1887

Journal of Medical Microbiology logo

Volume 51, Issue 12

Development of a PCR assay specific for Free

Abstract

is a gram-positive anaerobic coccus that is widely distributed in the normal human flora. The organism has also been implicated as a causative agent of several systemic infections, including endocarditis and infections of the genitourinary and gastrointestinal tracts. Its role in oral disease is less well defined, although it has been implicated in periodontal disease, gingivitis and root canal infections. Identification of in clinical samples is currently reliant upon traditional culture and biochemical methods. The aim of this study was to develop a novel PCR assay for the detection of and to attempt detection of this organism in oral samples. PCR primers specific for DNA were developed by alignment of bacterial 16S ribosomal RNA gene sequences and selection of sequences specific at their 3′ ends for . When used in a PCR assay, positivity for DNA was indicated by the amplification of a 943-bp product. The primers were shown to be specific for DNA, as no PCR products were obtained when genomic DNA from a wide range of other species and other oral bacteria were used as templates. The PCR assay was then applied to the detection of DNA in subgingival plaque samples from adult periodontitis patients and pus aspirates from subjects with acute dento-alveolar abscesses. All of 60 subgingival plaque samples from 16 patients were negative for DNA. None of the 43 pus samples analysed contained DNA. These results suggest that is not a major pathogen in adult periodontitis and dento-alveolar abscesses. The PCR assay is a more rapid, sensitive and specific alternative to culture-based methods for identification of in clinical samples.

  • Published Online:
© 2002 Society for General Microbiology
Loading

Article metrics loading...

/content/journal/jmm/10.1099/0022-1317-51-12-1097
2002-12-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/51/12/1097.html?itemId=/content/journal/jmm/10.1099/0022-1317-51-12-1097&mimeType=html&fmt=ahah

