1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_5

gen. nov., sp. nov., isolated from the sea cucumber Free

Abstract

A novel strain, designated J221, was isolated from the intestine of a sea cucumber, , collected from earthen ponds in Qingdao, China. The strain was Gram-negative, oxidasepositive, aerobic, rod-shaped and motile by means of one to several polar flagella. Growth of strain J221 was observed at temperatures between 10 and 40 °C with optimum growth between 25 and 28 °C. The pH range for growth was 5.09.0 with optimum growth at pH 7.5–8.0. The dominant fatty acids were summed feature 3 (comprising Cω7 and/or Cω6 29.04 %), C (28.93 %) and Cω7 (26.15 %). The major polar lipids were phosphatidylglycerol and phosphatidylethanolamine. Diphosphatidylglycerol, an unknown aminolipid and an unknown aminophospholipid were present in moderate to minor amounts in the polar lipid profile. Strain J221 had Q-8 as the major respiratory quinone. The DNA G+C content of strain J221 was 46.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain J221 is a member of the It formed a distinct phyletic line with less than 91 % sequence similarity to any species within previously recognized genera. On the basis of this polyphasic taxonomic study, strain J221 should be classified as a representative of a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is J221 ( = CGMCC 1.10130 = NRRL B-51319).

  • Published Online:
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 40730847)
  • Shandong Provincial Programs for Science and Technology Development (Award 2011GSF12108)
  • China Postdoctoral Science Foundation (Award 2012M511493)
  • Special Fund for Shangdong Provincial Post-doctoral Innovative Programs (Award 201003058)
© 2013 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.043448-0
2013-05-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/5/1597.html?itemId=/content/journal/ijsem/10.1099/ijs.0.043448-0&mimeType=html&fmt=ahah

