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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 74, Issue 4

sp. nov., a novel marine actinobacterium isolated from deep-sea sediment of Madeira Archipelago, Portugal No Access

Abstract

A novel strain, MA3_2.13, was isolated from deep-sea sediment of Madeira Archipelago, Portugal, and characterized using a polyphasic approach. This strain produced dark brown soluble pigments, bronwish black substrate mycelia and an aerial mycelium with yellowish white spores, when grown on GYM 50SW agar. The main respiratory quinones were MK-10(H), MK-10(H) and MK-10(H). Diphosphatidylglycerol, phosphatidylethanolamine, three unidentified phospholipids and two glycophospholipids were identified as the main phospholipids. The major cellular fatty acids were iso-C, iso-C, anteiso-C and anteiso-C. Phylogenetic analyses based on 16S rRNA gene showed that strain MA3_2.13 is a member of the genus and was most closely related to NEAU-YY642 (NR_180032.1; 16S rRNA gene similarity 97.9 %), YIM 65188 (NR_044582.1; 16S rRNA gene similarity 97.4 %), 3MP-10 (NR_170412.1; 16S rRNA gene similarity 97.3 %) and NEAU-LZS-5 (NR_133874.1; 16S rRNA gene similarity 97.0 %). Genome pairwise comparisons with closest related type strains retrieved values below the threshold for species delineation suggesting that strain MA3_2.13 represents a new branch within the genus s. Based on these results, strain MA3_2.13 (=DSM 115980=LMG 33094) is proposed as the type strain of a novel species of the genus , for which the name sp. nov. is proposed.

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Keyword(s): polyphasic analysis , Actinomycetota , deep-sea sediment , phylogenetic analysis , phylogenomic analysis and Streptomyces
© 2024 The Authors
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2024-04-19
2024-05-02
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Streptomyces profundus sp. nov., a novel marine actinobacterium isolated from deep-sea sediment of Madeira Archipelago, Portugal
Int J Syst Evol Microbiol 74, 006341 (2024); https://doi.org/10.1099/ijsem.0.006341
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