimicrob Agents Chemother 48:2153–2158 [View Article][PubMed]
    [Google Scholar]
  5. Burchak O. N., Pihive E. L., Maigre L., Guinchard X., Bouhours P., Jolivalt C., Schneider D., Maurin M., Giglione C., other authors. 2011; Synthesis and evaluation of 1-(1H-indol-3-yl)ethanamine derivatives as new antibacterial agents. Bioorg Med Chem 19:3204–3215 [View Article][PubMed]
    [Google Scholar]
  6. Burgess R. R., Deutscher M. P. 2009; Guide to protein purification. Methods Enzymol 463:88–89
    [Google Scholar]
  7. CLSI 2013 Performance Standards for Antimicrobial Susceptibility Testing 23rd informational supplement. CLSI document M100-S23 Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  8. Cohen J. 2013; Confronting the threat of multidrug-resistant Gram-negative bacteria in critically ill patients. J Antimicrob Chemother 68:490–491 [View Article][PubMed]
    [Google Scholar]
  9. Davin-Regli A., Bolla J. M., James C. E., Lavigne J. P., Chevalier J., Garnotel E., Molitor A., Pagès J. M. 2008; Membrane permeability and regulation of drug “influx and efflux” in enterobacterial pathogens. Curr Drug Targets 9:750–759 [View Article][PubMed]
    [Google Scholar]
  10. De la Cruz M. A., Calva E. 2010; The complexities of porin genetic regulation. J Mol Microbiol Biotechnol 18:24–36 [View Article][PubMed]
    [Google Scholar]
  11. Delihas N. 2012; Regulating the regulator: MicF RNA controls expression of the global regulator Lrp. Mol Microbiol 84:401–404 [View Article][PubMed]
    [Google Scholar]
  12. Dupont M., E., Chollet R., Chevalier J., Pagès J. M. 2004; Enterobacter aerogenes OmpX, a cation-selective channel mar- and osmo-regulated. FEBS Lett 569:27–30 [View Article][PubMed]
    [Google Scholar]
  13. Dupont M., James C. E., Chevalier J., Pagès J. M. 2007; An early response to environmental stress involves regulation of OmpX and OmpF, two enterobacterial outer membrane pore-forming proteins. Antimicrob Agents Chemother 51:3190–3198 [View Article][PubMed]
    [Google Scholar]
  14. Fernández L., Hancock R. E. 2012; Adaptive and mutational resistance: role of porins and efflux pumps in drug resistance. Clin Microbiol Rev 25:661–681 [View Article][PubMed]
    [Google Scholar]
  15. Grundmann H., Klugman K. P., Walsh T., Ramon-Pardo P., Sigauque B., Khan W., Laxminarayan R., Heddini A., Stelling J. 2011; A framework for global surveillance of antibiotic resistance. Drug Resist Updat 14:79–87 [View Article][PubMed]
    [Google Scholar]
  16. Jenkins S. G., Schuetz A. N. 2012; Current concepts in laboratory testing to guide antimicrobial therapy. Mayo Clin Proc 87:290–308 [View Article][PubMed]
    [Google Scholar]
  17. Jorgensen J. H., Ferraro M. J. 2009; Antimicrobial susceptibility testing: a review of general principles and contemporary practices. Clin Infect Dis 49:1749–1755 [View Article][PubMed]
    [Google Scholar]
  18. Khan A. S., Dancer S. J., Humphreys H. 2012; Priorities in the prevention and control of multidrug-resistant Enterobacteriaceae in hospitals. J Hosp Infect 82:85–93 [View Article][PubMed]
    [Google Scholar]
  19. Laxminarayan R., Duse A., Wattal C., Zaidi A. K. M., Wertheim H. F. L., Sumpradit N., Vlieghe E., Hara G. L., Gould I. M., other authors. 2013; Antibiotic resistance-the need for global solutions. Lancet Infect Dis 13:1057–1098 [View Article][PubMed]
    [Google Scholar]
