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Volume 47, Issue 1

sp. nov., a New Species Found in Goats Free

Abstract

Six -like strains that formed a phenotypically homogeneous group were isolated from the nasal flora of healthy goats. Total genomic DNA-DNA hybridization, rRNA gene restriction pattern, DNA base composition, and genetic transformation studies were performed to determine the relationships of these bacteria to species belonging to the genus and other fastidious gram-negative species. The new group of isolates was very homogeneous, as shown by rRNA gene restriction fragment length patterns (ribotyping), and these organisms displayed high relative binding ratios (RBRs) to each other in DNA-DNA hybridization experiments (RBRs, = 58%) but distinctly lower levels of DNA homology with all other species investigated. However, the RBRs obtained with species of the genus were higher than the RBRs obtained with all other gram-negative strains examined. Although the new strains had most of the phenotypic characteristics except nitrate reduction, quantitative and qualitative genetic transformation data led to the conclusion that they belong to a distinct new cluster in the genus The results of this study, combined with the general morphological and phenotypic profiles of the new strains, are consistent with the creation of a new species, for which the name is proposed. Strain 88365 (= ATCC 700022 = CCUG 35435 = NCTC 12925 = CIP 104716) is the type strain of .

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Copyright © 1997 International Union of Microbiological Societies
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1997-01-01
2024-04-23
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References