References

  1. Holdeman Moore LV, Johnson JL, Moore WEC. Genus Peptostreptococcus . In Sneath PHA, Mair NS, Sharpe ME, Holt JG. eds Bergey's manual of systematic bacteriology vol 2Baltimore Williams & Wilkins: 19861083–1092
     [Google Scholar]
  2. Murdoch DA. Gram-positive anaerobic cocci. Clin Microbiol Rev 1998; 11:81–120
     [Google Scholar]
  3. Brook I. Recovery of anaerobic bacteria from a post-traumatic nasal septal abscess.A report of two cases. Ann Otol Rhin Laryngol 1998; 107:959–960 [CrossRef]
     [Google Scholar]
  4. Brook I, Frazier EH. Aerobic and anaerobic microbiology of infected hemorrhoids. Am J Gastroenterol 1996; 91:333–335
     [Google Scholar]
  5. Murdoch DA, Mitchelmore IJ, Tabaqchali S. The clinical importance of gram-positive anaerobic cocci isolated at St Bartholomew's Hospital. London: in 1987 J Med Microbiol 1994; 41:36–44 [CrossRef]
     [Google Scholar]
  6. Brook I. Anaerobic bacteria in suppurative genitourinary infections. J Urol 1989; 141:889–893
     [Google Scholar]
  7. Brook I, Frazier EH. Aerobic and anaerobic bacteriology of wounds and cutaneous abscesses. Arch Surg 1990; 125:1445–1451 [CrossRef]
     [Google Scholar]
  8. Montejo M, Ruiz-Irastorza G, Aguirrebengoa K, Amutio E, Hernandez JL, Aguirre C. Prosthetic-valve endocarditis caused by Peptostreptococcus anaerobius . Clin Infect Dis 1995; 20:1431
     [Google Scholar]
  9. Moore LVH, Moore WEC, Cato EP. et al. Bacteriology of human gingivitis. J Dent Res 1987; 66:989–995 [CrossRef]
     [Google Scholar]
  10. Wade WG, Moran J, Morgan JR, Newcombe R, Addy M. The effects of antimicrobial acrylic strips on the subgingival microflora in chronic periodontitis. J Clin Periodontol 1992; 19:127–134 [CrossRef]
     [Google Scholar]
  11. Moore WEC, Holdeman LV, Smibert RM, Hash DE, Burmeister JA, Ranney RR. Bacteriology of severe periodontitis in young adult humans. Infect Immun 1982; 38:1137–1148
     [Google Scholar]
  12. Sundqvist G. Associations between microbial species in dental root canal infections. Oral Microbiol Immunol 1992; 7:257–262 [CrossRef]
     [Google Scholar]
  13. Civen R, Vaisanen M-L, Finegold SM. Peritonsillar abscess, retropharyngeal abscess, mediastinitis, and nonclostridial anaerobic myonecrosis: a case report. Clin Infect Dis 1993; 16 Suppl 4:S299–S303 [CrossRef]
     [Google Scholar]
  14. Marler LM, Siders JA, Wolters LC, Pettigrew Y, Skitt BL, Allen SD. Evaluation of the new RapID-ANA II system for the identification of clinical anaerobic isolates. J Clin Microbiol 1991; 29:874–878
     [Google Scholar]
  15. Murdoch DA, Mitchelmore IJ. The laboratory identification of gram-positive anaerobic cocci. J Med Microbiol 1991; 34:295–308 [CrossRef]
     [Google Scholar]
  16. Devereux J, Haeberli P, Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 1984; 12:387–395 [CrossRef]
     [Google Scholar]
  17. Riggio MP, Lennon A, Wray D. Detection of Helicobacter pylori DNA in recurrent aphthous stomatitis tissue by PCR. J Oral Pathol Med 2000; 29:507–513 [CrossRef]
     [Google Scholar]
  18. Ng J, Ng L-K, Chow AW, Dillon J-AR. Identification of five Peptostreptococcus species isolated predominantly from the female genital tract by using the Rapid ID32A system. J Clin Microbiol 1994; 32:1302–1307
     [Google Scholar]
  19. Wilson MJ, Hall V, Brazier J, Lewis MAO. Evaluation of a phenotypic scheme for the identification of ‘butyrate- producing’ Peptostreptococcus species. J Med Microbiol 2000; 49:747–751
     [Google Scholar]
  20. Riggio MP, Lennon A, Smith A. Detection of Peptostreptococcus micros DNA in clinical samples by PCR. J Med Microbiol 2000; 50:249–254
     [Google Scholar]
  21. Hall ER, Martin SA, Suzuki JB, Falkler WA. The gingival immune response to periodontal pathogens in juvenile periodontitis. Oral Microbiol Immunol 1994; 9:327–334 [CrossRef]
     [Google Scholar]
  22. Paster BJ, Russell JB, Yang CMJ, Chow JM, Woese CR, Tanner R. Phylogeny of the ammonia-producing ruminal bacteria Peptostreptococcus anerobius , Clostridium sticklandii , and Clostridium aminophilum sp. nov. Int J Syst Bacteriol 1993; 43:107–110 [CrossRef]
     [Google Scholar]
  23. Li N, Hashimoto Y, Ezaki T. Determination of 16S ribosomal RNA sequences of all members of the genus Peptostreptococccus and their phylogenetic position. FEMS Microbiol Lett 1994; 116:1–5 [CrossRef]
     [Google Scholar]
  24. Ezaki T, Li N, Hashimoto Y, Miura H, Yamamoto H. 16S ribosomal DNA sequences of anaerobic cocci and proposal of Ruminococcus hansenii comb. nov. and Ruminococcus productus comb. nov. Int J Syst Bacteriol 1994; 44:130–136 [CrossRef]
     [Google Scholar]
  25. Murdoch DA, Shah HN. Reclassification of Peptostreptococcus magnus (Prevot 1933) Holdeman and Moore 1972 as Finegoldia magna comb.nov. and Peptostreptococcus micros (Prevot 1933) Smith 1957 as Micromonas micros comb. nov. Anaerobe 1999; 5:555–559 [CrossRef]
     [Google Scholar]
  26. Murdoch DA, Shah HN, Gharbia SE, Rajendram D. Proposal to restrict the genus Peptostreptococcus (Kluyver & van Niel 1936) to Peptostreptococcus anaerobius . Anaerobe 2000; 6:257–260 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/0022-1317-51-12-1097
Loading
Development of a PCR assay specific for Peptostreptococcus anaerobius
J. Med. Microbiol. 51, 1097 (2002); https://doi.org/10.1099/0022-1317-51-12-1097
/content/journal/jmm/10.1099/0022-1317-51-12-1097
/content/journal/jmm/10.1099/0022-1317-51-12-1097
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error