1887

Abstract

A novel Gram-stain-negative, strictly aerobic, heterotrophic bacterium, designated 306, was isolated from near-surface (109 cm below the sea floor) sediments of the Gulf of Lions, in the Mediterranean Sea. Strain 306 grew at temperatures between 4 and 32 °C (optimum 17–22 °C), from pH 6.5 to 9.0 (optimum 8.0–9.0) and between 0.5 and 6.0 % (w/v) NaCl (optimum 2.0 %). Its DNA G+C content was 58.8 mol%. On the basis of 16S rRNA gene sequence similarity, the novel isolate belongs to the class and is related to the genus . It shares 98.7 % 16S rRNA sequence identity with its closest phylogenetic relative. It contained Q-10 as the only respiratory quinone, Cω7 and C as major fatty acids (>5 %) and phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, two unidentified lipids and an aminolipid as polar lipids. The chemotaxonomic data are consistent with the affiliation of strain 306 to the genus . Results of physiological experiments, biochemical tests and DNA–DNA hybridizations (with ) indicate that strain 306 is genetically and phenotypically distinct from the five species of the genus with validly published names. Strain 306 therefore represents a novel species, for which the name sp. nov. is proposed. The type strain is 306 ( = DSM 25627 = CIP 110369 = UBOCC 3187).

Funding
This study was supported by the:
  • Joint Research Unit UMR 6197 [Centre National de la Recherche Scientifique (CNRS)–Institut Français de Recherche pour l'Exploitation de la Mer (Ifremer)–Université de Bretagne Occidentale (UBO)]
  • Institut National des Sciences de l'Univers (INSU) (CNRS)
  • UBO
  • Région Bretagne
  • French Ministry of Higher Education and Research
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2013-09-01
2024-03-28
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