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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 74, Issue 4

sp. nov., a slightly halophilic bacterium isolated from an enrichment system with saline lake sediment No Access

Abstract

A Gram-stain-negative, non-motile, and slightly halophilic alphaproteobacterium, designated strain EGI FJ00035, was isolated from enrichment sediment samples of a saline lake in Xinjiang Uygur Autonomous Region, PR China. The taxonomic position of the isolate was determined using the polyphasic taxonomic and phylogenomic analyses. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain EGI FJ00035 formed a distinct clade with ‘’ UJN715 and ‘’ lm93 with sequence similarities of 98.44 and 98.22 %, respectively, while sharing less than 96.7 % with other valid type strains. The novel isolate could be distinguished from other species of the genus by its distinct phenotypic, physiological, and genotypic characteristics. Optimal growth of strain EGI FJ00035 occurred on marine agar 2216 at pH 7.0 and 30 °C. The major respiratory quinone was Q-10, while the major fatty acids (>5 %) were C cyclo ω8, summed feature 8 (C ω6 and/or C ω7), C, C, and iso-C. The detected polar lipids included diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, unidentified aminophospholipids, unidentified glycolipids, and an unidentified lipid. Based on its genome sequence, the G+C content of strain EGI FJ00035 was 63.2 mol%. The average nucleotide identity, average amino acid identity, and digital DNA–DNA hybridization values of strain EGI FJ00035 against related members of the genus were below the thresholds for delineation of a novel species. According our polyphasic taxonomic data, strain EGI FJ00035 represents a new species of the genus , for which the name sp. nov. is proposed. The type strain of the proposed novel isolate is EGI FJ00035 (=KCTC 92251=CGMCC 1.19480).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Barkol Lake , Chelativorans , Chelativorans salis sp. nov. and polyphasic taxonomy
Funding
This study was supported by the:
  • Xinjiang Uygur Autonomous Region regional coordinated innovation project (Award 2020E01047 and 2021E01018)
    • Principle Award Recipient: Wen-JunLi
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2021FY100900)
    • Principle Award Recipient: Bao-zhuFang
  • The Third Xinjiang Scientific Expedition Program (Award 2022xjkk1200)
    • Principle Award Recipient: Hong-chenJiang
© 2024 Sun Yat-Sen University
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Chelativorans salis sp. nov., a slightly halophilic bacterium isolated from an enrichment system with saline lake sediment
Int J Syst Evol Microbiol 74, 006340 (2024); https://doi.org/10.1099/ijsem.0.006340
/content/journal/ijsem/10.1099/ijsem.0.006340
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