  1. Andersen B. M., Steigerwalt A. G., O’Connor S. P., Hollis D. G., Weyant R. S., Weaver R. E., Brenner D. J. 1993; Neisseria weaveri sp. nov., formerly CDC group M-5, a gram-negative bacterium associated with dog bite wounds. J. Clin. Microbiol. 31:2456–2466
     [Google Scholar]
  2. Barber D. M. L. 1985; Bacterial population of slaughter cattle. Vet. Rec. 115:169–170
     [Google Scholar]
  3. Barber D. M. L., Jones G. E. 1988; An attempt to induce clinical infectious keratoconjunctivitis in calves with naturally occurring Moraxella bovis infection. Vet. Rec. 122:210–211
     [Google Scholar]
  4. Bøvre K. 1965; Studies on transformation in Moraxella and organisms assumed to be related to Moraxella. 3. Quantitative streptomycin resistance transformation between Moraxella bovis and Moraxella nonliquefaciens strains. Acta Pathol. Microbiol. Scand. 63:42–50
     [Google Scholar]
  5. Bøvre K. 1965; Studies on transformation in Moraxella and organisms assumed to be related to Moraxella. 5. Streptomycin resistance transformation between serum-liquefying, nonhaemolytic moraxellae, Moraxella bovis and Moraxella nonliquefaciens. Acta Pathol. Microbiol. Scand. 65:435–449
     [Google Scholar]
  6. Bøvre K. 1984; Genus II. Moraxella Lwoff 1939, 173 emend. Henriksen and Bøvre 1968, 391AL. 296–303 Krieg N. R., Holt J. B. N. R. Krieg and J. B. HoltBergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore, Md.:
     [Google Scholar]
  7. Bøvre K., Hägen N. 1980; The family Neisseriaceae: rod-shaped species of the genera Moraxella, Acinetobacter, Kingella, and Neisseria, and the Branhamella group of cocci. 1506–1529 Starr M. P. The prokaryotes. A handbook on habitats, isolation, and identification of bacteria II Springer-Verlag; Berlin, Germany:
     [Google Scholar]
  8. Cabassi E., Cattabiani F., Brindani F., Freschi E. 1975; La flora bactterica della mucosa nasale del cavallo. Nota I. Batteri Gram-negativi. Folia Vet. Lat. 5:55–85
     [Google Scholar]
  9. Catlin B. W. 1991; Branhamaceae fam. nov., a new proposed family to accommodate the genera Branhamella and Moraxella. Int. J. Syst. Bacteriol. 41:320–323
     [Google Scholar]
  10. Gerhardt R. R., Allen J. W., Greene W. H., Smith P. C. 1983; The role of face flies in an episode of infectious bovine conjunctivitis. J. Am. Vet. Med. Assoc. 180:156–159
     [Google Scholar]
  11. Henriksen S. D., Bøvre K. 1968; The taxonomy of the genera Moraxella and Neisseria. J. Gen. Microbiol. 51:387–392
     [Google Scholar]
  12. Hoke C., Vedros N. A. 1982; Taxonomy of the Neisseriaceae: deoxyribonucleic acid base composition, interspecific transformation, and deoxyribonucleic acid hybridization. Int. J. Syst. Bacteriol. 32:57–66
     [Google Scholar]
  13. Hoquet F., Higgins R., Lessard P., Vrins A., Marcoux M. 1985; Comparison of bacterial and fungal flora in the pharynx of normal horses and horses affected with pharyngitis. Can. Vet. J. 26:342–346
     [Google Scholar]
  14. Hughes D. E., Pugh G. W. 1970; Isolation and description of a Moraxella from horses with conjunctivitis. Am. J. Vet. Res. 31:457–459
     [Google Scholar]
  15. Huntington P. J., Coloe P. J., Bryden J. D., MacDonald F. 1987; Isolation of a Moraxella sp. from horse with conjunctivitis. Aust. Vet. J. 64:118–119
     [Google Scholar]
  16. Jannes G., Vaneechoutte M., Lannoo M., Gillis M., Vancanneyt M., Vandamme P., Verschraegen G., Van Heuverswyn H., Rossau R. 1993; Polyphasic taxonomy leading to the proposal of Moraxella canis sp. nov. for Moraxella catarrhalis-like strains. Int. J. Syst. Bacteriol. 43:438–449
     [Google Scholar]
  17. Juni E. 1974; Simple genetic transformation assay for rapid diagnosis of Moraxella osloensis. Appl. Microbiol. 27:16–24
     [Google Scholar]
  18. Juni E., Heym G. A., Maurer M. J., Miller M. L. 1987; Combined genetic transformation and nutritional assay for identification of Moraxella nonliquefaciens. J. Clin. Microbiol. 25:1691–1694
     [Google Scholar]
  19. Juni E., Heym G. A., Newcomb R. D. 1988; Identification of Moraxella bovis by qualitative genetic transformation and nutritional assays. Appl. Environ. Microbiol. 54:1304–1306
     [Google Scholar]
  20. Kodjo A., Dorier A., Lerondelle C., Richard Y. 1994; Isolation of a new biovar of Moraxella bovis from healthy caprine nasal swabs. Small Ruminant Res. 15:87–95
     [Google Scholar]
  21. Kodjo A., Exbrayat P., Richard Y. 1994; Identification of Moraxella bovis and related species from calves with IBK and goats by qualitative genetic transformation assay. J. Vet. Med. Ser. B 41:336–343
     [Google Scholar]
  22. Kodjo A., Moussa A., Borges E., Richard Y. 1993; Identification of Moraxella-like bacteria isolated from caprine and ovine nasal flora. J. Vet. Med. Ser. B 40:97–104
     [Google Scholar]
  23. Kodjo A., Tønjum T., Richard Y., Bøvre K. 1995; Moraxella caprae sp. nov., a new member of the classical moraxellae. Int. J. Syst. Bacteriol. 45:467–471
     [Google Scholar]
  24. Marmur J. G. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol. 3:208–218
     [Google Scholar]
  25. Pande P., Sekariah P. C. 1960; A preliminary note on the isolation of Moraxella caprae nov. sp. from an outbreak of infectious keratoconjunctivitis in goats. Curr. Sci. 29:276–277
     [Google Scholar]
  26. Peyret M., Freney J., Meugnier H., Fleurette J. 1989; Determination of G + C content using high-performance liquid chromatography for the identification of staphylococci and micrococci. Res. Microbiol. 140:467–475
     [Google Scholar]
  27. Ringvold A., Vik E., Bevanger L. S. 1985; Moraxella lacunata isolated from epidemic conjunctivitis among teenaged females. Acta Ophthalmol. 63:427–431
     [Google Scholar]
  28. Rossau R., Vanlandschoot A., Gillis M., De Ley J. 1991; Taxonomy of Moraxellaceae fam. nov., a new bacterial family to accommodate the genera Moraxella, Acinetobacter, and Psychrobacter and related organisms. Int. J. Syst. Bacteriol. 41:310–319
     [Google Scholar]
  29. Tønjum T., Bukholm G., Bøvre K. 1989; Differentiation of some species of Neisseriaceae and other bacterial groups by DNA-DNA hybridization. Acta Pathol. Microbiol. Immunol. Scand. 97:395–405
     [Google Scholar]
  30. Tønjum T., Bukholm G., Bøvre K. 1990; Identification of Haemophilus aphrophilus and Actinobacillus actinomycetemcomitans by DNA-DNA hybridization and genetic transformation. J. Clin. Microbiol. 28:1994–1998
     [Google Scholar]
  31. Tønjum T., Caugant D. A., Bøvre K. 1992; Differentiation of Moraxella nonliquefaciens, M. lacunata, and M. bovis by using multilocus enzyme electrophoresis and hybridization with pilin-specific DNA probes. J. Clin. Microbiol. 30:3099–3107
     [Google Scholar]
  32. Tønjum T., Hägen N., Bøvre K. 1985; Identification of Eikenella corrodens and Cardiobacterium hominis by genetic transformation. Acta Pathol. Microbiol. Immunol. Scand. Sect. B 93:386–394
     [Google Scholar]
  33. Travicek M., Dravecky T., Balascak J. 1982; Isolation of Chlamydia psittaci and Moraxella bovis from infectious keratoconjunctivitis in lambs. Vet. Med. (Prague) 27:491–496
     [Google Scholar]
  34. Vandamme P., Gillis M., Vancanneyt M., Hoste B., Kersters K., Falsen E. 1993; Moraxella lincolnii sp. nov. isolated from the human respiratory tract, and reevaluation of the taxonomic position of Moraxella osloensis. Int. J. Syst. Bacteriol. 43:474–481
     [Google Scholar]
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Moraxella boevrei sp. nov., a New Moraxella Species Found in Goats
Int J Syst Evol Microbiol 47, 115 (1997); https://doi.org/10.1099/00207713-47-1-115
/content/journal/ijsem/10.1099/00207713-47-1-115
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