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Volume 162, Issue 7

Editor's Choice Functional genomics in identified a novel streptomycin resistance gene located in a hypervariable genomic region Free

Abstract

Numerous aminoglycoside resistance genes have been reported in spp. often resembling those from Gram-positive bacterial species and located in transferable genetic elements with other resistance genes. We discovered a new streptomycin (STR) resistance gene in showing 27–34 % amino acid identity to aminoglycoside 6-nucleotidyl-transferases described previously in . STR resistance was verified by gene expression and insertional inactivation. This -like gene differs from the previously described aminoglycoside resistance genes in spp. in several aspects. It does not appear to originate from Gram-positive bacteria and is located in a region corresponding to a previously described hypervariable region 14 of with no other known resistance genes detected in close proximity. Finally, it does not belong to a multiple drug resistance plasmid or transposon. This novel -like gene appears widely spread among as it is found in strains originating both from Europe and the United States and from several, apparently unrelated, hosts and environmental sources. The closest homologue (60 % amino acid identity) was found in certain and strains in a similar genomic location, but an association with STR resistance was not detected. Based on the findings presented here, we hypothesize that -like gene A has originated from a common ancestral proto-resistance element in spp., possibly encoding a protein with a different function. In conclusion, whole genome sequencing allowed us to fill in a knowledge gap concerning STR resistance in by revealing a novel STR resistance gene possibly inherent to .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): aminoglycoside , antimicrobial , Campylobacter , resistance and streptomycin
© 2016 The Authors
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2016-07-01
2024-04-18
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Functional genomics in Campylobacter coli identified a novel streptomycin resistance gene located in a hypervariable genomic region
Microbiology 162, 1157 (2016); https://doi.org/10.1099/mic.0.000304
/content/journal/micro/10.1099/mic.0.000304
/content/journal/micro/10.1099/mic.0.000304
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