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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 74, Issue 4

gen. nov., sp. nov., the first oceanic methanogen, isolated from potential methane hydrate bearing area offshore southwestern Taiwan No Access

Abstract

A novel mesophilic, hydrogenotrophic methanogen, strain CWC-04, was obtained from a sediment sample extracted from a gravity core retrieved at station 22 within the KP-9 area off the southwestern coast of Taiwan during the ORIII-1368 cruise in 2009. Cells of strain CWC-04 were rod-shaped, 1.4–2.9 µm long by 0.5–0.6 µm wide, and occurred singly. Strain CWC-04utilized formate, H/CO, 2-propanol/CO or 2-butanol/CO as catabolic substrates. The optimal growth conditions were 42 °C, 0.17 M NaCl and pH 5.35. The genomic DNA G+C content calculated from the genome sequence of strain CWC-04 was 46.19 mol%. Phylogenetic analysis of 16S rRNA gene revealed that strain CWC-04 is affiliated with the genus . The 16S rRNA gene sequences similarities within strains MRE50, SANAE and HZ254 were 93.7, 93.0 and 91.3 %, respectively. In addition, the optical density of CWC-04 culture dropped abruptly upon entering the late-log growth phase, with virus-like particles (150 nm in diameter) being observed on and around the cells. This observation suggests that strain CWC-04 harbours a lytic virus. Based on these phenotypic, phylogenetic and genomic results, we propose that strain CWC-04 represents a novel species of a novel genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain is CWC-04 (=BCRC AR10050=NBRC 113165).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Archaea , marine sediment , methane hydrate , methanogen and Methanooceanicella
Funding
This study was supported by the:
  • Sanming University, Sanming, Fujian (Award 20YG09)
    • Principle Award Recipient: ChenSheng-Chung
  • Ministry of Science and Technology, Taiwan (Award MOST103/104/105/106/107-3113-M-005-001)
    • Principle Award Recipient: Mei-ChinLai
  • Central Geological Survey, Ministry of Economic Affairs (Award 99-5226904000-04-03)
    • Principle Award Recipient: Mei-ChinLai
© 2024 The Authors
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Methanooceanicella nereidis gen. nov., sp. nov., the first oceanic Methanocellaceae methanogen, isolated from potential methane hydrate bearing area offshore southwestern Taiwan
Int J Syst Evol Microbiol 74, 006322 (2024); https://doi.org/10.1099/ijsem.0.006322
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