1887

Abstract

A Gram-staining-negative, strictly aerobic, transparent and smooth, curved-rod-shaped motile bacterium with a single polar flagellum, designated strain sw100, was isolated from surface seawater of South Pacific Gyre during Integrated Ocean Drilling Program Expedition 329. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain sw100 was most closely related to the member of the family and was distinct from the most closely related genera , and (95.8, 94.5–95.3 and 94.4 % 16S rRNA gene sequence similarities, respectively). Growth occurred in the presence of 0–11 % NaCl (w/v, optimum 7 %), at 10–37 °C (optimum 28 °C) and pH 6.0–9.0 (optimum pH 7.0). The major fatty acids (>10 % of total fatty acids) are C, C N alcohol and summed feature 3 (C 6 and/or C 7). The major polar lipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unidentified polar lipids. The DNA G+C content of strain sw100 was 55.3 mol%. The major respiratory quinone was ubiquinone-10 (Q-10). On the basis of polyphasic analyses, strain sw100 is considered to represent a novel species in a novel genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is sw100 (=JCM 30597=MCCC 1K00501=KCTC 42424).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001461
2016-12-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/12/5010.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001461&mimeType=html&fmt=ahah

References

  1. Ausubel F. M., Bren R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. (editors) 1995 Short Protocols in Molecular Biology: A Compendium of Methods From Current Protocols in Molecular Biology, 3rd edn.. New York: Wiley;
    [Google Scholar]
  2. Bernardet J. F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  3. Beveridge T. J., Lawrence J. R., Murray R. G. E. 2007; Sampling and staining for light microscopy. In Methods for General and Molecular Microbiology, 3rd edn. pp 19–33 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  4. Bowditch R. D., Baumann L., Baumann P. 1984; Description of Oceanospirillum kriegii sp. nov. and O. jannaschii sp. nov. and assignment of two species of Alteromonas to this genus as O. commune comb. nov. and O. vagum comb. nov. Curr Microbiol 10:221–229 [CrossRef]
    [Google Scholar]
  5. Choi A., Cho J. C. 2013; Thalassolituus marinus sp. nov., a hydrocarbon-utilizing marine bacterium. Int J Syst Evol Microbiol 63:2234–2238 [View Article][PubMed]
    [Google Scholar]
  6. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  7. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  8. Hsu S. C., Lockwood J. L. 1975; Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Appl Microbiol 29:422–426[PubMed]
    [Google Scholar]
  9. Hylemon P. B., Wells Jr J. S., Krieg N. R., Jannasch H. W. 1973; The genus Spirillum: a taxonomic study. Int J Syst Evol Microbiol 23:340–380 [View Article]
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  11. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  12. Kloos K., Munch J. C., Schloter M. 2006; A new method for the detection of alkane-monooxygenase homologous genes (alkB) in soils based on PCR-hybridization. J Microbiol Methods 66:486–496 [View Article][PubMed]
    [Google Scholar]
  13. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [CrossRef]
    [Google Scholar]
  14. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  15. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  16. Moore E. R. B., Arnscheidt A., Kruger A., Strömpl C., Mau M. 1999; Simplified protocols for the preparation of genomic DNA from bacterial cultures. In Molecular Microbial Ecology Manual pp 1.6.1.1–1.6.1.15 Edited by Akkermans A. D. L., van Elsas J. D., de Bruijn F. J. Kluwer: Dordrecht;
    [Google Scholar]
  17. Ostle A. G., Holt J. G. 1982; Nile blue a as a fluorescent stain for poly-beta-hydroxybutyrate. Appl Environ Microbiol 44:238–241[PubMed]
    [Google Scholar]
  18. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  19. Sakane T., Yokota A. 1994; Chemotaxonomic investigation of heterotrophic, aerobic and microaerophilic spirilla, the genera Aquaspirillum, Magnetospirillum and Oceanospirillum . Syst Appl Microbiol 17:128–134 [View Article]
    [Google Scholar]
  20. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  21. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  22. Terasaki Y. 1973; Studies on the genus Spirillum Ehrenberg. II. comments on type and reference strains of spirillum and description of new species and subspecies. Bull Suzugamine Women’s Coll Nat Sci 17:1–71
    [Google Scholar]
  23. Terasaki Y. 1979; Transfer of five species and two subspecies of S pirillum to other genera (Aquaspirillum and Oceanospirillum), with emended descriptions of the species and subspecies. Int J Syst Bacteriol 29:130–144 [View Article]
    [Google Scholar]
  24. Tindall B. J., Sikorski J., Smibert R. M., Krieg N. R. 2007; Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology, 3rd edn. pp 330–393 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  25. Wang G., Jia Q., Li T., Dai S., Wu H., He H., Fan J., Xiang W., Li X. 2014; Bacterioplanes sanyensis gen. nov., sp. nov., a PHB-accumulating bacterium isolated from a pool of Spirulina platensis cultivation. Arch Microbiol 196:739–744 [View Article][PubMed]
    [Google Scholar]
  26. Williams M. A., Rittenberg S. C. 1957; A taxonomic study of the genus Spirillum Ehrenberg. Int Bull Bacteriol Nomencl T axon 7:49–112
    [Google Scholar]
  27. Xie C.-H., Yokota A. 2003; Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49:345–349 [View Article][PubMed]
    [Google Scholar]
  28. Yakimov M. M., Giuliano L., Denaro R., Crisafi E., Chernikova T. N., Abraham W. R., Luensdorf H., Timmis K. N., Golyshin P. N. 2004; Thalassolituus oleivorans gen. nov., sp. nov., a novel marine bacterium that obligately utilizes hydrocarbons. Int J Syst Evol Microbiol 54:141–148 [View Article][PubMed]
    [Google Scholar]
  29. Zhang Z., Yu T., Xu T., Zhang X.-H. 2014; Aquimarina pacifica sp. nov., isolated from seawater. Int J Syst Evol Microbiol 64:1991–1997 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001461
Loading
/content/journal/ijsem/10.1099/ijsem.0.001461
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error