  20. Lee S. Y., Yu J. K., Lee S. Y., Shin S. H., Park H. I., Oh E. J., Park Y. J. 2008; Evaluation of the VITEK 2 advanced expert system for reporting piperacillin susceptibility in Klebsiella spp. Antimicrob Agents Chemother 52:2291–2292 [View Article][PubMed]
    [Google Scholar]
  21. Liu X., Ferenci T. 2001; An analysis of multifactorial influences on the transcriptional control of ompF and ompC porin expression under nutrient limitation. Microbiology 147:2981–2989[PubMed] [CrossRef]
    [Google Scholar]
  22. Livermore D. M., Struelens M., Amorim J., Baquero F., Bille J., Canton R., Henning S., Gatermann S., Marchese A., other authors. 2002; Multicentre evaluation of the VITEK 2 Advanced Expert System for interpretive reading of antimicrobial resistance tests. J Antimicrob Chemother 49:289–300 [View Article][PubMed]
    [Google Scholar]
  23. Martínez J. L., Rojo F. 2011; Metabolic regulation of antibiotic resistance. FEMS Microbiol Rev 35:768–789 [View Article][PubMed]
    [Google Scholar]
  24. Masi M., Pagès J. M. 2013; Structure, function and regulation of outer membrane proteins involved in drug transport in Enterobacteriaceae: the OmpF/C – TolC Case. Open Microbiol J 7:22–33 [View Article][PubMed]
    [Google Scholar]
  25. Miller J. 1972; Experiments in Molecular Genetics, 2nd edn. Cold Spring Harbor. Cold Spring Harbor Laboratory;
    [Google Scholar]
  26. Nikaido H., Pagès J. M. 2012; Broad-specificity efflux pumps and their role in multidrug resistance of Gram-negative bacteria. FEMS Microbiol Rev 36:340–363 [View Article][PubMed]
    [Google Scholar]
  27. O'Callaghan C. H., Morris A., Kirby S. M., Shingler A. H. 1972; Novel method for detection of β-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother 1:283–288 [View Article][PubMed]
    [Google Scholar]
  28. Orenga S., James A. L., Manafi M., Perry J. D., Pincus D. H. 2009; Enzymatic substrates in microbiology. J Microbiol Methods 79:139–155 [View Article][PubMed]
    [Google Scholar]
  29. Pagès J. M., James C. E., Winterhalter M. 2008; The porin and the permeating antibiotic: a selective diffusion barrier in Gram-negative bacteria. Nat Rev Microbiol 6:893–903 [View Article][PubMed]
    [Google Scholar]
  30. Pagès J. M., Lavigne J. P., Leflon-Guibout V., Marcon E., Bert F., Noussair L., Nicolas-Chanoine M. H. 2009; Efflux pump, the masked side of β-lactam resistance in Klebsiella pneumoniae clinical isolates. PLoS One 4:e4817 [View Article][PubMed]
    [Google Scholar]
  31. Pagès J. M., Amaral L., Fanning S. 2011; An original deal for new molecule: reversal of efflux pump activity, a rational strategy to combat Gram-negative resistant bacteria. Curr Med Chem 18:2969–2980 [View Article][PubMed]
    [Google Scholar]
  32. Pitout J. D., Le P., Church D. L., Gregson D. B., Laupland K. B. 2008; Antimicrobial susceptibility of well-characterised multiresistant CTX-M-producing Escherichia coli: failure of automated systems to detect resistance to piperacillin/tazobactam. Int J Antimicrob Agents 32:333–338 [View Article][PubMed]
    [Google Scholar]
  33. Schuldiner S. 2009; EmrE, a model for studying evolution and mechanism of ion-coupled transporters. Biochim Biophys Acta 1794:748–762 [View Article][PubMed]
    [Google Scholar]
  34. Theuretzbacher U. 2012; Accelerating resistance, inadequate antibacterial drug pipelines and international responses. Int J Antimicrob Agents 39:295–299 [View Article][PubMed]
    [Google Scholar]
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