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Volume 59, Issue 6

sp. nov., a thermophilic, chemolithoautotrophic, sulfur-reducing bacterium isolated from an East Pacific Rise hydrothermal vent Free

Abstract

A novel strictly anaerobic, thermophilic, sulfur-reducing bacterium, designated PH1209, was isolated from an East Pacific Rise hydrothermal vent (1 ° N) sample and studied using a polyphasic taxonomic approach. Cells were Gram-negative, motile rods (approx. 1.60×0.40 μm) with a single polar flagellum. Strain PH1209 grew at temperatures between 33 and 65 °C (optimum 60 °C), from pH 5.0 to 8.0 (optimum 6.0–6.5), and between 2 and 4 % (w/v) NaCl (optimum 3 %). Cells grew chemolithoautotrophically with H as an energy source, S as an electron acceptor and CO as a carbon source. Strain PH1209 was also able to use peptone and yeast extract as carbon sources. The G+C content of the genomic DNA was 35 mol%. Phylogenetic analyses based on 16S rRNA gene sequencing showed that strain PH1209 fell within the order , in the class . Comparative 16S rRNA gene sequence analysis indicated that strain PH1209 belonged to the genus and shared 97.2 and 98.7 % 16S rRNA gene sequence identity, respectively, with the type strains of and . It is proposed, from the polyphasic evidence, that the strain represents a novel species, sp. nov.; the type strain is PH1209 (=DSM 21157=JCM 15390).

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2009-06-01
2024-04-18
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Nautilia abyssi sp. nov., a thermophilic, chemolithoautotrophic, sulfur-reducing bacterium isolated from an East Pacific Rise hydrothermal vent
Int J Syst Evol Microbiol 59, 1310 (2009); https://doi.org/10.1099/ijs.0.005454-0
/content/journal/ijsem/10.1099/ijs.0.005454-0
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vol. , part 6, pp. 1310 - 1315

Scanning electron micrographs of cells of strain PH1209 in the mid-exponential phase of growth.

Maximum growth rate (h ) of Nautilia abyssi sp. nov. PH1209 at varying temperatures, pH and NaCl concentrations.

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