- Volume 66, Issue 8, 2016
Volume 66, Issue 8, 2016
- New taxa
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- Proteobacteria
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Oblitimonas alkaliphila gen. nov., sp. nov., in the family Pseudomonadaceae, recovered from a historical collection of previously unidentified clinical strains
Eight Gram-stain-negative bacteria (B4199T, C6819, C6918, D2441, D3318, E1086, E1148 and E5571) were identified during a retrospective study of unidentified strains from a historical collection held in the Special Bacteriology Reference Laboratory at the Centers for Disease Control and Prevention. The strains were isolated from eight patients: five female, two male and one not specified. No ages were indicated for the patients. The sources were urine (3), leg tissue (2), foot wound, lung tissue and deep liver. The strains originated from seven different states across the USA [Colorado, Connecticut (2), Indiana, North Carolina, Oregon and Pennsylvania]. The strains grew at 10–42 °C, were non-motile, alkalitolerant, slightly halophilic, microaerophilic, and catalase- and oxidase-positive. The DNA G+C content was 47.3–47.6 mol%. The major cellular fatty acids were tetradecanoic acid (C14 : 0), hexadecanoic acid (C16 : 0) and 11-octadecenoic acid (C18 : 1ω7c). Polar lipids detected were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and unknown phospholipids; the only respiratory quinone detected was the ubiquinone Q-9 (100 %). 16S rRNA gene sequence analysis produced results with 95.6 % similarity to Pseudomonas caeni DSM 24390T and 95.2 % similarity to Thiopseudomonas denitrificans X2T. The results of the biochemical, chemotaxonomic and phylogenetic analyses between the study strains and some related type strains indicated that these strains represent a novel species of a new genus within the family Pseudomonadaceae , for which the name Oblitimonas alkaliphila gen. nov., sp. nov. is proposed. The type strain is B4199T (=DSM 100830T=CCUG 67636T).
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Bradyrhizobium stylosanthis sp. nov., comprising nitrogen-fixing symbionts isolated from nodules of the tropical forage legume Stylosanthes spp.
The introduction of legumes and nitrogen-fixing bacteria in tropical areas under pasture is a key factor for improvement of soil fertility. However, there are still very few studies concerning the symbionts of tropical forage legumes. We performed a polyphasic study with three strains representing the genus Bradyrhizobium (BR 446T, BR 510 and BR 511) isolated from the tropical perennial forage legume of the genus Stylosanthes. On the basis of 16S rRNA gene sequences, the three strains showed highest similarity with B. huanghuaihaiense , and in the analysis of the intergenic transcribed spacer (ITS) they showed less than 93.4 % similarity to all described species of the genus Bradyrhizobium . Multilocus sequence analysis (MLSA) with three, four or five (dnaK, glnII, gyrB, recA and rpoB) housekeeping genes confirmed that the BR strains belong to a distinct clade, with <96.5 % nucleotide identity with other members of the genus Bradyrhizobium . Average nucleotide identity (ANI) of genome sequences between strain BR 446T and B. huanghuaihaiense was below the threshold for species circumscription (90.7 %). DNA-DNA hybridization resulted in ΔT m values over 6.7 °C with the most closely related species. Similarities among the BR strains and differences from other species were confirmed by rep-PCR analysis. Interestingly, the BR strains were grouped in the analysis of nifH and nodC genes, but showed higher similarity with B. iriomotense and B. manausense than with B. huanghuaihaiense , indicating a different evolutionary history for nitrogen-fixation genes. Morpho-physiological, genotypic and genomic data supported that these BR strains represent a novel species for which the name Bradyrhizobium stylosanthis sp. nov. is suggested. The type strain is BR 446T (=CNPSo 2823T=HAMBI 3668T=H-8T), isolated from Stylosanthes guianensis.
