1887

Abstract

A psychrotolerant, Gram-stain-negative, motile, aerobic, peritrichous bacterium, strain DJC1-1, was isolated from Lake Dajiaco, Tibetan Plateau, China. The strain was negative for citrate utilization, lipase activity and α-glucosidase, but positive for the Voges–Proskauer reaction and -acetyl-β-glucosaminidase. 16S rRNA gene sequence analysis indicated that ATCC 29927, ATCC 13337, DSM 30063 and DSM 4540 were the closest relatives of strain DJC1-1, with similarities of 97.76, 96.80, 97.71 and 97.58 %, respectively. The DNA G+C content of strain DJC1-1 was 53.9 mol%. The predominant fatty acids were C and C cyclo. Based on these characteristics, strain DJC1-1 can be assigned to the genus . In DNA–DNA hybridization tests, strain DJC1-1 shared 50.6, 35.1, 36.5 and 18.1 % DNA–DNA relatedness with the type strains of , , and , respectively. The growth temperature ranged from 0 to 40 °C, with optimum growth at 15 °C. Physiological and biochemical tests differentiated strain DJC1-1 from the type strains of recognized species of the genus . Therefore, strain DJC1-1 is identified as representing a novel species of the genus for which the name sp. nov. is proposed. The type strain is DJC1-1 ( = JCM 30077 = CGMCC1.12806).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31100369, 41201059, 41171050 and 41371084)
  • National Basic Research Program of China (Award 2012CB956100)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000049
2015-03-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/3/971.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000049&mimeType=html&fmt=ahah

References

  1. Brenner D. J. ( 1978 ). Characterization and clinical identification of Enterobacteriaceae by DNA hybridization. . Prog Clin Pathol 7, 71117.[PubMed]
    [Google Scholar]
  2. Breznak J. A., Costilow R. N. ( 1994 ). Physicochemical factors in growth. . In Methods for General and Molecular Bacteriology, pp. 137154. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [View Article] [PubMed]
    [Google Scholar]
  4. Embley T. M. ( 1991 ). The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes. . Lett Appl Microbiol 13, 171174. [View Article] [PubMed]
    [Google Scholar]
  5. Gevers D., Huys G., Swings J. ( 2001 ). Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. . FEMS Microbiol Lett 205, 3136. [View Article] [PubMed]
    [Google Scholar]
  6. Greipsson S., Priest F. G. ( 1983 ). Numerical taxonomy of Hafnia alvei . . Int J Syst Bacteriol 33, 470475. [View Article]
    [Google Scholar]
  7. Huss V. A., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184192. [View Article] [PubMed]
    [Google Scholar]
  8. Huys G., Cnockaert M., Abbott S. L., Janda J. M., Vandamme P. ( 2010 ). Hafnia paralvei sp. nov., formerly known as Hafnia alvei hybridization group 2. . Int J Syst Evol Microbiol 60, 17251728. [View Article] [PubMed]
    [Google Scholar]
  9. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  10. Kim M., Oh H.-S., Park S.-C., Chun J. ( 2014 ). Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. . Int J Syst Evol Microbiol 64, 346351. [View Article] [PubMed]
    [Google Scholar]
  11. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3, 208218. [View Article]
    [Google Scholar]
  12. Masco L., Huys G., Gevers D., Verbrugghen L., Swings J. ( 2003 ). Identification of Bifidobacterium species using rep-PCR fingerprinting. . Syst Appl Microbiol 26, 557563. [View Article] [PubMed]
    [Google Scholar]
  13. McBee M., Schauer D. ( 2006 ). The genus Hafnia . . In The Prokaryotes, pp. 215218. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. . New York:: Springer;. [View Article]
    [Google Scholar]
  14. Møller V. ( 1954 ). Distribution of amino acid decarboxylases in Enterobacteriaceae . . Acta Pathol Microbiol Scand 35, 259277. [View Article] [PubMed]
    [Google Scholar]
  15. Reasoner D. J., Geldreich E. E. ( 1985 ). A new medium for the enumeration and subculture of bacteria from potable water. . Appl Environ Microbiol 49, 17.[PubMed]
    [Google Scholar]
  16. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  17. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000049
Loading
/content/journal/ijsem/10.1099/ijs.0.000049
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error