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Volume 74, Issue 4

sp. nov., a pathogen causing bacterial soft rot of Japanese angelica tree in Japan Open Access

Abstract

Phytopathogenic bacteria (MAFF 302110 and MAFF 302107) were isolated from lesions on Japanese angelica trees affected by bacterial soft rot in Yamanashi Prefecture, Japan. The strains were Gram-reaction-negative, facultatively anaerobic, motile with peritrichous flagella, rod-shaped, and non-spore-forming. The genomic DNA G+C content was 51.1 mol % and the predominant cellular fatty acids included summed feature 3 (C ω7 and/or C ω6), C, summed feature 8 (C ω7 and/or C ω6), summed feature 2 (comprising any combination of C aldehyde, an unknown fatty acid with an equivalent chain length of 10.928, C iso I, and C 3OH), and C. Phylogenetic analyses based on 16S rRNA and gene sequences, along with phylogenomic analysis utilizing whole-genome sequences, consistently placed these strains within the genus . However, their phylogenetic positions did not align with any known species within the genus. Comparative studies involving average nucleotide identity and digital DNA–DNA hybridization with the closely related species indicated values below the thresholds employed for the prokaryotic species delineation (95–96 % and 70 %, respectively), with the highest values observed for DPMP315 (92.10 and 47.1 %, respectively). Phenotypic characteristics, cellular fatty acid composition, and a repertoire of secretion systems could differentiate the strains from their closest relatives. The phenotypic, chemotaxonomic, and genotypic data obtained in this study show that MAFF 302110/MAFF 302107 represent a novel species of the genus , for which we propose the name sp. nov., designating MAFF 302110 (=ICMP 25161) as the type strain.

  • Received:
  • Accepted:
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Keyword(s): Aralia elata , bacterial soft rot disease , Erwinia carotovora , Japanese angelica tree , Japanese aralia and Pectobacterium carotovorum
Funding
This study was supported by the:
  • Environmental Restoration and Conservation Agency (Award S18-2)
    • Principle Award Recipient: NobutakaSomeya
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-04-16
2024-04-29
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Pectobacterium araliae sp. nov., a pathogen causing bacterial soft rot of Japanese angelica tree in Japan
Int J Syst Evol Microbiol 74, 006326 (2024); https://doi.org/10.1099/ijsem.0.006326
/content/journal/ijsem/10.1099/ijsem.0.006326
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