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Agarivorans aestuarii sp. nov., an agar-degrading bacterium isolated from a tidal flat
More LessA Gram-reaction-negative, aerobic, non-spore forming, rod-shaped bacterium motile with a single polar flagellum, designated strain hydD622T, was isolated from the sediment of a tidal flat at Asan Bay, Korea. Strain hydD622T exhibited an agarolytic activity. Comparison of 16S rRNA gene sequences revealed that strain hydD622T was closely related to Agarivorans litoreus KCTC 42116T, Agarivorans albus KCTC 22256T and Agarivorans gilvus KCTC 32555T with similarities of 98.4, 98.0 and 96.5 %, respectively. Strain hydD622T was clustered distantly from the other genera in the family Alteromona daceae but formed a unique clade within the genus Agarivorans based on the 16S rRNA gene sequence. The DNA–DNA relatedness with Agarivorans litoreus KCTC 42116T and Agarivorans. albus KCTC 22256T was 39.0 and 37.8 %, respectively. The major fatty acids (>10 %) were C16 : 0,C16 : 1ω6c/C16 : 1ω7c and C18 : 1ω6c/C18 : 1ω7c. The respiratory quinone was ubiquinone-8, and the polar lipid profile consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and an unidentified lipid. The DNA G+C content was 44 mol%. On the basis of physiological, chemotaxonomic and phylogenetic analyses, strain hydD622T represents a novel species within the genus Agarivorans , for which the name Agarivorans aestuarii sp. nov. is proposed. The type strain of Agarivorans aestuarii sp. nov. is hydD622T (=KCTC 32543T=CGMCC 1.12692T).
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Pseudoruegeria aestuarii sp. nov., of the family Rhodobacteraceae, isolated from a tidal flat
A Gram-stain-negative, non-motile, aerobic and rod-shaped bacterium, designated strain MME-001T, was isolated from the tidal flat of Muui-do in the Republic of Korea. Phylogenetic trees based on the 16S rRNA gene sequence showed that strain MME-001T belonged to the genus Pseudoruegeria in the family Rhodobacteraceae and that it shared the highest 16S rRNA gene sequence similarity with Pseudoruegeria sabulilitoris GJMS-35T (98.0 % similarity of the 16S rRNA gene). Growth of strain MME-001T occurred in the presence of 1.0–7.0 % (w/v) NaCl at 15−40 °C and pH 7.0–9.0, with optimal growth in the presence of 2.0–3.0 % (w/v) NaCl at 25–30 °C and pH 7.0. Ubiquinone-10 was the major respiratory quinone. Major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and an unidentified lipid. The major cellular fatty acids were C18 : 1 ω7c and C16 : 0. The genomic DNA G+C content was 62 mol%. DNA-DNA hybridization values between strain MME-001T and P. sabulilitoris KCTC 42111T, ‘ Pseudoruegeria limi ’ KCTC 32460, Pseudoruegeria lutimaris KCTC 22690T, Pseudoruegeria aquimaris KCTC 12737T and Pseudoruegeriahaliotis KACC 17214T was 36±5, 57±7, 34±4, 18±5 and 21±3 %, respectively. Based on the phenotypic and phylogenetic taxonomical properties, this strain MME-001T represents a novel species of the genus Pseudoruegeria , for which the name Pseudoruegeria aestuarii sp. nov. is proposed. The type strain is MME-001T (=KCCM 43133T=JCM 30751T).
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Vibrio algivorus sp. nov., an alginate- and agarose-assimilating bacterium isolated from the gut flora of a turban shell marine snail
More LessAn agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium, designated strain SA2T, was isolated from the gut of a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan. The 16S rRNA gene sequence of strain SA2T was 99.59 % identical to that of Vibrio rumoiensis DSM 19141T and 98.19 % identical to that of Vibrio litoralis DSM 17657T. This suggested that strain SA2T could be a subspecies of V. rumoiensis or V. litoralis . However, DNA–DNA hybridization results showed only 37.5 % relatedness to DSM 19141T and 44.7 % relatedness to DSM 17657T, which was far lower than the 70 % widely accepted to define common species. Strain SA2T could assimilate agarose as a sole carbon source, whereas strains DSM 19141T and DSM 17657T could not assimilate it at all. Furthermore, results using API 20NE and API ZYM kits indicated that their enzymic and physiological phenotypes were also different. These results suggested that strain SA2T represented a novel species within the genus Vibrio . The major isoprenoid quinone in SA2T was Q-8, and its major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The major fatty acids were summed feature 3, (comprising C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0, and summed feature 8 (comprising C18 : 1ω6c and/or C18 : 1ω7c). The DNA G+C content of SA2T was 40.7 mol%. The name proposed for this novel species of the genus Vibrio is Vibrio algivorus sp. nov., with the type strain designated SA2T (=DSM 29824T=NBRC 111146T).
