1887

Abstract

A free-living diazotrophic strain, DS2, was isolated from corn rhizosphere. Polyphasic taxonomy was performed including morphological characterization, Biolog analysis, and 16S rRNA, and gene sequence analyses. 16S rRNA gene sequence analysis indicated that strain DS2 was closely related to the genus (96 % similarity). Chemotaxonomic characteristics (DNA G+C content 67.9 mol%; Q-10 quinone system; major fatty acid 18 : 17) were also similar to those of the genus . In all the analyses, including phenotypic characterization using Biolog analysis and comparison of cellular fatty acids, this isolate was found to be different from the closely related species , and . On the basis of these results, a novel species is proposed for this nitrogen-fixing strain. The name sp. nov. is suggested with the type strain DS2 (=NCCB 100108=LMG 23617).

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2007-03-01
2024-03-28
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