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Volume 65, Issue Pt_8

Characterization of the microaerophilic, bacteriochlorophyll -containing bacterium sp. nov., and emended descriptions of the genus and Free

Abstract

A red-pigmented, bacteriochlorophyll (BChl) -producing strain, AP64, was isolated previously from the freshwater Swan Lake located in the western Gobi Desert. Based on its 16S rRNA gene sequence identity (96.1 %) to the type strain T-27, the new isolate was tentatively classified as a member of the bacterial phylum . Here, we report its formal description and polyphasic characterization. Strain AP64 grew best on agar media under 9.8–15.2 % atmospheric oxygen. The cells were rods, dividing by symmetrical or asymmetrical binary fission. Budding structures were also observed. Its genomic DNA G+C content was 64.4 % (from the draft genome sequence). Phylogenetic analysis based on the 16S rRNA gene sequence clearly separated AP64 from related species. Its genotypic differentiation from phylogenetically close relatives was further supported by performing DNA–DNA hybridization and calculating average nucleotide identity, whereas the high percentage (67.3 %) of shared conserved proteins between strain AP64 and T-27 supports the classification of the two strains into the same genus. Strain AP64 contained C, C and Cω9 as predominant fatty acids. The main respiratory quinone was menaquinone 8 (MK-8). The most distinctive feature of strain AP64 was the presence of fully functional purple bacterial photosynthetic reaction centres. The main CO-fixation pathways were absent. Strain AP64 was capable of growth and BChl production in constant darkness. Thus, strain AP64 is a facultatively photoheterotrophic organism. It represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is AP64 ( = DSM 29774 = MCCC 1K00454). Emended descriptions of the genus and are also provided.

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Erratum

An erratum has been published for this content:
Erratum: Characterization of the microaerophilic, bacteriochlorophyll -containing bacterium sp. nov., and emended descriptions of the genus and
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2015-08-01
2024-04-23
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Characterization of the microaerophilic, bacteriochlorophyll a-containing bacterium Gemmatimonas phototrophica sp. nov., and emended descriptions of the genus Gemmatimonas and Gemmatimonas aurantiaca
Int J Syst Evol Microbiol 65, 2410 (2015); https://doi.org/10.1099/ijs.0.000272
/content/journal/ijsem/10.1099/ijs.0.000272
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