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Novosphingobium naphthae sp. nov., from oil-contaminated soil
More LessDuring the study of hydrocarbon-degrading bacteria in the oil-contaminated soil of Gunsan, North Jeolla Province, South Korea, a light-grey-pigmented, Gram-staining-negative, aerobic, non-motile and rod-shaped bacterium, designated strain D39T, was isolated. This strain was non-sporulating, catalase-negative and oxidase-positive. It was able to grow at 12–42 °C, pH 5.5–8.5 and with 0–1 % (w/v) NaCl. This strain was characterized taxonomically by a polyphasic approach. Based on the results of 16S rRNA gene sequence analysis, strain D39T belongs to the genus Novosphingobium and is closely related to ‘ Novosphingobium ginsenosidimutans' FW-6 (97.30 % sequence similarity), Novosphingobium mathurense SM117T (97.17 % sequence similarity) and Novosphingobium aquiterrae E-II-3T (97.01 % sequence similarity). The only respiratory quinone was ubiquinone-10 and the major polyamine was spermidine. The polar lipid profile revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid and phosphatidyl-N-methylethanolamine. The predominant fatty acids of strain D39T were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C17 : 1ω6c and C14 : 0 2-OH. The genomic DNA G+C content of this novel strain was 66.7 mol%. The DNA–DNA relatedness between strain D39T and ‘N. ginsenosidimutans' KACC 16615, N. mathurense KACC 14598T, N. aquiterrae KACC 17599T and Novosphingobium kunmingense DSM 25975T were 33.7 %, 29.0 %, 22.3 % and 18.3 %, respectively. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain D39T represents a novel species of the genus Novosphingobium , for which the name Novosphingobium naphthae sp. nov. is proposed. The type strain is D39T (=KEMB 9005-346T=KACC 18593T=JCM 31158T).
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Marinomonas gallaica sp. nov. and Marinomonas atlantica sp. nov., isolated from reared clams (Ruditapes decussatus)
More LessThree Gram-negative bacterial strains (Cmf 17.2T, Rd 20.33 and Cmf 18.22T) isolated from reared clams in Galicia were subjected to a taxonomic study, based on genetic and phenotypic characterization. Analysis of the 16S rRNA gene allowed the identification of the strains as members of the genus Marinomonas , sharing the highest similarity with Marinomonas aquimarina CECT 5080T (97.8 %–98.5 % 16S rRNA gene sequence similarity). Phylogenetic analysis of the sequences showed that the three isolates formed two different groups distantly related to their closest relative, M. aquimarina . DNA–DNA hybridizations were performed to confirm the taxonomic position and the results were below the recommended threshold for species delimitation, specifically 44.5 % (Cmf 17.2T with M. aquimarina CECT 5080T) and 55 % (Cmf 18.22Twith M. aquimarina CECT 5080T). Furthermore, the average nucleotide identity (ANIb, ANIm and OrthoANI) and in silico estimated DNA–DNA reassociation values among Cmf 17.2T, Cmf 18.22T and M. aquimarina CECT 5080T were in all cases below the respective threshold for species differentiation. The estimated G+C content of the genomic DNA was found to be 45.3 % (Cmf 17.2T) and 44.6 % (Cmf 18.22T). The principal fatty acids of the strains were found to be summed feature 3 (C16 : 1 ω7c/C16 : 1ω6c), summed feature 8 (C18 : 1ω7c/C18 : 1ω6c), C16 : 0, C12 : 0 and C10 : 0 3-OH. The results obtained on the characterization of the clam isolates indicate that they represent two novel species of the genus Marinomonas, for which the names Marinomonas gallaica sp. nov. (type strain Cmf 17.2T=CECT 9049T=LMG 29243T) and Marinomonas atlantica sp. nov. (type strain Cmf 18.22T=CECT 9050T=LMG 29244T) are proposed.
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Cribrihabitans pelagius sp. nov., a marine alphaproteobacterium isolated from seawater
More LessA Gram-stain-negative, strictly aerobic, cream-coloured, motile, chemoheterotrophic, rod-shaped bacterium, designated strain KMU-32T, was isolated from seawater at Najeong Beach in Korea. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the novel isolate was affiliated with the family Rhodobacteraceae of the class Alphaproteobacteria and that it showed highest sequence similarity (98.2 %) to Cribrihabitans neustonicus CC-AMHB-3T. The DNA–DNA relatedness value between strain KMU-32T and C. neustonicus CC-AMHB-3T was 48.5 ± 4 %. The DNA G+C content of strain KMU-32T was determined to be 63.7 mol%. Ubiquinone 10 (Q-10) was the sole respiratory quinone. The predominant cellular fatty acid was C18 : 1 ω7c. Strain KMU-32T had diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified aminolipid and an unidentified lipid as polar lipids. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, strain KMU-32T is considered to represent a novel species of the genus Cribrihabitans , for which the name Cribrihabitans pelagius sp. nov. is proposed. The type strain is KMU-32T (= KCTC 42981T = NBRC 111834T).
