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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 2

Nonomuraea lactucae sp. nov., a novel actinomycete isolated from rhizosphere soil of lettuce (Lactuca sativa) Free

Abstract

A novel actinobacterium, designated strain NEAU-YG30, was isolated from the rhizosphere soil of lettuce collected in Heilongjiang province, north-east China. The taxonomic position of the strain was investigated using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain NEAU-YG30 belongs to the genus Nonomuraea , and shared highest sequence similarity to Nonomuraea muscovyensis KCTC 29233 (97.9 %) and Nonomuraea indica CCTCC AA 209050 (97.6 %). The major menaquinone was identified as MK-9(H4). The major fatty acids were 10-methyl C17 : 0, C17 : 0, iso-C16 : 0 2-OH, iso-C16 : 0 and C16 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxyl-phosphatidylethanolamine, phosphatidylinositol, an unidentified phospholipid and three unidentified lipids. The genomic DNA G+C content of strain NEAU-YG30 was 70.5 mol%. DNA–DNA hybridization values between them were less than 70 %. On the basis of phenotypic, genotypic and phylogenetic data, strain NEAU-YG30 can be characterized to represent a novel species of the genus Nonomuraea , for which the name Nonomuraea lactucae sp. nov. is proposed. The type strain is NEAU-YG30 (=JCM 32549=CGMCC 4.7506).

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Keyword(s): 16S rRNA gene , lettuce , Nonomuraea lactucae sp. nov. , polyphasic taxonomy and rhizosphere soil
© 2019 IUMS
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2018-12-12
2024-04-23
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Nonomuraea lactucae sp. nov., a novel actinomycete isolated from rhizosphere soil of lettuce (Lactuca sativa)
Int J Syst Evol Microbiol 69, 316 (2019); https://doi.org/10.1099/ijsem.0.003113
/content/journal/ijsem/10.1099/ijsem.0.003113
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