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Volume 165, Issue 6

Sialidase and N-acetylneuraminate catabolism in nutrition of Mycoplasma alligatoris Free

Abstract

The contribution of N-acetylneuraminate scavenging to the nutrition of Mycoplasma alligatoris was examined. The wild-type grew substantially faster (P<0.01) than the mutant strains that were unable either to liberate (extracellular NanI mutants) or to catabolize (NanA mutants) N-acetylneuraminate from glycoconjugates in minimal SP-4 medium supplemented only with serum, but the growth of sialidase-negative mutants could not be restored to wild-type rate simply by adding unconjugated sialic acid to the culture medium. In 1 : 1 growth competition assays the wild-type was recovered in >99-fold excess of a sialidase-negative mutant after co-culture on pulmonary fibroblasts in serum-free RPMI 1640 medium, even with supplemental glucose. The advantage of nutrient scavenging via this mechanism in a complex glycan-rich environment may help to balance the expected selective disadvantage conferred by the pathogenic effects of mycoplasmal sialidase in an infected host.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): growth , Mycoplasma , nutrition and sialidase
© 2019 The Authors
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2018-11-13
2024-04-24
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Sialidase and N-acetylneuraminate catabolism in nutrition of Mycoplasma alligatoris
Microbiology 165, 662 (2019); https://doi.org/10.1099/mic.0.000739
/content/journal/micro/10.1099/mic.0.000739
/content/journal/micro/10.1099/mic.0.000739
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