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Allohahella marinimesophila gen. nov., sp. nov., isolated from seawater and reclassification of Hahella antarctica as Allohahella antarctica comb. nov.
More LessA Gram-stain-negative, non-endospore-forming, strictly aerobic, irregular rod-shaped bacterium without flagellum, designated strain H94T, was isolated by the high-throughput cultivation method from seawater of an amphioxus breeding zone in the coastal region of Qingdao, China. Growth was observed at 4–37 °C (optimum 28 °C), at pH 6.0–10.0 (optimum pH 7.0) and in the presence of 1–12 % (w/v) NaCl (optimum 1–2 %). The predominant cellular fatty acids were C18 : 1 ω9c, C16 : 0 and C16 : 1 ω9c. The polar lipids comprised phosphatidylethanolamine, phosphatidylglycerol and an unidentified phosphoglycolipid. The major respiratory quinone was ubiquinone-9 (Q-9). The genomic DNA G+C content of strain H94T was 56.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain H94T shared the highest similarity (95.9 %) with Hahella antarctica NBRC 102683T, and exhibited 92.9 % and 92.1 % similarity with the two other recognized Hahella species, Hahella chejuensis KCTC 2396T and Hahella ganghwensis DSM 17046T, respectively. The phylogenetic position revealed that strain H94T formed a stable distinct lineage cluster together with Hahella antarctica NBRC 102683T and this result was further confirmed by multilocus sequence analysis based on housekeeping genes gyrB and rpoB. On the basis of the polyphasic taxonomic analyses, strain H94T is considered to represent a novel species in a new genus, for which the name Allohahella marinimesophila gen. nov., sp. nov. is proposed. The type strain of Allohahella marinimesophila is H94T (=CGMCC 1.10800T=JCM 17555T). It is also proposed that Hahella antarctica should be reclassified within the genus Allohahella as Allohahella antarctica comb. nov. (type strain NBRC 102683T=IMCC 3113T=KCCM 42675T). The type species of the genus Allohahella is Allohahella antarctica comb. nov.
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- Evolution, Phylogeny and Biodiversity
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The small subunit rRNA gene sequence of the chonotrich Chilodochona carcini Jankowski, 1973 confirms chonotrichs as a dysteriid-derived clade (Phyllopharyngea, Ciliophora)
More LessThe chonotrichs are sessile ciliated protozoa that are ectosymbiotic on the body parts of a variety of crustaceans. They have long been considered a separate group because their sessile habit has resulted in the evolution of a very divergent body form and reproductive strategy compared to free-living ciliates. In the mid-20th Century, the free-living dysteriid cyrtophorian ciliates were proposed as a potential sister clade because the chonotrich bud or daughter cell showed similarities during division morphogenesis (i.e. ontogeny) to these free-living dysteriids. A single small subunit (SSU) rRNA gene sequence is available for the chonotrich Isochona sp. However, its authenticity has recently been questioned, and the placement of this sequence within the dysteriid clade has added to this controversy. In this report, the SSUrRNA gene sequence of the chonotrich Chilodochona carcini, ectosymbiotic on the green crab Carcinus maenas, is provided. Topology testing of the SSUrRNA gene phylogeny, constructed by Bayesian Inference, robustly supports the sister-group relationship of Isochona sp. and Chilodochona carcini, the monophyly of these two chonotrichs, and the divergence of the chonotrich clade within the dysteriid clade.
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Emended description of the family Beijerinckiaceae and transfer of the genera Chelatococcus and Camelimonas to the family Chelatococcaceae fam. nov.
More LessThe family Beijerinckiaceae was circumscribed in 2005 to accommodate four genera of phylogenetically related alphaproteobacteria: Beijerinckia , Chelatococcus , Methylocella and Methylocapsa . Later, four additional genera, i.e. Methylovirgula , Methyloferula , Methylorosula and Camelimonas , were described and assigned to this family, which now accommodates 21 species with validly published names. Members of this family possess strikingly different lifestyles, including chemoheterotrophy, facultative methylotrophy, obligate methanotrophy and facultative methanotrophy. Levels of 16S rRNA gene sequence similarity among most of these bacteria range from 96 to 98 %, suggesting a common evolutionary origin. The genera Chelatococcus and Camelimonas , however, are not monophyletic with the other described genera based on 16S rRNA gene sequence phylogeny, and instead form a distant cluster more closely related to the Methylobacteriaceae . Physiologically these two genera also lack several properties common to the other Beijerinckiaceae . On the other hand, the genus Rhodoblastus , presently considered a member of the Bradyrhizobiaceae , affiliates with high confidence to the Beijerinckiaceae . Here, we propose to transfer the genera Chelatococcus and Camelimonas to the family Chelatococcaceae fam. nov., and present an emended description of the family Beijerinckiaceae , including the genus Rhodoblastus .
