1887

Abstract

SUMMARY

Spread of herpes simplex virus to and within the mouse eye after inoculation of the cornea or the skin of the snout was examined by peroxidase-antiperoxidase (PAP) staining of viral antigen in flat mounts of the eye and by isolation of virus from nervous tissue. Following inoculation of virus at either site, viral antigen was found in ocular nerves. One to three days later antigen was also found in the iris, ciliary body and choroid/sclera suggesting that virus spread to these tissues occurred via their nerve supply. Viral antigen was also found in the retina of the uninoculated eye after corneal inoculation. After inoculation of the snout, virus was isolated from ophthalmic and maxillary parts of the trigeminal ganglion and the superior cervical ganglion and then from the brainstem, eye and mandibular part of the trigeminal ganglion. This sequence also suggested that virus reached the eye via the nerves and that this may occur indirectly via the brainstem. The PAP method allows rapid determination of the distribution of antigen in various tissues. Our observations suggest that widespread involvement of ocular tissue may occur by spread of virus in nerves within the eye.

Keyword(s): HSV , immunoperoxidase and ocular nerves
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-12-2989
1987-12-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/12/JV0680122989.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-12-2989&mimeType=html&fmt=ahah

References

  1. Anderson J. R. 1984; An animal model of ocular herpes. Keratitis, retinitis and cataract in the mouse. British Journal of Experimental Pathology 65:283–297
    [Google Scholar]
  2. Arvidson B. 1979; Retrograde transport of horseradish peroxidase in sensory and adrenergic neurons following injection into the anterior chamber. Journal of Neurocytology 8:751–764
    [Google Scholar]
  3. Beatie J. C., Stilwell D. L. 1961; Innervation of the eye. Anatomical Record 141:45–61
    [Google Scholar]
  4. Blyth W. A., Harbour D. A., Hill T. J. 1984; Pathogenesis of zosteriform spread of herpes simplex virus in the mouse. Journal of General Virology 65:1477–1486
    [Google Scholar]
  5. Burstone M. S. 1957; Polyvinyl pyrrolidone as a mounting medium for stains for fat and azo-dye products. American Journal of Clinical Pathology 28:429–430
    [Google Scholar]
  6. Claoué C. M. P., Hodges T., Hill T. J., Blyth W. A., Easty D. L. 1987; The histology of the eye after zosteriform spread of herpes simplex virus in the mouse. British Journal of Experimental Pathology in press
    [Google Scholar]
  7. Finn P. J., Ferguson I. A., Renton F. I., Rush R. A. 1986; Nerve growth factor immunohistochemistry and biological activity in the rat iris. Journal of Neurocytology 15:169–176
    [Google Scholar]
  8. Gregg I. M., Dixon A. D. 1973; Somatotopic organisation of the trigeminal ganglion in the rat. Archives of Oral Biology 18:487–498
    [Google Scholar]
  9. Hill T. J. 1987; Ocular pathogenicity of herpes simplex virus. Current Eye Research 6:1–7
    [Google Scholar]
  10. Hill T. J., Field H. J., Blyth W. A. 1975; Acute and recurrent infection with herpes simplex virus in the mouse : a model for studying latency and recurrent disease. Journal of General Virology 28:341–353
    [Google Scholar]
  11. Johnson R. T. 1964; The pathogenesis of herpes virus encephalitis. Journal of Experimental Medicine 119:343–356
    [Google Scholar]
  12. Lycke E., Kristensson K., Svennerholm B., Vahlne A., Ziegler R. 1984; Uptake and transport of herpes simplex virus in neurites of rat dorsal root ganglia cells in culture. Journal of General Virology 65:55–64
    [Google Scholar]
  13. Palmgren A. 1948; A rapid method for selective silver staining of nerve fibres and nerve endings in mounted paraffin sections. Acta zoologica 29:377–392
    [Google Scholar]
  14. Peeler J., Neiderkorn J., Matoba A. 1985; Corneal allografts induce cytotoxic T cells but not delayed hypersensitivity in mice. Investigative Ophthalmology and Visual Science 26:1516–1523
    [Google Scholar]
  15. Shimeld C., Easty D. L., Tullo A. B., Blyth W. A., Hill T. J. 1985a; Spread of herpes simplex virus to the eye following cutaneous inoculation in the skin of the snout of the mouse. In Herpetic Eye Disease pp 39–47 Maudgal P. C., Missotten L. Edited by Dordrecht: Dr. W. Junk;
    [Google Scholar]
  16. Shimeld C., Tullo A. B., Hill T. J., Blyth W. A., Easty D. L. 1985b; Spread of herpes simplex virus and distribution of latent infection after intraocular infection of the mouse. Archives of Virology85–175
    [Google Scholar]
  17. Shimeld C., Lewkowicz-Moss S. J., Lipworth K. M., Hill T. J., Blyth W. A., Easty D. L. 1986; Antigens of herpes simplex virus in whole corneal epithelial sheets from mice. Archives of Ophthalmology 104:1830–1834
    [Google Scholar]
  18. Shimeld C., Dyson H., Lewkowicz-Moss S. J., Hill T. J., Blyth W. A., Easty D. L. 1987; Spread of HSV-1 to the mouse eye after inoculation of the skin of the snout requires an intact nerve supply to the inoculation site. Current Eye Research 6:9–12
    [Google Scholar]
  19. Tullo A. B., Shimeld C., Blyth W. A., Hill T. J., Easty D. L. 1982a; Spread of virus and distribution of latent infection following ocular herpes simplex in the non-immune and immune mouse. Journal of General Virology 63:95–101
    [Google Scholar]
  20. Tullo A. B., Easty D. L., Hill T. J., Blyth W. A. 1982b; Ocular herpes simplex and the establishment of latent infection. Transactions of the Ophthalmological Society of the United Kingdom 102:15–18
    [Google Scholar]
  21. Tullo A. B., Shimeld C., Blyth W. A., Hill T. J., Easty D. L. 1983; Ocular infection with herpes simplex virus in non immune and immune mice. Archives of Ophthalmology 101:961–964
    [Google Scholar]
  22. Whittum J. A., Mcculley J. P., Niederkorn J. Y., Streilein J. W. 1984; Ocular disease induced in mice by anterior chamber inoculation of herpes simplex virus. Investigative Ophthalmology and Visual Science 25:1065–1073
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-12-2989
Loading
/content/journal/jgv/10.1099/0022-1317-68-12-2989
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error