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Volume 72, Issue 5

sp. nov., isolated from rhizosphere of plant with antioxidative potential No Access

Abstract

A novel actinomycete strain, JA03, belonging to the genus , was isolated from the rhizosphere of (L.) Spreng. It was characterized taxonomically using a polyphasic approach. It grew at 25–37 °C, at pH 5–10 and with 6 % (w/v) NaCl. It contained -diaminopimelic acid in the cell-wall peptidoglycan. Ribose and glucose were detected in its whole-cell hydrolysate. The predominant cellular fatty acids were iso-C, anteiso-C, C, iso-C and iso-C. Detected polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, unidentified phospholipids and unidentified amino lipids. Based on the results of 16S rRNA gene sequence analyses, strain JA03 showed highest similarity to NBRC 12860 (98.76 %), TW1S1 (98.69 %) and 172115 (98.68 %). Strain JA03 has a genome size of 9 092 851 bp with DNA G+C content of 71.28 mol%. The average nucleotide identity (ANI)- and ANI-MUMmer values of strain JA03 and related type strains were 79.6–89.2 and 86.7–92.5 %, respectively, and the digital DNA–DNA hybridization values were 27.3–46.4 %. Ethyl acetate extract of JA03 exhibited total phenolic content (33.4±0.6 µg mg gallic acid equivalent), ferric reducing power value (70.8±1.8 µg mg ascorbic acid equivalent) and 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity (IC=67.0±21.1 µg ml). Intracellular reactive oxygen species and NO production in RAW264.7 macrophage cells induced by HO and lipopolysaccharide were inhibited with IC of 67.40 and 16.95 µg ml, respectively. Based on the taxonomic results, it has been concluded that strain JA03 represents a novel species of the genus for which the name sp. nov., is proposed. The type strain is JA03 (=LMG 32415=TISTR 2999).

  • Received:
  • Accepted:
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Keyword(s): antioxidant , polyphasic taxonomy , rhizosphere and Streptomyces barringtoniae
Funding
This study was supported by the:
  • Ratchadaphiseksomphot Endowment Fund
    • Principle Award Recipient: KhaingZar Wai
© 2022 The Authors
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2022-05-18
2024-04-24
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Streptomyces barringtoniae sp. nov., isolated from rhizosphere of plant with antioxidative potential
Int J Syst Evol Microbiol 72, 005364 (2022); https://doi.org/10.1099/ijsem.0.005364
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