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Volume 68, Issue 8

Lelliottia aquatilis sp. nov., isolated from drinking water Free

Abstract

Five beige-pigmented, oxidase-negative bacterial isolates, 6331-17, 6332-17, 6333-17, 6334-17 and 9827-07, isolated either from a drinking water storage reservoir or drinking water in 2006 and 2017 in Germany, were examined in detail applying by a polyphasic taxonomic approach. Cells of the isolates were rod-shaped and Gram-stain-negative. Comparison of the 16S rRNA gene sequences of these five isolates showed highest sequence similarities to Lelliottia amnigena (99.98 %) and Lelliottia nimipressuralis (99.99 %). Multilocus sequence analyses based on concatenated partial rpoB, gyrB, infB and atpD sequences confirmed the clustering of these isolates with Lelliottia species, but also revealed a clear distinction to the closest related type strains. Analysis of the genome sequences of these isolates indicated >70 % in silico DNA–DNA hybridization and high average nucleotide identities between strains. Nevertheless, they showed only <70 and <95 % similarity to the type strains of these two Lelliottia species. The fatty acid profiles of these isolates were very similar and consisted of the major fatty acids C16:0, C17 : 0cyclo, C15 : 0iso 2-OH/C16 : 1ω7c and C18 : 1 ω7c. In addition, physiological/biochemical tests revealed high phenotypic similarity to each other. These cumulative data indicate that these isolates represent a novel Lelliottia species, for which the name Lelliottia aquatilis sp. nov. is proposed, with strain 6331-17 (=CCM 8846=CIP 111609=LMG 30560) as the type strain.

  • Received:
  • Accepted:
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Keyword(s): ANI , isDDH , Lelliottia , MLSA and rep-PCRs
© 2018 IUMS
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2018-06-22
2024-04-19
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Lelliottia aquatilis sp. nov., isolated from drinking water
Int J Syst Evol Microbiol 68, 2454 (2018); https://doi.org/10.1099/ijsem.0.002854
/content/journal/ijsem/10.1099/ijsem.0.002854
/content/journal/ijsem/10.1099/ijsem.0.002854
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