1887

Abstract

Abstract

A recent epiphytotic disease on citrus in Florida nurseries was caused by strains of with different host specificity and lower pathogenic capacities than those of previously described strains of pv. The new strains were classified as pv. because they were isolated from rutaceous hosts and despite the fact that they caused a different disease than strains previously described in that pathovar. Restriction fragment length polymorphism analyses revealed that the Florida strains comprised a heterogeneous (E) group, interrelated with pv. pv. and pv. In contrast, the previously described strains of pv. formed two highly distinct, homogeneous (A and B/C/D) groups. Furthermore, the strains of pv. pv. pv. pv. pv. and pv. tested were also distinctive and appeared to be only distantly related to one another and to all pv. strains. We concluded (i) that some pathovars are sufficiently distinct from the type strain of the species to be considered as separate species, (ii) that pv. group A and pv. (not including pv. var. represent distinctly separate subbranches of the genus and should be respectively reinstated to species as (ex Hasse) nom. rev. 3213 and (ex Smith) nom. rev. G27, and (iii) that the pv. B/C/D strains and the heterogeneous E strains should be respectively renamed pv. pv. nov. and pv. pv. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-39-1-14
1989-01-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/39/1/ijs-39-1-14.html?itemId=/content/journal/ijsem/10.1099/00207713-39-1-14&mimeType=html&fmt=ahah

References

  1. Bradbury J. F. 1984; Xanthomonas Dowson 1939. 199–210 In Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1: The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  2. Cafati C. R., Saettler A. W. 1980; Effect of host on multiplication and distribution of bean common blight bacteria. Phytopathology 70:675–679
    [Google Scholar]
  3. Civerolo E. L. 1984; Bacterial canker disease of citrus. J. Rio Grande Val. Hortic. Soc. 37:127–146
    [Google Scholar]
  4. Claflin L. E., Sasser M. D., Vidaver A. K. 1985; MXP, a semi-selective medium for Xanthomonas campestris pv. phaseoli. Phytopathology 751360
    [Google Scholar]
  5. Gabriel D. W., Hunter J., Kingsley M., Miller J., Lazo G. 1988; Clonal population structure of Xanthomonas campestris and genetic diversity among citrus canker strains. Mol. Plant-Microbe Interact. 1:59–65
    [Google Scholar]
  6. Gerhardt P., Murray R. E. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. 1981 Manual of methods for general bacteriology American Society for Microbiology; Washington, D.C.:
    [Google Scholar]
  7. Goto M., Takahashi T., Messina M. A. 1980; A comparative study of the strains of Xanthomonas campestris pv. citri isolated from citrus canker in Japan and cancrosis B in Argentina. Phytopathol. Soc. Jpn. 46:329–338
    [Google Scholar]
  8. Gottwald T. R., Civerolo E. L., Garnsey S. M., Brlansky R. H., Graham J. H., Gabriel D. W. 1988; Dynamics and spatial distribution of Xanthomonas campestris pv. citri group E strains in simulated nursery and new grove situations. Plant Dis. 72:781–787
    [Google Scholar]
  9. Hartung J. S., Civerolo E. L. 1987; Genomic fingerprints of Xanthomonas campestris pv. citri strains from Asia, South America and Florida. Phytopathology 77:282–285
    [Google Scholar]
  10. Hildebrand D. C. 1971; Pectate and pectin gels for differentiation of Pseudomonas sp. and other bacterial plant pathogens. Phytopathology 62:1430–1436
    [Google Scholar]
  11. Johnson J. L. 1984; Nucleic acids in bacterial classification. 8–11 In Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1: The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  12. Klement Z. 1982; Hypersensitivity. 149–177 In Mount M. S., Lacy G. H. Phytopathogenic prokaryotes II: Academic Press, Inc.; New York:
    [Google Scholar]
  13. Lapage S. P., Sneath P. H. A., Lessei E. F., Skerman V. B. D., Seeliger H. P. R., Clark W. A. 1975 International code of nomenclature of bacteria American Society for Microbiology; Washington, D.C.:
    [Google Scholar]
  14. Lazo G. R., Gabriel D. W. 1987; Conservation of plasmid DNA sequences and pathovar identification of strains of Xanthomonas campestris. Phytopathology 77:448–453
    [Google Scholar]
  15. Lazo G. R., Gabriel D. W., Roffey R. 1986; Differentiating pathovars of Xanthomonas campestris without pathogenicity tests. Phytopathology 761076
    [Google Scholar]
  16. Lazo G. R., Roffey R., Gabriel D. W. 1987; Pathovars of Xanthomonas campestris are distinguishable by restriction fragment length polymorphisms. Int. J. Syst. Bacteriol. 37:214–221
    [Google Scholar]
  17. Lennette E. H., Spaulding E. H., Truant J. P. 1974; Manual of clinical microbiology. , 2nd. American Society for Microbiology Washington, D.C.:
    [Google Scholar]
  18. Neidhardt I. C., Bloch P. L., Smith D. F. 1974; Culture medium for enterobacteria. J. Bacteriol. 119:736–747
    [Google Scholar]
  19. Sands D. C., Hankin L., Zucker M. 1972; A selective medium for pectolytic fluorescent pseudomonads. Phytopathology 62:998–1000
    [Google Scholar]
  20. Schleifer K. H., Stackebrandt E. 1983; Molecular systematics of prokaryotes. Annu. Rev. Microbiol. 37:143–187
    [Google Scholar]
  21. Schoulties C. L., Civerolo E. L., Miller J. W., Stall R. E., Krass C. J., Poe S. R., DuCharme E. P. 1987; Citrus Canker in Florida. Plant Dis. 71:388–394
    [Google Scholar]
  22. Selander R. 1985; Protein polymorphism and the genetic structure of natural populations of bacteria. 85–106 In Ohta T., Aoki K. Population genetics and molecular evolution Japan Scientific Societies Press; Tokyo:
    [Google Scholar]
  23. Sneath P. H. A., Sokal R. R. 1973; Numerical taxonomy. The principles and practice of numerical classification W. H. Freeman & Co.; San Francisco:
    [Google Scholar]
  24. Stall R. E., Seymour C. P. 1983; Canker, a threat to citrus in the gulf-coast states. Plant Dis. 67:581–585
    [Google Scholar]
  25. Starr M. P. 1983; The genus Xanthomonas. 742–763 In Starr M. P. The prokaryotes: a handbook on habitats, isolation, and identification of bacteria Springer-Verlag; New York:
    [Google Scholar]
  26. Swings J., De Vos P., Van Den Mooter M., De Ley J. 1983; Transfer of Pseudomonas maltophilia Hugh 1981 to the genus Xanthomonas as Xanthomonas maltophilia (Hugh 1981) comb, nov. Int. J. Syst. Bacteriol. 33:409–413
    [Google Scholar]
  27. Thaveechai N., Schaad N. W. 1986; Serological and electrophoretic analysis of a membrane protein extract of Xanthomonas campestris pv. campestris from Thailand. Phytopathology 76:139–147
    [Google Scholar]
  28. Wishart D. 1987; Clustan user manual. , 4th. Computing Laboratory University of St. Andrews; Fife, Scotland:
    [Google Scholar]
  29. Yuen G. Y., Alvarez A. M., Benedict A. A., Trotter K. J. 1987; Use of monoclonal antibodies to monitor the dissemination of Xanthomonas campestris pv campestris. Phytopathology 77:366–370
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-39-1-14
Loading
/content/journal/ijsem/10.1099/00207713-39-1-14
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error