References

  1. Baumann L., Baumann P., Mandel M., Allen R. D. ( 1972 ). Taxonomy of aerobic marine eubacteria. . J Bacteriol 110, 402–429.[PubMed]
    [Google Scholar]
  2. Baumann P., Baumann L., Bowditch R. D., Beaman B. ( 1984 ). Taxonomy of Alteromonas: A. nigrifaciens sp. nov., nom. rev.; A. macleodii; and A. haloplanktis . . Int J Syst Bacteriol 34, 145–149. [View Article]
    [Google Scholar]
  3. Bowman J. P., Gosink J. J., McCammon S. A., Lewis T. E., Nichols D. S., Nichols P. D., Skerratt J. H., Staley J. T., McMeekin T. A. ( 1998 ). Colwellia demingiae sp. nov., Colwellia hornerae sp. nov., Colwellia rossensis sp. nov., and Colwellia psychrotropica sp. nov.: a psychrophilic Antarctic species with the ability to synthesize docosahexaenoic acid (22:ω63). . Int J Syst Bacteriol 48, 1171–1180. [View Article]
    [Google Scholar]
  4. Chen Y. G., Zhang Y. Q., Huang H. Y., Klenk H. P., Tang S. K., Huang K., Chen Q. H., Cui X. L., Li W. J. ( 2009a ). Halomonas zhanjiangensis sp. nov., a halophilic bacterium isolated from a sea urchin. . Int J Syst Evol Microbiol 59, 2888–2893. [View Article] [PubMed]
    [Google Scholar]
  5. Chen Y. G., Zhang Y. Q., Shi J. X., Xiao H. D., Tang S. K., Liu Z. X., Huang K., Cui X. L., Li W. J. ( 2009b ). Jeotgalicoccus marinus sp. nov., a marine bacterium isolated from a sea urchin. . Int J Syst Evol Microbiol 59, 1625–1629. [View Article] [PubMed]
    [Google Scholar]
  6. Chen Y. G., Zhang Y. Q., Xiao H. D., Liu Z. X., Yi L. B., Shi J. X., Zhi X. Y., Cui X. L., Li W. J. ( 2009 c). Pontibacillus halophilus sp. nov., a moderately halophilic bacterium isolated from a sea urchin. . Int J Syst Evol Microbiol 59, 1635–1639. [View Article] [PubMed]
    [Google Scholar]
  7. Chiu H.-H., Shieh W. Y., Lin S. Y., Tseng C.-M., Chiang P.-W., Wagner-Döbler I. ( 2007 ). Alteromonas tagae sp. nov. and Alteromonas simiduii sp. nov., mercury-resistant bacteria isolated from a Taiwanese estuary. . Int J Syst Evol Microbiol 57, 1209–1216. [View Article] [PubMed]
    [Google Scholar]
  8. Choi E. J., Kwon H. C., Koh H. Y., Kim Y. S., Yang H. O. ( 2010 ). Colwellia asteriadis sp. nov., a marine bacterium isolated from the starfish Asterias amurensis . . Int J Syst Evol Microbiol 60, 1952–1957. [View Article] [PubMed]
    [Google Scholar]
  9. Deming J. W., Somers L. K., Straube W. L., Swartz D. G., MacDonnel M. T. ( 1988 ). Isolation of an obligately barophilic bacterium and description of a new genus, Colwellia gen. nov.. Syst Appl Microbiol 10, 152–160. [View Article]
    [Google Scholar]
  10. Dong X.-Z., Cai M.-Y. (editors) ( 2001 ). Determination of biochemical properties. . In Manual for the Systematic Identification of General Bacteria, pp. 370–398. Beijing:: Science Press; (in Chinese).
     [Google Scholar]
  11. Gauthier G., Gauthier M., Christen R. ( 1995 ). Phylogenetic analysis of the genera Alteromonas, Shewanella, and Moritella using genes coding for small-subunit rRNA sequences and division of the genus Alteromonas into two genera, Alteromonas (emended) and Pseudoalteromonas gen. nov., and proposal of twelve new species combinations. . Int J Syst Bacteriol 45, 755–761. [View Article] [PubMed]
    [Google Scholar]
  12. Ivanova E. P., Mikhailov V. V. ( 2001 ). A new family, Alteromonadaceae fam. nov., including marine proteobacteria of the genera Alteromonas, Pseudoalteromonas, Idiomarina, and Colwellia . . Microbiology 70, 10–17. [View Article]
    [Google Scholar]
  13. Ivanova E. P., Romanenko L. A., Chun J., Matte M. H., Matte G. R., Mikhailov V. V., Svetashev V. I., Huq A., Maugel T., Colwell R. R. ( 2000 ). Idiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the Pacific Ocean, including descriptions of two species, Idiomarina abyssalis sp. nov. and Idiomarina zobellii sp. nov.. Int J Syst Evol Microbiol 50, 901–907. [View Article] [PubMed]
    [Google Scholar]
  14. Ivanova E. P., Gorshkova N. M., Sawabe T., Zhukova N. V., Hayashi K., Kurilenko V. V., Alexeeva Y., Buljan V., Nicolau D. V. & other authors ( 2004 ). Sulfitobacter delicatus sp. nov. and Sulfitobacter dubius sp. nov., respectively from a starfish (Stellaster equestris) and sea grass (Zostera marina). . Int J Syst Evol Microbiol 54, 475–480. [View Article] [PubMed]
    [Google Scholar]
  15. Ivanova E. P., Christen R., Gorshkova N. M., Zhukova N. V., Kurilenko V. V., Crawford R. J., Mikhailov V. V. ( 2010 ). Winogradskyella exilis sp. nov., isolated from the starfish Stellaster equestris, and emended description of the genus Winogradskyella . . Int J Syst Evol Microbiol 60, 1577–1580. [View Article] [PubMed]
    [Google Scholar]
  16. Jean W. D., Shieh W. Y., Liu T. Y. ( 2006 ). Thalassomonas agarivorans sp. nov., a marine agarolytic bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the genus Thalassomonas . . Int J Syst Evol Microbiol 56, 1245–1250. [View Article] [PubMed]
    [Google Scholar]
  17. Jordan E. M., Thompson F. L., Zhang X.-H., Li Y., Vancanneyt M., Kroppenstedt R. M., Priest F. G., Austin B. ( 2007 ). Sneathiella chinensis gen. nov., sp. nov., a novel marine alphaproteobacterium isolated from coastal sediment in Qingdao, China. . Int J Syst Evol Microbiol 57, 114–121. [View Article] [PubMed]
    [Google Scholar]
  18. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161–207. [View Article]
    [Google Scholar]
  19. Macián M. C., Ludwig W., Schleifer K. H., Garay E., Pujalte M. J. ( 2001 ). Thalassomonas viridans gen. nov., sp. nov., a novel marine γ-proteobacterium. . Int J Syst Evol Microbiol 51, 1283–1289.[PubMed] [CrossRef]
    [Google Scholar]
  20. Martínez-Checa F., Béjar V., Llamas I., Del Moral A., Quesada E. ( 2005 ). Alteromonas hispanica sp. nov., a polyunsaturated-fatty-acid-producing, halophilic bacterium isolated from Fuente de Piedra, southern Spain. . Int J Syst Evol Microbiol 55, 2385–2390. [View Article] [PubMed]
    [Google Scholar]
  21. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159–167. [View Article]
    [Google Scholar]
  22. Nedashkovskaya O. I., Vancanneyt M., Kim S. B. ( 2010 ). Bizionia echini sp. nov., isolated from a sea urchin. . Int J Syst Evol Microbiol 60, 928–931. [View Article] [PubMed]
    [Google Scholar]
  23. Neulinger S. C., Järnegren J., Ludvigsen M., Lochte K., Dullo W.-C. ( 2008 ). Phenotype-specific bacterial communities in the cold-water coral Lophelia pertusa (Scleractinia) and their implications for the coral’s nutrition, health, and distribution. . Appl Environ Microbiol 74, 7272–7285. [View Article] [PubMed]
    [Google Scholar]
  24. Romanenko L. A., Uchino M., Frolova G. M., Mikhailov V. V. ( 2007 ). Marixanthomonas ophiurae gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from a deep-sea brittle star. . Int J Syst Evol Microbiol 57, 457–462. [View Article] [PubMed]
    [Google Scholar]
  25. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  26. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  27. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125–128. [View Article]
    [Google Scholar]
  28. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 1596–1599. [View Article] [PubMed]
    [Google Scholar]
  29. Vandecandelaere I., Nercessian O., Segaert E., Achouak W., Mollica A., Faimali M., De Vos P., Vandamme P. ( 2008 ). Alteromonas genovensis sp. nov., isolated from a marine electroactive biofilm and emended description of Alteromonas macleodii Baumann et al. 1972 (approved lists 1980). . Int J Syst Evol Microbiol 58, 2589–2596. [View Article] [PubMed]
    [Google Scholar]
  30. Yi H., Bae K. S., Chun J. ( 2004 ). Aestuariibacter salexigens gen. nov., sp. nov. and Aestuariibacter halophilus sp. nov., isolated from tidal flat sediment, and emended description of Alteromonas macleodii . . Int J Syst Evol Microbiol 54, 571–576. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.043448-0
Loading
Neiella marina gen. nov., sp. nov., isolated from the sea cucumber Apostichopus japonicus
Int J Syst Evol Microbiol 63, 1597 (2013); https://doi.org/10.1099/ijs.0.043448-0
/content/journal/ijsem/10.1099/ijs.0.043448-0
/content/journal/ijsem/10.1099/ijs.0.043448-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error