1887

Abstract

SUMMARY

A virus (MnPV) with the structural characteristics of papilloma viruses was isolated from benign and malignant proliferations of adult animals of the inbred line ‘GRA Giessen’ of . The particles can be banded in CsCl gradients at densities of 1·34 g/ml (full particles) and 1·29 g/ml (empty particles). The virus DNA has a buoyant density of 1·7104 g/ml and can exist in three different conformations (supercoiled circular, nicked circular and linear), the sedimentation values of which have been determined as 23 to 24S, 16 to 17S and 14 to 15S, respectively. Although the mol. wt. of MnPV DNA is similar to that of HPV 1 DNA, the size of the fragments obtained after cleavage of MnPV DNA with the restriction endonuclease III is quite different from the pattern seen with human papilloma virus. The virion contains 12 different polypeptides; the major structural protein has a mol. wt. of 56000. MnPV is shown to be the causative agent of the skin proliferations, because tumours can be induced by inoculation of purified virus, whereas no cutaneous alterations are observed when the particles are inoculated in the presence of anti-MnPV serum. MnPV can be re-isolated from the experimentally induced tumours.

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1978-11-01
2024-03-28
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References

  1. Burtscher H., Grunberg W., Meingassner G. 1973; Infektiöse Keratoakanthome by Praomys (Mastomys) natalensis. Naturwissenschaften 60:209–210
    [Google Scholar]
  2. Crawford L. V., Crawford E. M. 1963; A comparative study of polyoma and papilloma viruses. Virology 21:258–263
    [Google Scholar]
  3. Gissmann L. 1977; Vergleichende Charakterisierung der Desoxyribonucleinsäuren menschlicher Papillom-viren. Doctoral thesis University of Erlangen-Nürnberg;
    [Google Scholar]
  4. Gissmann L., Zur hausen H. 1976; Human papilloma viruses: physical mapping and genetic heterogeneity. Proceedings of the National Academy of Sciences of the United States of America 73:1310–1313
    [Google Scholar]
  5. Gissmann L., Pfister H., Zur hausen H. 1977; Human papilloma viruses (HPV): characterization of four different isolates. Virology 76:569–580
    [Google Scholar]
  6. Ifft J. B., Voet D. H., Vinograd J. 1961; The determination of density distributions and density gradients in binary solutions at equilibrium in the ultracentrifuge. Journal of Physical Chemistry 65:1138–1145
    [Google Scholar]
  7. Lancaster W. D., Olson C., Meinke W. 1976; Quantitation of bovine papilloma viral DNA in viral-induced tumors. Journal of Virology 17:824–831
    [Google Scholar]
  8. Maizel J. V. Jun 1971 In Methods in Virology 5179–246 Maramorosch K., and Koprowsky H. London and New York: Academic Press;
    [Google Scholar]
  9. Melnick J. D., Allison A. C., Butel J. S., Eckhart W., Eddy B. E., Kit S., Levine A. J., Miles J. A. R., Pagano J. S., Sachs L., Vonka V. 1974 Papovavihdae: Intervirology 3:106–120
    [Google Scholar]
  10. Porath J., Sundberg L. 1972; High capacity chemiesorbents for protein immobilization. Nature New Biology 238:261–262
    [Google Scholar]
  11. Porath J., Axen R., Ernback S. 1967; Chemical coupling of proteins to agarose. Nature, London 215:1491–1492
    [Google Scholar]
  12. Rudolph R., Müller H. 1976; Induktion von epidermalem Tumorwachstum in der Haut von Mastomys natalensis durch Übertragung virushaltigen Tumorgewebes eines Platten-Epithelkarzinoms. Zentralblatt für Veterinärmedizin B23:143–150
    [Google Scholar]
  13. Rudolph R., Thiel W. 1976; Pathologische Anatomie und Histologie von spontanen, epithelialen Hauttumoren bei. Mastomys natalensis. Zentralblatt für Veterinärmedizin A23:429–441
    [Google Scholar]
  14. Schildkraut C L., Marmur J., Doty P. 1962; Determination of the base composition of desoxyribonucleic acids from its buoyant density in CsCl. Journal of Molecular Biology 4:430–443
    [Google Scholar]
  15. Seehafer J. A., Salmi A., Scraba D. G., Colter J. S. 1975; A comparative study of BK and polyoma viruses. Virology 66:192–205
    [Google Scholar]
  16. Shapiro A. L., Vinuela E., Maizel J. V. Jun 1967; Molecular weight estimation of polypeptide chains electrophoresis in SDS-polyacrylamide gels. Biochemical and Biophysical Research Communications 28:815–820
    [Google Scholar]
  17. Tai H. R., Smith C A., Sharp P. A., Vinograd J. 1972; Sequence heterogeneity in closed simian virus 40 desoxyribonucleic acid. Journal of Virology 9:317–325
    [Google Scholar]
  18. Texdorf I. 1967; Mastomys natalensis (SMITH, 1843) und ihre Eignung als Endwirt für Trematoden im Vergleich zu anderen Laboratoriumstieren. Doctoral thesis University of Giessen;
    [Google Scholar]
  19. Watson J. D, Littlefield J. W. 1960; Some properties of DNA of Shope papilloma virus. Journal of Molecular Biology 2:161–172
    [Google Scholar]
  20. Weigle J. M., Meselson M., Paigen K. 1959; Density alteration associated with transducing ability in the phage Lambda. Journal of Molecular Biology 1:379–386
    [Google Scholar]
  21. Yang R. C. A., Van De Voorde A., Hers W. 1976; Cleavage map of the simian-virus-40 genome by the restriction endonuclease III of Haemophilus aegyptius. European Journal of Biochemistry 61:101–118
    [Google Scholar]
  22. Zur Hausen h. 1977; Human papilloma viruses and their possible role in squamous cell carcinomas. Current Topics in Microbiology and Immunology 78:1–30
    [Google Scholar]
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