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- Taxonomic Note
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Genomic evidence that Vibrio inhibens is a heterotypic synonym of Vibrio jasicida
More LessVibrio inhibens is a marine bacterium species of the genus Vibrio ( Vibrionaceae , Gammaproteobacteria ). The species has been shown to be closely related to members of the genus Vibrio in the so-called Harveyi clade. The clade includes at least 11 closely related species with similar physiological and biochemical properties. Due to these similarities, species of the Harveyi clade are difficult to characterize taxonomically. Previously phenotypic and genotypic properties of the V. inhibens type strain were compared with six species of the Harveyi clade, resulting in the possibility that V. inhibens could be a synonym of a previously described species. In this study, the taxonomic status of V. inhibens was analyzed using genomic approaches. The whole-genome sequence of the type strain of V. inhibens , CECT 7692T, was obtained and analyzed. Calculations of average nucleotide identity with the blast algorithm (ANIb) showed that CECT 7692T has an ANIb of 97.5 % or higher to five strains of Vibrio. jasicida , including the type strain, but an ANIb lower than 93.5 % to other members of the Harveyi clade Vibrio . Phylogenetic analysis based on nucleotide sequences of 133 protein-coding genes showed a close evolutionary relationship of CECT 7692T to V. jasicida . Based on these results, Vibrio inhibens is proposed to be a later heterotypic synonym of V. jasicida .
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- ICSP Matters
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The status of the species Moorella thermoautotrophica Wiegel et al. 1981. Request for an Opinion
More LessBased on the results of DNA–DNA hybridization and 16S rRNA gene sequence analyses, it was ascertained that the type strain of Moorella thermoautotrophica does not exist in any established culture collection or with the authors who originally described this species. Therefore, this species cannot be included in any further scientific studies. It is proposed that the Judicial Commission place the name Moorella thermoautotrophica on the list of rejected names if a suitable type strain is not found or a neotype is not proposed within two years following the publication of this Request for an Opinion.
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Volumes and issues
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Volume 74 (2024)
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Volume 73 (2023)
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Volume 72 (2022 - 2023)
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Volume 71 (2020 - 2021)
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Volume 70 (2020)
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Volume 69 (2019)
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Volume 68 (2018)
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Volume 67 (2017)
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Volume 66 (2016)
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Volume 65 (2015)
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Volume 64 (2014)
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Volume 63 (2013)
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Volume 62 (2012)
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Volume 61 (2011)
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Volume 60 (2010)
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Volume 59 (2009)
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Volume 58 (2008)
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Volume 57 (2007)
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Volume 56 (2006)
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Volume 55 (2005)
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Volume 54 (2004)
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Volume 53 (2003)
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Volume 52 (2002)
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Volume 51 (2001)
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Volume 50 (2000)
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Volume 49 (1999)
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Volume 48 (1998)
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Volume 47 (1997)
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Volume 46 (1996)
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Volume 45 (1995)
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Volume 44 (1994)
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Volume 43 (1993)
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Volume 42 (1992)
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Volume 41 (1991)
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Volume 40 (1990)
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Volume 39 (1989)
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Volume 38 (1988)
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Volume 37 (1987)
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Volume 36 (1986)
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Volume 35 (1985)
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Volume 34 (1984)
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Volume 33 (1983)
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Volume 32 (1982)
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Volume 31 (1981)
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Volume 30 (1980)
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Volume 29 (1979)
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Volume 28 (1978)
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Volume 27 (1977)
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Volume 26 (1976)
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Volume 25 (1975)
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Volume 24 (1974)
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Volume 23 (1973)
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Volume 22 (1972)
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Volume 21 (1971)
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Volume 20 (1970)
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Volume 19 (1969)
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Volume 18 (1968)
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Volume 17 (1967)
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Volume 16 (1966)
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Volume 15 (1965)
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Volume 14 (1964)
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Volume 13 (1963)
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Volume 12 (1962)
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Volume 11 (1961)
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Volume 10 (1960)
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Volume 9 (1959)
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Volume 8 (1958)
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Volume 7 (1957)
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Volume 6 (1956)
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Volume 5 (1955)
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Volume 4 (1954)
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Volume 3 (1953)
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Volume 2 (1952)
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Volume 1 (1951)