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Volume 66, Issue 8

sp. nov., a facultatively anaerobic, endospore-forming bacterium isolated from mineral deposits in a geothermal station Free

Abstract

A novel endospore-forming bacterium designated strain GSsed3 was isolated from deposits clogging aboveground filters from the geothermal power platform of Groß Schönebeck in northern Germany. The novel isolate was Gram-staining-positive, facultatively anaerobic, catalase-positive and oxidase-positive. Optimum growth occurred at 60 °C, 0.5 % (w/v) NaCl and pH 7–8. Analysis of the 16S rRNA gene sequence similarity indicated that strain GSsed3 belonged to the genus , and showed 99.8 % sequence similarity to R270, 98.2 % similarity to GS5-97, 97.9 % similarity to TH13, 97.7 % similarity to DSM 15730 and 97.6 % similarity to MR3C. DNA–DNA hybridization (DDH) indicated only 16 % relatedness to DSM 17127. Furthermore, DDH estimation based on genomes analysis indicated only 19.9 % overall nucleotide similarity to DSM 15939. The major respiratory menaquinone was MK-8. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unknown phosphoglycolipid and one unknown phospholipid. The predominant cellular fatty acids were iso-C, iso-C, C, iso-C and anteiso-C. The peptidoglycan type was A1γ -Dpm-direct. The genomic DNA G+C content of the strain was 46.9 mol%. The phenotypic, genotypic and chemotaxonomic characterization indicated that strain GSsed3 differs from related species of the genus. Therefore, strain GSsed3 is considered to be a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain of is GSsed3 (=CCOS808 =ATCC BAA2555).

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  • Accepted:
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Keyword(s): Anoxybacillus , endospore-former , Geothermal plant and new species
© 2016 IUMS
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2016-08-01
2024-04-19
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Anoxybacillusgeothermalis sp. nov., a facultatively anaerobic, endospore-forming bacterium isolated from mineral deposits in a geothermal station
Int J Syst Evol Microbiol 66, 2944 (2016); https://doi.org/10.1099/ijsem.0.001125
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