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Volume 60, Issue 12

sp. nov., from the air of a duck barn Free

Abstract

A Gram-positive, non-spore-forming actinobacterium (Sj 10) was isolated on tryptone soy agar from the air of a duck barn after filter sampling. Based on 16S rRNA gene sequence similarity studies, strain Sj 10 was shown to belong to the genus and was closely related to subsp. L-1 (97.8 %), DSM 19811 (97.3 %) and RF6 (97.3 %). The peptidoglycan of strain Sj 10 contained 2,4-diaminobutyric acid in combination with a lower amount of lysine as diagnostic diamino acids. In addition, threonine, glycine, alanine and glutamic acid were found. Menaquinone MK-11 was the major respiratory quinone; MK-12 and MK-10 were detected in minor amounts. The polar lipid pattern consisted of phosphatidylglycerol, diphosphatidylglycerol and one unknown component each of a phospholipid, glycolipid and aminoglycolipid. Strain Sj 10 contained the major fatty acids anteiso-C, iso-C and anteiso-C, like other members of the genus . Results of DNA–DNA hybridization, physiological and biochemical tests enabled strain Sj 10 to be differentiated genotypically and phenotypically from the most closely related species. Strain Sj 10 represents a novel species of the genus , for which the name sp. nov. is proposed, with Sj 10 (=DSM 22806 =CCM 7705) as the type strain.

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Keyword(s): 2D-TLC, two-dimensional ascending TLC , DAB, diaminobutyric acid , pNA, para-nitroanilide and pNP, para-nitrophenyl
SGM
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2010-12-01
2024-04-23
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Leucobacter aerolatus sp. nov., from the air of a duck barn
Int J Syst Evol Microbiol 60, 2838 (2010); https://doi.org/10.1099/ijs.0.021303-0
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