1887

Abstract

Three isolates of a slow-growing, non-chromogenic were grown from three sputum samples of a patient from the north-eastern Ceará state in Brazil. Identification at species level could not be obtained with PCR restriction analysis of the gene. In order to characterize the isolates we carried out phenotypic and genotypic tests. We sequenced the nearly complete 16S rRNA gene and obtained partial sequences of the (encoding the hypervariable region of the 65 kDa heat-shock protein) and (encoding the beta-subunit of RNA polymerase) genes. The three isolates turned out to be identical and most closely related to the species and . The results, however, showed significant differences between these species and the isolates studied, which led us to consider them members of a novel species for which we propose the name . The type strain is HF8705 ( = Fiocruz-INCQS/CMRVS P4051 = DSM 45731).

Funding
This study was supported by the:
  • Health Ministry of Brazil
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.046862-0
2013-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/7/2583.html?itemId=/content/journal/ijsem/10.1099/ijs.0.046862-0&mimeType=html&fmt=ahah

References

  1. Adékambi T., Colson P., Drancourt M. ( 2003 ). rpoB-based identification of nonpigmented and late-pigmenting rapidly growing mycobacteria. . J Clin Microbiol 41, 56995708. [View Article] [PubMed]
    [Google Scholar]
  2. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. ( 1997 ). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res 25, 33893402. [View Article] [PubMed]
    [Google Scholar]
  3. Bull T. J., Shanson D. C., Archard L. C., Yates M. D., Hamid M. E., Minnikin D. E. ( 1995 ). A new group (type 3) of Mycobacterium celatum isolated from AIDS patients in the London area. . Int J Syst Bacteriol 45, 861862. [View Article] [PubMed]
    [Google Scholar]
  4. Butler W. R., O’Connor S. P., Yakrus M. A., Smithwick R. W., Plikaytis B. B., Moss C. W., Floyd M. M., Woodley C. L., Kilburn J. O. & other authors ( 1993 ). Mycobacterium celatum sp. nov.. Int J Syst Bacteriol 43, 539548. [View Article] [PubMed]
    [Google Scholar]
  5. CDC ( 1996 ). Standardized method for HPLC identification of mycobacteria. Atlanta, GA:: Centers for Disease Prevention and Control, US Department of Health and Human Services, Public Health Service;.
    [Google Scholar]
  6. Devulder G., Pérouse de Montclos M., Flandrois J. P. ( 2005 ). A multigene approach to phylogenetic analysis using the genus Mycobacterium as a model. . Int J Syst Evol Microbiol 55, 293302. [View Article] [PubMed]
    [Google Scholar]
  7. Griffith D. E., Aksamit T., Brown-Elliott B. A., Catanzaro A., Daley C., Gordin F., Holland S. M., Horsburgh R., Huitt G. & other authors ( 2007 ). An official ATS/IDSA statement: diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases. . Am J Respir Crit Care Med 175, 367416. [View Article] [PubMed]
    [Google Scholar]
  8. Kent P. T., Kubica G. P. ( 1985 ). Public Health Mycobacteriology. A Guide for the Level III Laboratory. Atlanta, GA:: US Department of Health and Human Services;.
    [Google Scholar]
  9. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [View Article] [PubMed]
    [Google Scholar]
  10. Koukila-Kähkölä P., Springer B., Böttger E. C., Paulin L., Jantzen E., Katila M. L. ( 1995 ). Mycobacterium branderi sp. nov., a new potential human pathogen. . Int J Syst Bacteriol 45, 549553. [View Article] [PubMed]
    [Google Scholar]
  11. Okazaki M., Ohkusu K., Hata H., Ohnishi H., Sugahara K., Kawamura C., Fujiwara N., Matsumoto S., Nishiuchi Y. & other authors ( 2009 ). Mycobacterium kyorinense sp. nov., a novel, slow-growing species, related to Mycobacterium celatum, isolated from human clinical specimens. . Int J Syst Evol Microbiol 59, 13361341. [View Article] [PubMed]
    [Google Scholar]
  12. Stackebrandt E., Frederiksen W., Garrity G. M., Grimont P. A. D., Kämpfer P., Maiden M. C. J., Nesme X., Rosselló-Mora R., Swings J. & other authors ( 2002 ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. . Int J Syst Evol Microbiol 52, 10431047. [View Article] [PubMed]
    [Google Scholar]
  13. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  14. Telenti A., Marchesi F., Balz M., Bally F., Böttger E. C., Bodmer T. ( 1993 ). Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. . J Clin Microbiol 31, 175178. [PubMed]
    [Google Scholar]
  15. Tortoli E. ( 2006 ). The new mycobacteria: an update. . FEMS Immunol Med Microbiol 48, 159178. [View Article] [PubMed]
    [Google Scholar]
  16. Tortoli E. ( 2010 ). Standard operating procedure for optimal identification of mycobacteria using 16S rRNA gene sequences. . Stand Genomic Sci 3, 145152. [View Article] [PubMed]
    [Google Scholar]
  17. Tortoli E. ( 2012 ). Phylogeny of the genus Mycobacterium: many doubts, few certainties. . Infect Genet Evol 12, 827831. [View Article] [PubMed]
    [Google Scholar]
  18. Vincent V., Gutierres M. C. ( 2007 ). Mycobacterium: laboratory characteristics of slowly growing mycobacteria. . In Manual of Clinical Microbiology, , 9th edn., pp. 573588. Edited by Murray P. R., Baron E. J., Landry M. L., Jorgensen J. H., Pfaller M. A. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.046862-0
Loading
/content/journal/ijsem/10.1099/ijs.0.046862-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error